Quantification of cardiac fibrosis by colour-subtractive computer-assisted image analysis

Clinical and Experimental Pharmacology and Physiology

Volumn 35, Issue 5-6, 2008, Pages 679-686

  Gaspard, Gerard J ^a   Pasumarthi, Kishore B S ^a  

^a DALHOUSIE UNIVERSITY   (Canada)

Author keywords

Cardiac fibrosis; Cardiac hypertrophy; Color subtractive image analysis; Fibrosis quantification; Isoproterenol

Indexed keywords

ANTIFIBROTIC AGENT; BETA 1 ADRENERGIC RECEPTOR; CATECHOLAMINE; ISOPRENALINE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CARDIOVASCULAR DISEASE; COLOR SUBTRACTIVE COMPUTER ASSISTED IMAGE ANALYSIS; COMPUTER SYSTEM; CONTROLLED STUDY; DISEASE SEVERITY; DOG; GENE EXPRESSION; HEART MUSCLE FIBROSIS; HEART VENTRICLE HYPERTROPHY; IMAGE ANALYSIS; MALE; MOUSE; NONHUMAN; QUANTITATIVE ANALYSIS; RELIABILITY;

ANIMALS; COLOR; FIBROSIS; HEART DISEASES; IMAGE PROCESSING, COMPUTER-ASSISTED; ISOPROTERENOL; MALE; MICE; RATS; RATS, WISTAR; SOFTWARE; USER-COMPUTER INTERFACE;

EID: 41749112208     PISSN: 03051870     EISSN: 14401681     Source Type: Journal    
DOI: 10.1111/j.1440-1681.2008.04930.x     Document Type: Article

References (41)

