Measuring intracellular enzyme concentrations assessing the effect of oxidative stress on the amount of glyoxalase I

Biochemistry and Molecular Biology Education

Volumn 36, Issue 2, 2008, Pages 135-138

  Vicente Miranda, Hugo ^a,b   Ferreira, Antonio E N ^a   Quintas, Alexandre ^a,b   Cordeiro, Carlos ^a   Freire, Ana Ponces ^a  

^a UNIVERSITY OF LISBON   (Portugal)

^b INSTITUTO SUPERIOR DE CIÊNCIAS DA SAÚDE EGAS MONIZ   (Portugal)

Author keywords

Enzymes and catalysis; Laboratory exercises; Problem based learning

Indexed keywords

EID: 41549151617     PISSN: 14708175     EISSN: None     Source Type: Journal    
DOI: 10.1002/bmb.20166     Document Type: Article

References (15)

1. A. Cornish-Bowden, M. L. Cardenas (2005) Systems biology may work when we learn to understand the parts in terms of the whole, Biochem. Soc. Trans. 33, 516-519.
2. J. J. Aragon, J. E. Feliu, R. A. Frenkel, A. Sols (1980) Permeabilization of animal cells for kinetic studies of intracellular enzymes: In situ behavior of the glycolytic enzymes of erythrocytes, Proc. Natl. Acad. Sci. USA 77, 6324-6328.
3. C. Cordeiro, A. P. Freire (1995) Digitonin permeabilization of Saccharomyces cerevisiae cells for in situ enzyme assay, Anal. Biochem. 229, 145-148.
4. A. Cornish-Bowden (2004) Fundamentals of Enzyme Kinetics, Portland Press Ltd., London.
5. H. V. Miranda, A. E. Ferreira, C. Cordeiro, A. P. Freire (2006) Kinetic assay for measurement of enzyme concentration in situ, Anal. Biochem. 354, 148-150.
6. J. R Richard (1993) Mechanism for the formation of methylglyoxal from triosephosphates, Biochem. Soc. Trans. 21, 549-553.
7. P. J. Thornalley (1990) The glyoxalase system: New developments towards functional characterization of a metabolic pathway fundamental to biological life, Biochem. J. 269, 1-11.
8. D. L. Vander Jagt, L. A. Hunsaker (1993) Substrate specificity of reduced and oxidized forms of human aldose reductase, Adv. Exp. Med. Biol. 328, 279-288.
9. P. J. Thornalley (1988) Modification of the glyoxalase system in human red blood cells by glucose in vitro, Biochem. J. 254, 751-755.
10. C. A. Cordeiro, A. P. Freire (1994) Protein determination in permeabilized yeast cells using the Coomassie brilliant blue dye binding assay, Anal. Biochem. 223, 321-323.
11. R. A. Gomes, M. S. Silva, H. V. Miranda, A. E. Ferreira, C. A. Cordeiro, A. P. Freire (2005) Protein glycation in Saccharomyces cerevisiae. Argpyrimidine formation and methylglyoxal catabolism, FEBS J. 272, 4521-4531.
12. R. Eisenthal, A. Cornish-Bowden (1974) The direct linear plot. A new graphical procedure for estimating enzyme kinetic parameters, Biochem. J. 139, 715-720.
13. J. S. Easterby (1992) HyperFit-Hyperbolic Regression Analysis of Enzyme Kinetic Data, Hyper.exe, Version 1.0 (Shareware program), Department of Biochemistry, University of Liverpool, Liverpool, UK.
14. R Moradas-Ferreira, V. Costa, P. Piper, W. Mager (1996) The molecular defences against reactive oxygen species in yeast, Mol. Microbiol. 19, 651-658.
15. X. D. Liu, P. C. Liu, N. Santoro, D. J. Thiele (1997) Conservation of a stress response: Human heat shock transcription factors functionally substitute for yeast HSF, EMBO J. 16, 6466-6477.