A novel assay system for myeloperoxidase activity in whole saliva

Clinical Biochemistry

Volumn 41, Issue 7-8, 2008, Pages 584-590

  Sakamoto, Wataru ^a,b   Fujii, Yoshihiro ^a   Kanehira, Takashi ^c   Asano, Kozo ^c   Izumi, Hiroshi ^d  

^a FUJI WOMEN'S UNIVERSITY   (Japan)

^b Serotec Laboratory   (Japan)

^c HOKKAIDO UNIVERSITY   (Japan)

^d HEALTH SCIENCES UNIVERSITY OF HOKKAIDO   (Japan)

Author keywords

"Sandwich" test disk; DAB (3,3 diaminobenzidine); Dapsone (4,4 diaminodiphenylsulfone); Guaiacol; MPO (myeloperoxidase); Periodontal disease; Saliva; Salivary peroxidase

Indexed keywords

4 DIMETHYLAMINOAZOBENZENE; DAPSONE; DIETHYLAMINOETHYL CELLULOSE; GUAIACOL; HYDROGEN PEROXIDE; MYELOPEROXIDASE; PEROXIDASE; TROMETAMOL;

ADULT; AGED; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; ENZYMATIC ASSAY; ENZYME ACTIVITY; FEMALE; HUMAN; MALE; NEUTROPHIL; PERIODONTAL DISEASE; PRIORITY JOURNAL; ROOM TEMPERATURE; SALIVA;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; CHROMATOGRAPHY, DEAE-CELLULOSE; ENZYME ACTIVATION; FEMALE; HUMANS; MALE; MIDDLE AGED; PERIODONTAL DISEASES; PEROXIDASE; SALIVA; SALIVARY PROTEINS;

EID: 41849139991     PISSN: 00099120     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.clinbiochem.2007.12.025     Document Type: Article

References (32)

