Enzymatic permeabilization of the thecate dinoflagellate Alexandrium minutum (Dinophyceae) yields detection of intracellularly associated bacteria via catalyzed reporter deposition-fluorescence in situ hybridization

Applied and Environmental Microbiology

Volumn 74, Issue 7, 2008, Pages 2244-2247

  Palacios, Lucia ^a   Marín, Irma ^a  

^a UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; CONFOCAL MICROSCOPY; DEPOSITION; ENZYME ACTIVITY; FLUORESCENCE;

ALEXANDRIUM MINUTUM; BACTEROIDETES; FLUORESCENT BACTERIAL PROBES; HYBRIDIZATION; LASER SCANNING MICROSCOPY;

BACTERIA;

PROPIDIUM IODIDE;

BACTERIUM; DINOFLAGELLATE; DYE; ENZYME ACTIVITY; FLUORESCENCE; HYBRIDIZATION; IN SITU MEASUREMENT; IODIDE; MICROSCOPY; PERMEABILITY;

ALEXANDRIUM MINUTUM; ARTICLE; BACTERIUM DETECTION; CATALYSIS; CELL PERMEABILIZATION; CONFOCAL LASER MICROSCOPY; DINOFLAGELLATE; FLUORESCENCE IN SITU HYBRIDIZATION; NONHUMAN;

ANIMALS; BACTERIA; DINOFLAGELLIDA; IN SITU HYBRIDIZATION, FLUORESCENCE; MICROSCOPY, CONFOCAL;

ALEXANDRIUM MINUTUM; BACTERIA (MICROORGANISMS); BACTEROIDETES; DINOPHYCEAE;

EID: 42049092740     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.01144-07     Document Type: Article

References (29)

