Determining post-thaw CD34+ cell dose of cryopreserved haematopoietic progenitor cells demonstrates high recovery and confirms their integrity

Vox Sanguinis

Volumn 94, Issue 4, 2008, Pages 351-357

  Reich Slotky, R ^a   Colovai, A I ^a   Semidei Pomales, M ^a   Patel, N ^a   Cairo, M ^a   Jhang, J ^a   Schwartz, J ^a  

^a NewYork Presbyterian Hospital   (United States)

Author keywords

CD34+; Cryopreservation; Hematopoietic progenitor cell; Viability

Indexed keywords

DIMETHYL SULFOXIDE;

ADULT; ARTICLE; CD34 SELECTION; CELL SURVIVAL; CELL VIABILITY; CLINICAL ARTICLE; CONTROLLED STUDY; CRYOPRESERVATION; FEMALE; GRANULOCYTE; HEMATOPOIETIC CELL; HUMAN; HUMAN CELL; MALE; PRIORITY JOURNAL; STEM CELL;

ADULT; ANTIGENS, CD34; CELL COUNT; CELL SURVIVAL; CRYOPRESERVATION; FEMALE; GRAFT SURVIVAL; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HEMATOPOIETIC STEM CELLS; HUMANS; MALE; MIDDLE AGED; TRANSPLANTATION, AUTOLOGOUS; TRANSPLANTATION, HOMOLOGOUS;

EID: 42149091099     PISSN: 00429007     EISSN: 14230410     Source Type: Journal    
DOI: 10.1111/j.1423-0410.2007.001028.x     Document Type: Article

References (30)