1. Ihalin R., Loimaranta V., and Tenovuo J. Origin, structure, and biological activities of peroxidases in human saliva. Arch Biochem Biophys 445 (2006) 261-268
2. Nagler R.M., Klein I., Zarzhevsky N., Drigues N., and Reznick A.Z. Characterization of the differentiated antioxidant profile of human saliva. Free Radic Biol Med 32 (2002) 268-277
3. Deby-Dupont G., Deby C., and Lamy M. Neutrophil myeloperoxidase revisited: it's role in health and disease. Intensivmed 36 (1999) 500-513
4. Klebanoff S.J. Myeloperoxidase: friend and foe. J Leukoc Biol 77 (2005) 598-625
5. Smith Q.T., Hinrichs J.E., and Melnyk R.S. Gingival crevicular fluid myeloperoxidase at periodontitis sites. J Periodontal Res 21 (1986) 45-55
6. Cao C.F., and Smith Q.T. Crevicular fluid myeloperoxidase at healthy, gingivitis and periodontitis sites. J Clin Periodontol 16 (1989) 17-20
7. Wei P.F., Ho K.Y., Ho Y.P., Wu Y.M., Yang Y.H., and Tsai C.C. The investigation of glutathione peroxidase, lactoferrin, myeloperoxidase and interleukin-1beta in gingival crevicular fluid: implications for oxidative stress in human periodontal diseases. J Periodontal Res 39 (2004) 287-293
8. Ximenez-Fyvie L.A., Haffajee A.D., and Socransky S.S. Comparison of the microbiota of supra- and subgingival plaque in health and periodontitis. J Clin Periodontol 27 (2000) 648-657
9. Lamster I.B., Kaufman E., Grbic J.T., Winston L.J., and Singer R.E. Beta-glucuronidase activity in saliva: relationship to clinical periodontal parameters. J Periodontol 74 (2003) 353-359
10. Hagewald S., Bernimoulin J.P., Kottgen E., and Kage A. Salivary IgA subclasses and bacteria-reactive IgA in patients with aggressive periodontitis. J Periodontal Res 37 (2002) 333-339
11. Yamalik N., Caglayan F., Kilinc K., Kilinc A., and Tumer C. The importance of data presentation regarding gingival crevicular fluid myeloperoxidase and elastase-like activity in periodontal disease and health status. J Periodontol 71 (2000) 460-467
12. Bodet C., Chandad F., and Grenier D. Porphyromonas gingivalis-induced inflammatory mediator profile in an ex vivo human whole blood model. Clin Exp Immunol 143 (2006) 50-57
13. Malle E., Furtmuller P.G., Sattler W., and Obinger C. Myeloperoxidase: a target for new drug development?. Br J Pharmacol 152 (2007) 838-854
14. Kaner D., Bernimoulin J.P., Kleber B.M., Heizman W.R., and Friedmann A. Gingival crevicular fluid levels of calprotectin and myeloperoxidase during therapy for generalized aggressive periodontitis. J Periodontal Res 41 (2006) 132-139
15. Miller C.S., King C.P., Langub M.C., Kryscio R., and Thomas M.V. Salivary biomarkers of existing periodontal disease: a cross-sectional study. J Am Dent Assoc 137 (2006) 322-329
16. Griffiths G.S. Formation, collection and significance of gingival crevice fluid. Periodontol 2000 31 (2003) 32-42
17. Griffiths G.S., Sterne J.A., Wilton J.M., Eaton K.A., and Johnson N.W. Associations between volume and flow rate of gingival crevicular fluid and clinical assessments of gingival inflammation in a population of British male adolescents. J Clin Periodontol 19 (1992) 464-470
18. Lundquist J., and Josefsson J.O. Sensitive method for determination of peroxidase activity in tissue by means of coupled oxidation reaction. Anal Biochem 41 (1971) 567-577
19. Herzog V., and Fahimi H.D. A new sensitive colorimetric assay for peroxidase using 3, 3′-diaminobenzidine as hydrogen donor. Anal Biochem 55 (1973) 554-562
20. Pruitt K.M., Kamau D.N., Miller K., Mansson-Rahemtulla B., and Rahemtulla F. Quantitative, standardized assays for determining the concentrations of bovine lactoperoxidase, human salivary peroxidase, and human myeloperoxidase. Anal Biochem 191 (1990) 278-286
21. Sakamoto W., Fukuda H., and Nishikaze O. Kininogen and kallikrein in saliva of periodontally-diseased subjects. J Dent Res 60 (1981) 6-9
22. Malle E., Marsche G., Arnhold J., and Davies M.J. Modification of low-density lipoprotein by myeloperoxidase-derived oxidants and reagent hypochlorus acid. Biochim Biophys Acta 1761 (2006) 392-415
23. Hampton M.B., Kettle A.J., and Winterbourn C.C. Inside the neutrophil phagosome: oxidants, myeloperoxidase, and bacterial killing. Blood 92 (1998) 3007-3017
24. Uitto V.J., Overall C.M., and McCulloch C. Proteolytic host cell enzymes in gingival crevice fluid. Periodontol 2000 31 (2003) 77-104
25. Frank T., Kohnen S., Deby-Dupont G., Grulke S., Deby C., and Serteyn D. A specific method for measurement of equine active myeloperoxidase in biological samples and in vitro tests. J Vet Diagn Invest 18 (2006) 326-334
26. Capeillere-Blandin C. Oxidation of guaiacol by myeloperoxidase: a two-electron-oxidized guaiacol transient species as a mediator of NADH oxidation. Biochem J 336 (1998) 395-404
27. Lindgren B.O. Dehydrogenation of phenols. 2. Dehydrogenation polymers from guaiacol. Acta Chem Scand 14 (1960) 2089-2096
28. Tiruppathi C., Naqvi T., Wu Y., Vogel S.M., Minshall R.D., and Malik A.B. Albumin mediates the transcytosis of myeloperoxidase by means of caveolae in endothelial cells. Proc Nat Acad sci U S A 101 (2004) 7699-7704
29. Salavej P., Spalteholz H., and Arnhold J. Modification of amino acid residues in human serum albumin by myeloperoxidase. Free Radic Biol Med 40 (2006) 516-525
30. Bozeman P.M., Learn D.B., and Thomas E.L. Inhibition of the human leukocyte enzymes myeloperoxidase and eosinophil peroxidase by dapsone. Biochem Pharmacol 44 (1992) 553-563
31. Thomas E.L., Jefferson M.M., Joyner R.E., Cook G.S., and King C.C. Leukocyte myeloperoxidase and salivary lactoperoxidase: identification and quantitation in human mixed saliva. J Dent Res 73 (1994) 544-555
32. Herr A.E., Hatch A.V., Throckmorton D.J., Tran H.M., Brennan J.S., Giannobile W.V., et al. Microfluidic immunoassays as rapid saliva-based clinical diagnostics. Proc Natl Acad Sci U S A 104 (2007) 5268-5273