A sensitive three-step protocol for fluorescence-based Western blot detection

Journal of Immunological Methods

Volumn 334, Issue 1-2, 2008, Pages 51-58

  Delaive, Edouard ^a   Arnould, Thierry ^a   Raes, Martine ^a   Renard, Patricia ^a  

^a UNIVERSITY OF NAMUR   (Belgium)

Author keywords

Fluorescent conjugate; Three step procedure; Western blot detection

Indexed keywords

ANTIBODY CONJUGATE; BIOTIN ANTIBODY; CYTOKERATIN 8; MITOGEN ACTIVATED PROTEIN KINASE P38; PROTEIN ANTIBODY; STREPTAVIDIN; UNCLASSIFIED DRUG;

ANTIGEN DETECTION; ARTICLE; BIOTINYLATION; CONJUGATION; FLUORESCENCE; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN ANALYSIS; SENSITIVITY ANALYSIS; WESTERN BLOTTING;

ANTIBODIES; BIOTIN; BLOTTING, WESTERN; CELL LINE; FLUORESCENCE; FLUORESCENT DYES; FLUOROIMMUNOASSAY; HUMANS; KERATIN-8; KERATINOCYTES; NF-KAPPA B; P38 MITOGEN-ACTIVATED PROTEIN KINASES; SENSITIVITY AND SPECIFICITY; STREPTAVIDIN;

EID: 41949097627     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2008.02.007     Document Type: Article

References (11)

1. Amersham Bioscience. Application Note: Multiplex Detection Using the ECL Plex Fluorescent Western Blotting System (2006) Code : # 28-4015-40
2. Cao Y., Christian S., and Suresh M.R. Development of a bispecific monoclonal antibody as a universal immunoprobe for detecting biotinylated macromolecules. J. Immunol. Methods 220 (1998) 85
3. Franzen P., Ichijo H., and Miyazono K. Different signals mediate transforming growth factor-beta 1-induced growth inhibition and extracellular matrix production in prostatic carcinoma cells. Exp. Cell Res. 207 (1993) 1
4. Gingrich J.C., Davis D.R., and Nguyen Q. Multiplex detection and quantitation of proteins on western blots using fluorescent probes. Biotechniques 29 (2000) 636
5. Green N.M. Avidin 1. The use of [14C]biotin for kinetic studies and for assay. Biochem. J. 89 (1963) 585
6. Lewis B., Rathman S., and McMahon R.J. Detection and quantification of biotinylated proteins using the Storm 840 optical scanner. J. Nutr. Biochem. 14 (2003) 196
7. Lilley K.S., and Friedman D.B. All about DIGE: quantification technology for differential-display 2D-gel proteomics. Expert Rev. Proteomics 1 (2004) 401
8. Rheinwald J.G., Hahn W.C., Ramsey M.R., Wu J.Y., Guo Z., Tsao H., De Luca M., Catricala C., and O'Toole K.M. A two-stage, p16(INK4A)- and p53-dependent keratinocyte senescence mechanism that limits replicative potential independent of telomere status. Mol. Cell. Biol. 22 (2002) 5157
9. Towbin H., Staehelin T., and Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76 (1979) 4350
10. Vankoningsloo S., De Pauw A., Houbion A., Tejerina S., Demazy C., de Longueville F., Bertholet V., Renard P., Remacle J., Holvoet P., Raes M., and Arnould T. CREB activation induced by mitochondrial dysfunction triggers triglyceride accumulation in 3T3-L1 preadipocytes. J. Cell Sci. 119 (2006) 1266
11. Vincent P., and Samuel D. A comparison of the binding of biotin and biotinylated macromolecular ligands to an anti-biotin monoclonal antibody and to streptavidin. J. Immunol. Methods 165 (1993) 177