1. Mehta J, Powles R, Treleaven J, Kulkarni S, Horton C, Singhal S : Number of nucleated cells infused during allogeneic and autologous bone marrow transplantation: an important modifiable factor influencing outcome. Blood 1997 90 : 3808 3810
2. Moore MA, Williams N, Metcalf D : In vitro colony formation by normal and leukemic human hematopoietic cells: characterization of the colony-forming cells. J Natl Cancer Inst 1973 50 : 603 623
3. Emminger W, Emminger-Schmidmeier W, Hocker P, Hinterberger W, Gadner H : Myeloid progenitor cells (CFU-GM) predict engraftment kinetics in autologous transplantation in children. Bone Marrow Transplant 1989 4 : 415 420
4. Rowley SD, Zuehlsdorf M, Braine HG, Colvin OM, Davis J, Jones RJ, Saral R, Sensenbrenner LL, Yeager A, Santos GW : CFU-GM content of bone marrow graft correlates with time to hematologic reconstitution following autologous bone marrow transplantation with 4-hydroperoxycyclophosphamide-purged bone marrow. Blood 1987 70 : 271 275
5. Sutherland DR, Keating A : The CD34 antigen: structure, biology, and potential clinical applications. J Hematother 1992 1 : 115 129
6. Krause DS, Fackler MJ, Civin CI, May WS : CD34: structure, biology, and clinical utility. Blood 1996 87 : 1 13
7. Jansen J, Hanks S, Thompson JM, Dugan MJ, Akard LP : Transplantation of hematopoietic stem cells from the peripheral blood. J Cell Mol Med 2005 9 : 37 50
8. Ketterer N, Salles G, Raba M, Espinouse D, Sonet A, Tremisi P, Dumontet C, Moullet I, Eljaafari-Corbin A, Neidhardt-Berard EM, Bouafia F, Coiffier B : High CD34(+) cell counts decrease hematologic toxicity of autologous peripheral blood progenitor cell transplantation. Blood 1998 91 : 3148 3155
9. Allan DS, Keeney M, Howson-Jan K, Popma J, Weir K, Bhatia M, Sutherland DR, Chin-Yee IH : Number of viable CD34(+) cells reinfused predicts engraftment in autologous hematopoietic stem cell transplantation. Bone Marrow Transplant 2002 29 : 967 972
10. Haas R, Witt B, Mohle R, Goldschmidt H, Hohaus S, Fruehauf S, Wannenmacher M, Hunstein W : Sustained long-term hematopoiesis after myeloablative therapy with peripheral blood progenitor cell support. Blood 1995 85 : 3754 3761
11. Bittencourt H, Rocha V, Chevret S, Socie G, Esperou H, Devergie A, Dal Cortivo L, Marolleau JP, Garnier F, Ribaud P, Gluckman E : Association of CD34 cell dose with hematopoietic recovery, infections, and other outcomes after HLA-identical sibling bone marrow transplantation. Blood 2002 99 : 2726 2733
12. Abrahamsen JF, Wentzel-Larsen T, Bruserud O : Autologous transplantation: the viable transplanted CD34+ cell dose measured post-thaw does not predict engraftment kinetics better than the total CD34+ cell dose measured pre-freeze in patients that receive more than 2 × 10 (6) CD34+ cells/kg. Cytotherapy 2004 6 : 356 362
13. Reeb B : Dye exclusion test for bone marrow viability in Areman EM DH, Sacher RA (eds) : Bone Marrow and Stem Cell Processing: A Manual of Current Techniqes. New York, FA Davis, 1992 : 403 404
14. Keeney M, Chin-Yee I, Weir K, Popma J, Nayar R, Sutherland DR : Single platform flow cytometric absolute CD34+ cell counts based on the ISHAGE guidelines. International Society of Hematotherapy and Graft Engineering. Cytometry 1998 34 : 61 70
15. Schmid I, Hausner MA, Cole SW, Uittenbogaart CH, Giorgi JV, Jamieson BD : Simultaneous flow cytometric measurement of viability and lymphocyte subset proliferation. J Immunol Methods 2001 247 : 175 186
16. Schuurhuis GJ, Muijen MM, Oberink JW, de Boer F, Ossenkoppele GJ, Broxterman HJ : Large populations of non-clonogenic early apoptotic CD34-positive cells are present in frozen-thawed peripheral blood stem cell transplants. Bone Marrow Transplant 2001 27 : 487 498
17. Humpe A, Beck C, Schoch R, Kneba M, Horst HA : Establishment and optimization of a flow cytometric method for evaluation of viability of CD34+ cells after cryopreservation and comparison with trypan blue exclusion staining. Transfusion 2005 45 : 1208 1213
18. Martin-Henao GA, Picon M, Amill B, Querol S, Gonzalez JR, Martinez C, Martino R, Ferra C, Brunet S, Granena A, Sierra J, Garcia J : Isolation of CD34+ progenitor cells from peripheral blood by use of an automated immunomagnetic selection system: factors affecting the results. Transfusion 2000 40 : 35 43
19. Gutensohn K, Carrero I, Krueger W, Kroeger N, Schafer P, Luedemann K, Kuehnl P : Semi-automated flow cytometric analysis of CD34-expressing hematopoietic cells in peripheral blood progenitor cell apheresis products. Transfusion 1999 39 : 1220 1226
20. Schmid I, Krall WJ, Uittenbogaart CH, Braun J, Giorgi JV : Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry. Cytometry 1992 13 : 204 208
21. Gao DY, Chang Q, Liu C, Farris K, Harvey K, McGann LE, English D, Jansen J, Critser JK : Fundamental cryobiology of human hematopoietic progenitor cells. I: Osmotic characteristics and volume distribution. Cryobiology 1998 36 : 40 48
22. Fietz T, Reufi B, Mucke C, Thiel E, Knauf WU : Flow cytometric CD34+ determination in stem cell transplantation: before or after cryopreservation of grafts? J Hematother Stem Cell Res 2002 11 : 429 435
23. Gratama JW, Orfao A, Barnett D, Brando B, Huber A, Janossy G, Johnsen HE, Keeney M, Marti GE, Preijers F, Rothe G, Serke S, Sutherland DR, Van der Schoot CE, Schmitz G, Papa S : Flow cytometric enumeration of CD34+ hematopoietic stem and progenitor cells. European Working Group on Clinical Cell Analysis. Cytometry 1998 34 : 128 142
24. Feugier P, Bensoussan D, Girard F, Alla F, Schuhmacher A, Latger-Cannard V, Hulin C, Witz F, Witz B, Carret AS, Bordigoni P, Lederlin P, Janot C : Hematologic recovery after autologous PBPC transplantation: importance of the number of postthaw CD34+ cells. Transfusion 2003 43 : 878 884
25. Tarasov AI, Petrenko AY, Jones DR : The osmotic characteristics of human fetal liver-derived hematopoietic stem cell candidates. Cryobiology 2004 48 : 333 340
26. Xiao M, Dooley DC : Assessment of cell viability and apoptosis in human umbilical cord blood following storage. J Hematother Stem Cell Res 2003 12 : 115 122
27. Mascotti K, McCullough J, Burger SR : HPC viability measurement: trypan blue versus acridine orange and propidium iodide. Transfusion 2000 40 : 693 696
28. Sartor M, Antonenas V, Garvin F, Webb M, Bradstock KF : Recovery of viable CD34+ cells from cryopreserved hemopoietic progenitor cell products. Bone Marrow Transplant 2005 36 : 199 204
29. Humpe A, Riggert J, Vehmeyer K, Troff C, Hiddemann W, Kohler M, Wormann B : Comparison of CD34+ cell numbers and colony growth before and after cryopreservation of peripheral blood progenitor and stem cell harvests: influence of prior chemotherapy. Transfusion 1997 37 : 1050 1057
30. Ojeda-Uribe M, Sovalat H, Bourderont D, Brunot A, Marr A, Lewandowski H, Chaboute V, Peter P, Henon P : Peripheral blood BM CD34+ CD38- cells show better resistance to cryopreservation than CD34+ CD38+ cells in autologous stem cell transplantation. Cytotherapy 2004 6 : 571 583