Interlaboratory comparison of quantitative RT-PCR based detection for minimal residual disease in leukemias: A standardization approach in Japan

Tohoku Journal of Experimental Medicine

Volumn 214, Issue 2, 2008, Pages 97-104

  Yamada, Minami F ^a   Fujiwara, Tohru ^a   Ishikawa, Izumi ^b   Kohata, Katsura ^a   Katoh, Chiaki ^c   Miyamura, Koichi ^a   Harigae, Hideo ^a  

^a TOHOKU UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b Osaki Citizen Hospital   (Japan)

^c NATIONAL HOSPITAL ORGANIZATION NAGOYA MEDICAL CENTER   (Japan)

Author keywords

Blood separation; Hematological malignancies; Interlaboratory variables; Minimal residual disease (MRD); RQ PCR

Indexed keywords

BCR ABL PROTEIN; TRANSCRIPTION FACTOR RUNX1;

ACUTE GRANULOCYTIC LEUKEMIA; ARTICLE; BLOOD ANALYSIS; BONE MARROW EXAMINATION; CHIMERA; CHRONIC MYELOID LEUKEMIA; COMPARATIVE STUDY; CONTROLLED STUDY; DNA SYNTHESIS; HUMAN; JAPAN; LABORATORY; LABORATORY TEST; LEUKEMIA; MINIMAL RESIDUAL DISEASE; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA EXTRACTION; STANDARDIZATION; UNIVERSITY HOSPITAL;

HUMANS; JAPAN; LEUKEMIA; NEOPLASM, RESIDUAL; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 41949090546     PISSN: 00408727     EISSN: 13493329     Source Type: Journal    
DOI: 10.1620/tjem.214.97     Document Type: Article

References (12)

1. Beillard, E., Pallisgaard, N., van der Velden, V.H., Bi, W., Dee, R., van der Schoot, E., Delabesse, E., Macintyre, E., Gottardi, E., Saglio, G., Watzinger, F., Lion, T., van Dongen, J.J., Hokland, P. & Gabert, J. (2003) Evaluation of candidate control genes for diagnosis and residual disease detection in leukemic patients using 'real-time' quantitative reverse-transcriptase polymerase chain reaction (RQ-PCR) - a Europe against cancer program. Leukemia, 17, 2474-2486.
2. Bustin, S.A. & Nolan, T. (2004) Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. J. Biomol. Tech., 15, 155-166.
3. Bustin, S.A., Benes, V., Nolan, T. & Pfaffl, M.W. (2005) Quantitative real-time RT-PCR - a perspective. J. Mol. Endocrinol., 34, 597-601.
4. Gabert, J., Beillard, E., van der Velden, V.H., Bi, W., Grimwade, D., Pallisgaard, N., Barbany, G., Cazzaniga, G., Cayuela, J.M., Cave, H., Pane, F., Aerts, J.L., De Micheli, D., Thirion, X., Pradel, V., Gonzalez, M., Viehmann, S., Malec, M., Saglio. G. & van Dongen J. J. (2003) Standardization and quality control studies of 'real-time' quantitative reverse transcriptase polymerase chain reaction of fusion gene transcripts for residual disease detection in leukemia - a Europe Against Cancer program. Leukemia, 17, 2318-2357.
5. Hughes, T.P., Kaeda, J., Branfordm, S., Rudzki, Z., Hochhaus, A., Hensley, M.L., Gathmann, I., Bolton, A.E., van Hoomissen, I.C., Goldmanm J.M. & Radich, J.P.; International Randomised Study of Interferon versus STI571 (IRIS) Study Group (2003) Frequency of major molecular responses to imatinib or interferon alfa plus cytarabine in newly diagnosed chronic myeloid leukemia. N. Engl. J. Med., 349, 1423-1432.
6. Hughes, T., Deininger, M., Hochhaus, A., Branford, S., Radich, J., Kaeda, J., Baccarani, M., Cortes, J., Cross, N.C., Druker, B.J., Gabert, J., Grimwade, D., Hehlmann, R., Kamel-Reid, S., Lipton, J.H., Longtine, J., Martinelli, G., Saglio, G., Soverini, S., Stock, W. & Goldman, J.M. (2006) Monitoring CML patients responding to treatment with tyrosine kinase inhibitors: review and recommendations for harmonizing current methodology for detecting BCR-ABL transcripts and kinase domain mutations and for expressing results. Blood, 108, 28-37.
7. Müller, M.C., Merx, K., Weisser, A., Kreil, S., Lahaye, T., Hehlmann, R. & Hochhaus, A. (2002) Improvement of molecular munitoring of residual disease in leukemias by bedside RNA stabilization. Leukemia, 16, 2395-2399.
8. Müller, M.C., Erben, P., Saglio, G., Gottardi, E., Nyvold, C.G., Schenk, T., Ernst, T., Lauber, S., Kruth, J., Hehlmann, R. & Hochhaus, A. (2005) Harmonization of BCR-ABL mRNA quantification using an uniform control plasmid in 36 international laboratories. Blood, 106, 563 (Abstract).
9. Prezeau, N., Silvy, M., Gabert, J. & Picard, C. (2006) Assessment of a new RNA stabilizing reagent (Tempus Blood RNA) for minimal residual disease in onco-hematology using the EAC protocol. Leuk. Res., 30, 569-574.
10. Tamul, K.R., Schmitz, J.L., Kane, K. & Folds, J.D. (1995) Comparison of the effects of Ficoll-Hypaque separation and whole blood lysis on results of immunophenotypic analysis of blood and bone marrow samples from patients with hematologic malignancies. Clin. Diagn. Lab. Immunol., 2, 337-342.
11. van Dongen, J.J., Macintyre, E.A., Gabert, J.A., Delabesse, E., Rossi, V., Saglio, G., Gottardi, E., Rambaldi, A., Dotti, G., Griesinger, F., Parreira, A., Gameiro, P., Diaz, M.G., Malec, M., Langerak, A.W., San Miguel, J.F. & Biondi, A. (1999) Standardized RT-PCR analysis of fusion gene transcripts from chromosome aberrations in acute leukemia for detection of minimal residual disease. Report of the BIOMED-1 Concerted Action: investigation of minimal residual disease in acute leukemia. Leukemia, 13, 1901-1928.
12. Wang, Y.L., Lee, J.W., Cesarman, E., Jin, D.K. & Csernus, B. (2006) Molecular monitoring of chronic myelogenous leukemia: identification of the most suitable internal control gene for real-time quantification of BCR-ABL transcripts. J. Mol. Diagn., 8, 231-239.