Membrane-bound peptides from V-ATPase subunit a do not interact with an indole-type inhibitor

Journal of Peptide Science

Volumn 14, Issue 4, 2008, Pages 383-388

  Hesselink, Renske W ^a,b   Fedorov, Alexander ^a   Hemminga, Marcus A ^b   Prieto, Manuel ^a  

^a INSTITUTO SUPERIOR TÉCNICO   (Portugal)

^b WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

FRET; Subunit ; Time resolved fluorescence; Transmembrane helix 7; Transmembrane peptides; V ATPase (Vph1p); Yeast

Indexed keywords

5 (5,6 DICHLORO 2 INDOLYL) 2 METHOXY N (1,2,2,6,6 PENTAMETHYLPIPERIDIN 4 YL) 2,4 PENTADIENAMIDE; ARGININE; ENZYME INHIBITOR; LYSINE; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATASE; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATASE SUBUNIT A; SACCHAROMYCES CEREVISIAE PROTEIN; SB 242784; UNCLASSIFIED DRUG;

ARTICLE; DRUG DESIGN; DRUG MECHANISM; DRUG POTENCY; DRUG PROTEIN BINDING; DRUG STRUCTURE; DRUG TARGETING; NONHUMAN; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTON TRANSPORT; SACCHAROMYCES CEREVISIAE; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS;

ACRYLAMIDE; AMINO ACID SEQUENCE; AMINO ACIDS; ARGININE; FLUORESCENCE RESONANCE ENERGY TRANSFER; INDOLES; LIPID BILAYERS; LIPIDS; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; PEPTIDES; PHOSPHATIDYLCHOLINES; PHOSPHATIDYLGLYCEROLS; PIPERIDINES; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; SACCHAROMYCES CEREVISIAE; SPECTROMETRY, FLUORESCENCE; SPECTROPHOTOMETRY; TRYPTOPHAN; VACUOLAR PROTON-TRANSLOCATING ATPASES;

SACCHAROMYCES CEREVISIAE;

EID: 42149092185     PISSN: 10752617     EISSN: 10991387     Source Type: Journal    
DOI: 10.1002/psc.980     Document Type: Article

References (32)