1. Brown RD, Ambler SK, Mitchell MD, Long CS. The cardiac fibroblast: Therapeutic target in myocardial remodeling and failure. Annu. Rev. Pharmacol. Toxicol. 2005 45 : 657 87.
2. Virag JI, Murry CE. Myofibroblast and endothelial cell proliferation during murine myocardial infarct repair. Am. J. Pathol. 2003 163 : 2433 40.
3. Eghbali M, Weber KT. Collagen and the myocardium: Fibrillar structure, biosynthesis and degradation in relation to hypertrophy and its regression. Mol. Cell. Biochem. 1990 96 : 1 14.
4. Berk BC, Fujiwara K, Lehoux S. ECM remodeling in hypertensive heart disease. J. Clin. Invest. 2007 117 : 568 75.
5. Jalil JE, Janicki JS, Pick R, Abrahams C, Weber KT. Fibrosis-induced reduction of endomyocardium in the rat after isoproterenol treatment. Circ. Res. 1989 65 : 258 64.
6. Ohsato K, Shimizu M, Sugihara N, Konishi K, Takeda R. Histopathological factors related to diastolic function in myocardial hypertrophy. Jpn Circ. J. 1992 56 : 325 33.
7. Tabib A, Loire R, Chalabreysse L et al. Circumstances of death and gross and microscopic observations in a series of 200 cases of sudden death associated with arrhythmogenic right ventricular cardiomyopathy and/or dysplasia. Circulation 2003 108 : 3000 5.
8. Klappacher G, Franzen P, Haab D et al. Measuring extracellular matrix turnover in the serum of patients with idiopathic or ischemic dilated cardiomyopathy and impact on diagnosis and prognosis. Am. J. Cardiol. 1995 75 : 913 18.
9. Shirani J, Pick R, Roberts WC, Maron BJ. Morphology and significance of the left ventricular collagen network in young patients with hypertrophic cardiomyopathy and sudden cardiac death. J. Am. Coll. Cardiol. 2000 35 : 36 44.
10. Querejeta R, Varo N, Lopez B et al. Serum carboxy-terminal propeptide of procollagen type I is a marker of myocardial fibrosis in hypertensive heart disease. Circulation 2000 101 : 1729 35.
11. Lopez B, Gonzalez A, Varo N, Laviades C, Querejeta R, Diez J. Biochemical assessment of myocardial fibrosis in hypertensive heart disease. Hypertension 2001 38 : 1222 6.
12. Lindy S, Turto H, Uitto J. Protocollagen proline hydroxylase activity in rat heart during experimental cardiac hypertrophy. Circ. Res. 1972 30 : 205 9.
13. Turto H. Collagen metabolism in experimental cardiac hypertrophy in the rat and the effect of digitoxin treatment. Cardiovasc. Res. 1977 11 : 358 66.
14. Faulx MD, Ernsberger P, Vatner D et al. Strain-dependent beta-adrenergic receptor function influences myocardial responses to isoproterenol stimulation in mice. Am. J. Physiol. Heart Circ. Physiol. 2005 289 : H30 6.
15. Whittaker P, Kloner RA, Boughner DR, Pickering JG. Quantitative assessment of myocardial collagen with picrosirius red staining and circularly polarized light. Basic Res. Cardiol. 1994 89 : 397 410.
16. Benjamin IJ, Jalil JE, Tan LB, Cho K, Weber KT, Clark WA. Isoproterenol-induced myocardial fibrosis in relation to myocyte necrosis. Circ. Res. 1989 65 : 657 70.
17. Young M, Fullerton M, Dilley R, Funder J. Mineralocorticoids, hypertension, and cardiac fibrosis. J. Clin. Invest. 1994 93 : 2578 83.
18. Young M, Head G, Funder J. Determinants of cardiac fibrosis in experimental hypermineralocorticoid states. Am. J. Physiol. 1995 269 : E657 62.
19. Eghbali M, Robinson TF, Seifter S, Blumenfeld OO. Collagen accumulation in heart ventricles as a function of growth and aging. Cardiovasc. Res. 1989 23 : 723 9.
20. Vasiljevic JD, Popovic ZB, Otasevic P et al. Myocardial fibrosis assessment by semiquantitative, point-counting and computer-based methods in patients with heart muscle disease: A comparative study. Histopathology 2001 38 : 338 43.
21. Underwood RA, Gibran NS, Muffley LA, Usui ML, Olerud JE. Color subtractive-computer-assisted image analysis for quantification of cutaneous nerves in a diabetic mouse model. J. Histochem. Cytochem. 2001 49 : 1285 91.
22. Soonpaa MH, Field LJ. Assessment of cardiomyocyte DNA synthesis during hypertrophy in adult mice. Am. J. Physiol. 1994 266 : H1439 45.
23. Kudej RK, Iwase M, Uechi M et al. Effects of chronic beta-adrenergic receptor stimulation in mice. J. Mol. Cell. Cardiol. 1997 29 : 2735 46.
24. Brouri F, Findji L, Mediani O et al. Toxic cardiac effects of catecholamines: Role of beta-adrenoceptor downregulation. Eur. J. Pharmacol. 2002 456 : 69 75.
25. Oudit GY, Crackower MA, Eriksson U et al. Phosphoinositide 3-kinase gamma-deficient mice are protected from isoproterenol-induced heart failure. Circulation 2003 108 : 2147 52.
26. Murad N, Tucci PJ. Isoproterenol-induced hypertrophy may result in distinct left ventricular changes. Clin. Exp. Pharmacol. Physiol. 2000 27 : 352 7.
27. Brouri F, Hanoun N, Mediani O et al. Blockade of beta 1- and desensitization of beta 2-adrenoceptors reduce isoprenaline-induced cardiac fibrosis. Eur. J. Pharmacol. 2004 485 : 227 34.
28. Mori H, Ishikawa S, Kojima S et al. Increased responsiveness of left ventricular apical myocardium to adrenergic stimuli. Cardiovasc. Res. 1993 27 : 192 8.
29. Goldspink DF, Burniston JG, Ellison GM, Clark WA, Tan LB. Catecholamine-induced apoptosis and necrosis in cardiac and skeletal myocytes of the rat in vivo: The same or separate death pathways? Exp. Physiol. 2004 89 : 407 16.
30. Burniston JG, Tan LB, Goldspink DF. beta2-Adrenergic receptor stimulation in vivo induces apoptosis in the rat heart and soleus muscle. J. Appl. Physiol. 2005 98 : 1379 86.
31. Upsher ME, Weiss HR. Heterogeneous distribution of beta adrenoceptors in the dog left ventricle. J. Mol. Cell. Cardiol. 1986 18 : 657 60.
32. 1 receptor subtype as a cardiac target of aldosterone: Role in aldosterone-salt-induced fibrosis. Hypertension 1999 33 : 981 6.
33. Rocha R, Rudolph AE, Frierdich GE et al. Aldosterone induces a vascular inflammatory phenotype in the rat heart. Am. J. Physiol. Heart Circ. Physiol. 2002 283 : H1802 10.
34. Ikeuchi M, Tsutsui H, Shiomi T et al. Inhibition of TGF-beta signaling exacerbates early cardiac dysfunction but prevents late remodeling after infarction. Cardiovasc. Res. 2004 64 : 526 35.
35. Dean RG, Balding LC, Candido R et al. Connective tissue growth factor and cardiac fibrosis after myocardial infarction. J. Histochem. Cytochem. 2005 53 : 1245 56.
36. Boerma M, Wang J, Wondergem J et al. Influence of mast cells on structural and functional manifestations of radiation-induced heart disease. Cancer Res. 2005 65 : 3100 7.
37. Falcon BL, Stewart JM, Bourassa E et al. Angiotensin II type 2 receptor gene transfer elicits cardioprotective effects in an angiotensin II infusion rat model of hypertension. Physiol. Genomics 2004 19 : 255 61.
38. Delaughter MC, Taffet GE, Fiorotto ML, Entman ML, Schwartz RJ. Local insulin-like growth factor I expression induces physiologic, then pathologic, cardiac hypertrophy in transgenic mice. FASEB J. 1999 13 : 1923 9.
39. Beggah AT, Escoubet B, Puttini S et al. Reversible cardiac fibrosis and heart failure induced by conditional expression of an antisense mRNA of the mineralocorticoid receptor in cardiomyocytes. Proc. Natl Acad. Sci. USA 2002 99 : 7160 5.
40. Pedersen B, Holscher T, Sato Y, Pawlinski R, Mackman N. A balance between tissue factor and tissue factor pathway inhibitor is required for embryonic development and hemostasis in adult mice. Blood 2005 105 : 2777 82.
41. Mekontso-Dessap A, Brouri F, Pascal O et al. Deficiency of the 5-hydroxytryptamine transporter gene leads to cardiac fibrosis and valvulopathy in mice. Circulation 2006 113 : 81 9.