Whole mount enzyme histochemistry as a rapid screen at necropsy for expression of β-galactosidase (lacZ)-bearing transgenes: Considerations for separating specific lacZ activity from nonspecific (endogenous) galactosidase activity

Toxicologic Pathology

Volumn 36, Issue 2, 2008, Pages 265-276

  Bolon, Brad ^a  

^a GEMpath Inc   (United States)

Author keywords

galactosidase; Artifact; Background reaction; Enzyme histochemistry; lacZ; Transgenic mouse; Whole mount staining

Indexed keywords

BETA GALACTOSIDASE; PARAFORMALDEHYDE;

ACCESSORY SEX ORGAN; ADENOHYPOPHYSIS; ADRENAL CORTEX; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; AUTOPSY; BLADDER; BONE MARROW; BRAIN NERVE CELL; CONTROLLED STUDY; DUODENUM; ENZYME ACTIVITY; ENZYME HISTOCHEMISTRY; ENZYME LOCALIZATION; FEMALE; FROZEN SECTION; GASTROINTESTINAL TRACT; GENE EXPRESSION; GENE LOCATION; GONAD; KIDNEY; LYMPH NODE; MALE; MOUSE; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; SALIVARY GLAND; STAINING; THYMUS; THYROID GLAND; TRANSGENE; TRIGEMINUS GANGLION; UTERUS; WHOLE MOUNT ENZYME HISTOCHEMISTRY; WILD TYPE;

ANIMALS; AUTOPSY; BETA-GALACTOSIDASE; FEMALE; GENE EXPRESSION; HISTOCYTOCHEMISTRY; LAC OPERON; LUNG; MALE; MICE; MICE, INBRED C57BL; MICE, TRANSGENIC; RATS; RATS, SPRAGUE-DAWLEY; STAINING AND LABELING;

BACTERIA (MICROORGANISMS); EUKARYOTA; MUS; MUS MUSCULUS; RATTUS; RODENTIA;

EID: 42149128239     PISSN: 01926233     EISSN: 15331601     Source Type: Journal    
DOI: 10.1177/0192623307312693     Document Type: Article

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