1. Sambade M, Kane PM. The yeast vacuolar proton-translocating ATPase contains a subunit homologous to the Manduca sexta and bovine e subunits that is essential for function. J. Biol. Chem. 2004; 279: 17361-17365.
2. +-ATPase. Farmaco 2001; 56: 113-116.
3. +-ATPases are functionally expressed in plasma membranes of human tumor cells. Am. J. Physiol. 1993: 265: C1015-C1029.
4. Dröse S, Boddien C, Gassel M, Ingenhorst G, Zeeck A, Altendorf K. Semisynthetic: derivatives of concanamycin A and C, as inhibitors of V- and P-type ATPases: structure-activity investigations and developments of photoaffinity probes. Biochemistry 2001; 40: 12816-12825.
5. +-ATPase of osteoclasts with bone antiresorptive activity. J. Med. Chem. 1998; 41: 1568-1573.
6. Nadler G, Morvan M, Delimoge I, Belfiore P, Zocchetti A, James I, Zembryki D, Lee-Rycakzewski E, Parini C, Consolandi E, Gagliardi S, Farina C. (2Z,4E)-5-(5,6-Dichloro-2-indolyl)-2-methoxy-N- (1,2,2,6,6-pentamethylpiperidin-4-YL)-2,4-pentadienamide, a novel, potent and selective inhibitor of the osteoclast V-ATPase. Bioorg. Med. Chem. Lett. 1998; 8: 3621-3626.
7. +-ATPase prevents bone loss in both thyroparathyroidectomized and ovariectomized rats. J. Clin. Invest. 2000; 106: 309-318.
8. o-sector. Biochemistry 2004; 43: 12297-12305.
9. Dixon N, Pali T, Kee TP, Marsh D. Spin-labelled vacuolar-ATPase inhibitors in lipid membranes. Biochim. Biophys. Acta 2004; 1665: 177-183.
10. Fernandes F, Loura L, Koehorst RBM, Dixon N, Kee TP, Hemminga MA, Prieto M. Interaction of the indole class of V-ATPase inhibitors with lipid bilayers. Biochemistry 2006; 45: 5271-5279.
11. +-ATPase bind with high affinity to the purified proteolipid subunit of the membrane domain. Biochemistry 2005; 44: 15024-15031.
12. +-V-ATPase includes a flexible region that has a potential role in proton translocation. Biochim. Biophys. Acta 2007; 1768: 2263-2270.
13. Kawasaki-Nishi S, Nishi T, Forgac M. Arg-735 of the 100-kDa subunit a of the yeast V-ATPase is essential for proton translocation. Proc. Natl. Acad. Sci. U.S.A. 2001; 98: 12397-12402.
14. Kawasaki-Nishi S, Nishi T, Forgac M. Interacting helical surfaces of the transmembrane segments of subunits a and c' of the yeast V-ATPase defined by disulfide-mediated cross-linking. J. Biol. Chem. 2003; 278: 41908-41913.
15. Kawasaki-Nishi S, Nishi T, Forgac M. Proton translocation driven by ATP hydrolysis in V-ATPases. FEBS Lett. 2003; 545: 76-85.
16. +)-ATPases-Nature's most versatile proton pumps. Nat. Rev. Mol. Cell Biol. 2002; 3: 94-103.
17. Hesselink RW, Koehorst RBM, Nazarov PV, Hemminga MA. Membrane-bound peptides mimicking transmembrane Vph1p helix 7 of yeast V-ATPase: A spectroscopic and polarity mismatch study. Biochim. Biophys. Acta 2005; 1716: 137-145.
18. Fernandes F, Loura LMS, Fedorov A, Dixon N, Kee TP, Prieto M, Hemminga MA. Binding assays of inhibitors towards selected V-ATPase domains. Biochim. Biophys. Acta 2006; 1758: 1777-1786.
19. de Planque MRR, Killian JA. Protein-lipid interactions studied with designed transmembrane peptides: Role of hydrophobic matching and interfacial anchoring. Mol. Membr. Biol. 2003; 20: 271-284.
20. Castanho MARB, Santos NC, Loura LMS. Separating the turbidity spectra of vesicles from the absorption spectra of membrane probes and other chromophores. Eur. Biophys. J. 1997; 26: 253-259.
21. Loura LMS, Fedorov A, Prieto M. Membrane probe distribution heterogeneity: a resonance energy transfer study. J. Phys. Chem. B 2000; 104: 6920-6931.
22. Marquardt DW. An algorithm for least-squares estimation of nonlinear parameters. J. Soc. Ind. Appl. Math. 1963; 11: 431-441.
23. Lakowicz JR. Principles of fluorescence Spectroscopy, 2nd edn. Kluwer Academic/Plenum Publishers: New York, 1999.
24. Wiener MC, White SH. Structure of a fluid dioleoylphosphatidylcholine bilayer determined by joint refinement of X-ray and neutron-diffraction data. 3. Complete structure. Biophys. J. 1992; 61: 437-447.
25. Fernandes F, Loura LMS, Koehorst RBM, Spruijt RB, Hemminga MA, Fedorov A. Prieto M. Quantification of protein-lipid selectivity using FRET: Application to the M13 major coat protein. Biophys. J. 2004; 87: 344-352.
26. Strandberg E, Killian JA. Snorkeling of lysine side chains in transmembrane helices: How easy can it get? FEBS Lett. 2003; 544: 69-73.
27. Umberger JQ, Lamer VK. The kinetics of diffusion-controlled molecular and ionic reactions in solution as determined by measurements of the quenching of fluorescence. J. Am. Chem. Soc. 1945; 67: 1099-1109.
28. de Almeida RFM, Loura LMS, Prieto M, Watts A, Fedorov A, Barrantes FJ. Cholesterol modulates the organization of the γM4 transmembrane domain of the muscle nicotinic acetylcholine receptor. Biophys. J. 2004; 86: 2261-2272.
29. Fernandes F, Loura LMS, Prieto M, Koehorst R, Spruijt RB, Hemminga MA. Dependence of M13 major coat protein oligomerization and lateral segregation on bilayer composition. Biophys. J. 2003; 85: 2430-2441.
30. Bowman BJ, Bowman EJ. Mutations in subunit c of the vacuolar ATPase confer resistance to bafilomycin and identify a conserved antibiotic binding site. J. Biol. Chem. 2002; 277: 3965-3972.
31. Bowman EJ. Graham LA, Stevens TH, Bowman BJ. The bafilomycin/ concanamycin binding site in subunit c of the V-ATPase from Neurospora crassa and Saccharomyces cerevisiae. J. Biol. Chem. 2004; 279: 33131-33138.
32. o subunit c. J. Biol. Chem. 2002; 277: 40544-40548.