Reversible compartmentalization of de novo purine biosynthetic complexes in living cells

Science

Volumn 320, Issue 5872, 2008, Pages 103-106

  An, Songon ^a   Kumar, Ravindra ^a   Sheets, Erin D ^a   Benkovic, Stephen J ^a  

^a PENNSYLVANIA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME; PURINE;

CATALYSIS; COMPARTMENTALIZATION; CYTOPLASM; ENZYME ACTIVITY; EUKARYOTE; FLUORESCENCE; MICROSCOPY; PROTEIN;

ARTICLE; BIOSYNTHESIS; CELL COMPARTMENTALIZATION; CULTURE MEDIUM; CYTOPLASM; FLUORESCENCE MICROSCOPY; HELA CELL; HUMAN; HUMAN CELL; PRIORITY JOURNAL;

AZASERINE; BINDING SITES; CARBON-NITROGEN LIGASES; CARBON-NITROGEN LIGASES WITH GLUTAMINE AS AMIDE-N-DONOR; CELL COMPARTMENTATION; CELL LINE; CELL LINE, TUMOR; CULTURE MEDIA; CYTOPLASM; FLUORESCENT ANTIBODY TECHNIQUE; HELA CELLS; HUMANS; HYPOXANTHINE; MICROSCOPY, FLUORESCENCE; MULTIENZYME COMPLEXES; PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE; PURINES; RECOMBINANT FUSION PROTEINS; TRANSFECTION;

EUKARYOTA;

EID: 41749092312     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1152241     Document Type: Article

References (23)

1. C. Stasolla, R. Katahira, T. A. Thorpe, H. Ashihara, J. Plant Physiol. 160, 1271 (2003).
2. R. Zrenner, M. Stitt, U. Sonnewald, R. Boldt, Annu. Rev. Plant Biol. 57, 805 (2006).
3. C. Z. Constantine et al., Biochemistry 45, 8193 (2006).
4. C. A. Caperelli, P. A. Benkovic, G. Chettur, S. J. Benkovic, J. Biol. Chem. 255, 1885 (1980).
5. G. K. Smith, W. T. Mueller, G. F. Wasserman, W. D. Taylor, S. J. Benkovic, Biochemistry 19, 4313 (1980).
6. J. Rudolph, J. Stubbe, Biochemistry 34, 2241 (1995).
7. L. T. Gooljarsingh, J. Ramcharan, S. Gilroy, S. J. Benkovic, Proc. Natl. Acad. Sci. U.S.A. 98, 6565 (2001).
8. M. A. Becker, M. Kim, J. Biol. Chem. 262, 14531 (1987).
9. T. Yamaoka et al., J. Biol. Chem. 272, 17719 (1997).
10. T. Yamaoka et al., J. Biol. Chem. 276, 21285 (2001).
11. Materials and methods are available as supporting material on Science Online.
12. G. F. Wasserman, W. T. Mueller, S. J. Benkovic, W. S. Liao, J. Taylor, Biochemistry 23, 6704 (1984).
13. M. Howarth, K. Takao, Y. Hayashi, A. Y. Ting, Proc. Natl. Acad. Sci. U.S.A. 102, 7583 (2005).
14. M. Kondo et al., J. Biochem. (Tokyo) 128, 57 (2000).
15. H. Iwahana et al., Biochim. Biophys. Acta 1261, 369 (1995).
16. L. F. Thompson, R. C. Willis, J. W. Stoop, J. E. Seegmiller, Proc. Natl. Acad. Sci. U.S.A. 75, 3722 (1978).
17. M. A. Becker, M. J. Losman, M. Kim, J. Biol. Chem. 262, 5596 (1987).
18. S. X. Li et al., J. Mol. Biol. 366, 1603 (2007).
19. L. Pagliaro, D. L. Taylor, J. Cell Biol. 107, 981 (1988).
20. M. Pozuelo Rubio et al., Biochem. J. 379, 395 (2004).
21. M. E. Campanella, H. Chu, P. S. Low, Proc. Natl. Acad. Sci. U.S.A. 102, 2402 (2005).
22. F. R. Opperdoes, P. Borst, FEBS Lett. 80, 360 (1977).
23. We thank M. Kyoung (Pennsylvania State University) for valuable suggestions on data collection, imaging analysis, and statistics; E. Kunze (Pennsylvania State University) for assistance with confocal laser-scanning microscopy; A. A. Heikal (Pennsylvania State University) for sharing HTB-125 and -126 cell lines; and R. Y. Tsien (University of California, San Diego) for providing the pRSET_mOrange plasmid as a gift. This work was supported, in part, by Pennsylvania State University and the Center for Optical Technologies (E.D.S.). Additional acknowledgment is made to the Donors of the American Chemical Society Petroleum Research Fund, for partial support of this research (E.D.S.).