FLIM and FCS measurements performed with a Master Oscillator Fiber Amplifier (MOFA) laser at 530 nm

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Volumn 6860, Issue , 2008, Pages

  Koberling, Felix ^a   Langkopf, Martin ^a   Klemme, Dietmar ^a   Bülter, Andreas ^a   Buschmann, Volker ^a   Lauritsen, Kristian ^a   Erdmann, Rainer ^a  

^a PICOQUANT GMBH   (Germany)

Author keywords

Fiber amplifier; FLIM; Fluorescence lifetime imaging microscopy; MOFA; Picosecond diode lasers; Single molecule spectroscopy; SMD

Indexed keywords

COMPUTER NETWORKS; EMISSION SPECTROSCOPY; ENERGY STORAGE; ENERGY TRANSFER; FIBER AMPLIFIERS; FIBER LASERS; FIBER OPTICS; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; IMAGING TECHNIQUES; LASER EXCITATION; LASERS; LIGHT EMISSION; LUMINESCENCE; MEASUREMENTS; MEDICAL IMAGING; MICROSCOPES; MODERNIZATION; MOLECULAR SPECTROSCOPY; OPTICAL INSTRUMENTS; PULSED LASER DEPOSITION; WASTEWATER;

(P ,P ,T) MEASUREMENTS; BIOMEDICAL SCIENCES; CONFOCAL MICROSCOPES; CW LASERS; DIODE LASERS; EXCITATION WAVELENGTHS; FLUORESCENCE CORRELATION SPECTROSCOPY (FCS); FLUORESCENCE LIFE TIME IMAGING (FLIM); FLUORESCENCE RESONANCE ENERGY TRANSFER (FRETS); LASER DIODE (LD); MASTER OSCILLATORS; MEASUREMENT MODES; MULTIPHOTON MICROSCOPY (MPM); PICO SECONDS; PULSE ENERGIES; PULSED LASERS; REPETITION RATES; SPECTRAL REGIONS; STEADY-STATE MEASUREMENTS; TIME RESOLVED ANALYSIS (TRA); TIME RESOLVED MEASUREMENTS; TIME-CORRELATED SINGLE-PHOTON COUNTING (TCSPC);

PULSED LASER APPLICATIONS;

EID: 42149193134     PISSN: 16057422     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.761229     Document Type: Conference Paper

References (9)

1. Schuler, B., "Single-molecule fluorescence spectroscopy of protein folding", ChemPhysChem 06, 1206-1220 (2005)
2. Weiss, S.,"Fluorescence spectroscopy of single biomolecules", Science 283 (5408), 1676-83 (1999)
3. Rubart, M., "Two-photon microscopy of cells and tissue", Circ. Res. 95 (12), 1154-1166 (2004)
4. Lakowicz, J., "Principles of Fluorescence Spectroscopy", Springer Science+Business Media, New York, 107-117 (2006)
5. Dertinger, T., Pacheco, V., von der Hocht, I., Hartmann, R., Gregor, I., Enderlein, J., "Two-focus fluorescence correlation spectroscopy: A new tool for accurate and absolute diffusion measurements", ChemPhysChem 08, 0433-0443 (2007)
6. Rüttinger, S., Macdonald, R., Roos, M., Koberling, F., "Accurate single pair FRET through combination of Pulsed Interleaved Excitation, Time Correlated Single Photon Counting and Fluorescence Correlation Spectroscopy", J. Biomed. Opt. 11, 024012 (2006)
7. Lauritsen, K., Riecke, S., Langkopf, M., Klemme, D., Kaleva, C.M., Pallassis, C., McNeil, S., Erdmann, R.,"Fiber amplified and frequency doubled diode lasers as a highly flexible pulse source at 532nm", Proc. SPIE, 6860-57 (2008)
8. Koberling, F., Krämer, B., Kapusta, P., Patting, M., Wahl, M., Erdmann, R., "Time-resolved confocal fluorescence microscopy: novel technical features and applications for FLIM, FRET and FCS using a sophisticated data acquisition concept in TCSPC", Proc. SPIE 6583, 65830Y (2007)
9. Kapusta, P., Wahl, M., Benda, A., Hof, M., Enderlein, J., "Fluorescence lifetime correlation spectroscopy", J. Fluorescence 17, 043-048 (2007)