Difficult-to-identify bacteria: How use of 16S rDNA PCR and gene sequencing can help

British Journal of Biomedical Science

Volumn 65, Issue 1, 2008, Pages 33-36

  Xu, J ^a,b,c   Stanley, T ^a   Millar, B C ^a   McClurg, R B ^a   Shaw, A ^a   Crothers, L ^a   Goldsmith, C E ^a   Rooney, P J ^a   Loughrey, A ^a   Murphy, P G ^a,d   Dooley, J S G ^c   Moore, John E ^a  

^a BELFAST CITY HOSPITAL   (United Kingdom)

^b XI'AN JIAOTONG UNIVERSITY   (China)

^c UNIVERSITY OF ULSTER   (United Kingdom)

^d ADELAIDE AND MEATH HOSPITAL   (Ireland)

Author keywords

[No Author keywords available]

Indexed keywords

RIBOSOME DNA;

ARTICLE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; CYSTIC FIBROSIS; GENE SEQUENCE; HUMAN; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; SEQUENCE ANALYSIS;

EID: 41749113069     PISSN: 09674845     EISSN: None     Source Type: Journal    
DOI: 10.1080/09674845.2008.11978106     Document Type: Article

References (6)

1. Xu J, Smyth CL, Buchanan JA et al. Employment of 16S rDNA gene sequencing techniques to identify culturable environmental eubacteria in a tertiary referral hospital. J Hosp Infect 2004; 57: 52-8.
2. Moissenet D, Bingen E, Arlet G, Vu-Thien H. Use of 16S rRNA gene sequencing for identification of "Pseudomonas-like" isolates from sputum of patients with cystic fibrosis (in French). Pathol Biol (Paris) 2005; 53 (8-9): 500-2.
3. Woese CR. Bacterial evolution. Microbiol Rev 1987; 51: 221-71.
4. Lau SK, Woo PC, Fung AM, Chan KM, Woo GK, Yuen KY. Anaerobic, non-sporulating, Gram-positive bacilli bacteraemia characterized by 16S rRNA gene sequencing. J Med Microbiol 2004; 53: 1247-53.
5. Woo PC, Lau SK, Teng JL, Yuen KY. Current status and future directions for Laribacter hongkongensis, a novel bacterium associated with gastroenteritis and travellers' diarrhoea. Curr Opin Infect Dis 2005; 18: 413-9.
6. Millar BC, Xu J, Moore JE. Risk assessment models and contamination management: implications for broad-range ribosomal DNA PCR as a diagnostic tool in medical bacteriology. J Clin Microbiol 2002; 40: 1575-80.