1. Alverca, E., I. C. Biegala, G. M. Kennaway, J. Lewis, and S. Franca. 2002. In situ identification and localization of bacteria associated with Gyrodinium instriatum (Gymnodiales, Dinophyceae) by electron and confocal microscopy. Eur. J. Phycol. 37:523-530.
2. Amann, R. I., L. Krumholz, and D. A. Stahl. 1990. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J. Bacteriol. 172:762-770.
3. Amann, R. I., B. Zarda, D. A. Stahl, and K.-H. Schleifer. 1992. Identification of individual prokaryotic cells by using enzyme-labeled, rRNA-targeted oligonucleotide probes. Appl. Environ. Microbiol. 58:3007-3011.
4. Amaro, A. M., M. S. Fuentes, S. R. Ogalde, J. A. Venegas, and B. J. Suárez-Isla. 2005. Identification and characterization of potentially algal-lytic marine bacteria strongly associated with the toxic dinoflagellate Alexandrium catenella. J. Eukaryot. Microbiol. 52:191-200.
5. Baker, T. R., G. J. Doucette, C. L. Powell, G. L. Boyer, and F. G. Plumley. 2003. GTX4 imposters: characterization of fluorescence compounds synthesized by Pseudoalteromonas stutzeri SF/P and Pseudomonas/ Alteromonas PTB-1, symbionts of saxitoxin-producing Alexandrium spp. Toxicon 41:339-347.
6. Bell, W. H., and R. Mitchell. 1972. Chemotactic and growth responses of marine bacteria to algal extracellular products. Biol. Bull. 143:265-277.
7. Biegala, I., G. Kennaway, E. Alverca, J.-F. Lennon, D. Vaulot, and N. Simon. 2002. Identification of bacteria associated with dinoflagellates (Dinophyceae) Alexandrium spp. using tyramide signal amplification- fluorescence in situ hybridization and confocal microscopy. J. Phycol. 38:404-411.
8. Doucette, G. J., and C. L. Powell. 1998. Algal-bacterial interactions: can they determine the PSP-related toxicity of dinoflagellates?, p. 406-409. In B. Reguera, J. Blanco, M. L. Fernández, and T. Wyatt (ed.), Harmful algae. Xunta de Galicia and Intergovernmental Oceanographic Commission of UNESCO, Santiago de Compostela, Spain.
9. Doucette, G. J., E. R. McGovern, and J. A. Babinchak. 1999. Alguicidal bacteria active against Gymnodinium breve (Dinophyceae). I. Bacterial isolation and characterisation of killing activity. J. Phycol. 35:1447-1454.
10. Furuki, M., and M. Kobayashi. 1991. Interactions between Chattonella and bacteria and prevention of this red tide - EMECS '90. Mar. Pollut. Bull. 23:189-193.
11. Gallacher, S., and E. A. Smith. 1999. Bacteria and paralytic shellfish toxins. Protist 150:245-255.
12. Gold, K., and U. Polingher. 1971. Occurrence of endosymbiotic bacteria in marine dinoflagellates. J. Phycol. 7:264-265.
13. Guillard, R. R. L., and P. E. Hargraves. 1993. Stichochrysis immobilis is a diatom, not a chrysophyte. Phycologia 32:234-236.
14. Hold, G. L., E. A. Smith, T. H. Birkbeck, and S. Gallacher. 2001. Comparison of paralytic shellfish toxin (PST) production by the dinoflagellates Alexandrium lusitanicum NEPCC 253 and Alexandrium tamarense NEPCC 407 in the presence and absence of bacteria. FEMS Microbiol. Ecol. 36:223-234.
15. Kim, M. C., I. Yoshinaga, I. Imai, K. Nagasaki, S. Itakura, and Y. Ishida. 1998. A close relationship between alguicidal bacteria and termination of Heterosigma akashiwo (Raphidophyceae) bloom in Hiroshima Bay, Japan. Mar. Ecol. Prog. Ser. 170:25-32.
16. Lewis, J., G. Kennaway, S. Franca, and E. Alverca. 2001. Bacteria-dinoflagellate interactions: investigative microscopy of Alexandrium spp. (Gonyaulacales. Dinophyceae). Phycologia 40:280-285.
17. Lovejoy, C., J. P. Bowman, and G. M. Hallegraeff. 1998. Alguicidal effects of a novel marine Pseudoalteromonas isolate (class Proteobacteria, Gamma subdivision) on harmful algal bloom species of the genera Chattonella, Gymnodinium, and Heterosigma. Appl. Environ. Microbiol. 64:2806-2813.
18. Lucas, I. A N. 1982. Observations on Noctiluca scintillians Macartney (Ehrenb.) (Dinophyceae) with notes on an intracellular bacterium. J. Plankton Res. 4:401-409.
19. Manz, W., R. Amann, W. Ludwig, M. Vancanneyt, and K.-H. Schleider. 1996. Application of a suite of 16S rRNA-specific oligonucleotide probes designed to investigate bacteria of the phylum Cytophaga-Flavobacterium- Bacteroides in the natural environment. Microbiology 142:1097-1106.
20. Mayali, X., and F. Azam. 2004. Alguicidal bacteria in the sea and their impact on algal bloom. J. Eukaryot. Microbiol. 51:139-144.
21. Palacios, L., D. Arahal, B. Reguera, and I. Marin. 2006. Hoeflea alexandrii sp. nov., isolated from the toxic dinoflagellate Alexandrium minutum AL1V. J. Syst. Evol. Microbiol. 56:1991-1995.
22. Pernthaler, A., J. Pernthaler, and R. Amann. 2002. Fluorescence in situ hybridization and catalyzed reporter deposition for the identification of marine bacteria. Appl. Environ. Microbiol. 68:3094-3101.
23. Rausch de Traunbenberg, C., M.-L. Geraud, M. O. Soyer-Gobillard, and D. Emdadi. 1995. The toxic dinoflagellate Prorocentrum lima and its associated bacteria. Eur. J. Protistol. 31:318-326.
24. Riemann, L., G. F. Steward, and F. Azam. 2000. Dynamics of bacterial community composition and activity during a mesocosm diatom bloom. Appl. Environ. Microbiol. 66:318-326.
25. Silva, E. S. 1962. Some observations on marine dinoflagellate cultures. I. Prorocentrum micans Ehr. and Gymnodinium spinifera (Clap.and Lach.) dies., Gonyaulax tamarensis Leb., and Peridinium trochoideum (Stein) Lemm. Notas Estudos Inst. Biol. Mar. 26:1-21.
26. Tujula, N. A., C. Holmstöm, M. Mußmann, R. Amann, S. Kjelleberg, and G. R. Crocetti. 2006. A CARD-FISH protocol for the identification and enumeration of epiphytic bacteria on marine algae. J. Microbiol. Methods 65:604-607.
27. Urdea, M. S., B. D. Warner, J. A. Running, M. Stempien, J. Clyne, and T. Horn. 1998. A comparison of nonradioisotopic hybridization assay methods using fluorescent, chemiluminiscent and enzyme labelled synthetic oligodeoxyribonucleotide probes. Nucleic Acids Res. 16:4937-4956.
28. Uribe, P., and R. T. Espejo. 2003. Effect of associated bacteria on the growth and toxicity of Alexandrium catenella. Appl. Environ. Microbiol. 69:659-662.
29. Wang, C. H., Y. Y. Wang, Y. Y. Sun, and X. T. Xie. 2006. Effect of antibiotic treatment on toxin production by Alexandrium tamarense. Biomed. Environ. Sci. 16:340-347.