Flagellin and outer surface proteins from Borrelia burgdorferi are not glycosylated

Journal of Bacteriology

Volumn 190, Issue 7, 2008, Pages 2619-2623

  Štěrba, Ján ^a,b   Vancová, Marie ^a,b   Rudenko, Nataliia ^a,b   Golovchenko, Maryna ^a,b   Tremblay, Tammy Lynn ^c   Kelly, John F ^c   MacKenzie, C Roger ^c   Logan, Susan M ^c   Grubhoffer, Libor ^a,b  

^a UNIVERSITY OF SOUTH BOHEMIA   (Czech Republic)

^b INSTITUTE OF PARASITOLOGY   (Czech Republic)

^c NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; FLAGELLAR PROTEIN FLAA; FLAGELLAR PROTEIN FLAB; FLAGELLIN; GLYCOPROTEIN; OUTER SURFACE PROTEIN A; OUTER SURFACE PROTEIN B; UNCLASSIFIED DRUG;

ARTICLE; BORRELIA BURGDORFERI; CULTURE MEDIUM; GLYCOSYLATION; NONHUMAN; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; ANTIGENS, BACTERIAL; ANTIGENS, SURFACE; BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL VACCINES; BORRELIA BURGDORFERI; CRYOELECTRON MICROSCOPY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLAGELLIN; GLYCOSYLATION; LIPOPROTEINS; MOLECULAR SEQUENCE DATA; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

BORRELIA BURGDORFERI;

EID: 41549138353     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.01885-07     Document Type: Article

References (21)

1. Akin, E., G. L. McHugh, R. A. Flavell, E. Fikrig, and A. C. Steere. 1999. The immunoglobulin G (IgG) antibody response to OspA and OspB correlates with severe and prolonged Lyme arthritis, and the IgG response to P35 correlates with mild and brief arthritis. Infect. Immun. 67:173-181.
2. Brahamsha, B., and E. P. Greenberg. 1988. Biochemical and cytological analysis of the complex periplasmic flagella from Spirochaeta aurantia. J. Bacteriol. 170:4023-4032.
3. Brandt, M. E., B. S. Riley, J. D. Radolf, and M. V. Norgard. 1990. Immunogenic integral membrane proteins of Borrelia burgdorferi are lipoproteins. Infect. Immun. 58:983-991.
4. Brooks, C. S., P. S. Hefty, S. E. Jolliff, and D. R. Akins. 2003. Global analysis of Borrelia burgdorferi genes regulated by mammalian host-specific signals. Infect. Immun. 71:3371-3383.
5. Charon, N. W., and S. F. Goldstein. 2002. Genetics of motility and chemotaxis of a fascinating group of bacteria: the spirochetes. Annu. Rev. Genet. 36:47-73.
6. Coburn, J., J. R. Fischer, and J. M. Leong. 2005. Solving a sticky problem: new genetic approaches to host cell adhesion by the Lyme disease spirochete. Mol. Microbiol. 57:1182-1195.
7. Coleman, J. L., and J. L. Benach. 1989. Identification and characterization of an endoflagellar antigen of Borrelia burgdorferi. J. Clin. Investig. 84:322-330.
8. Ge, Y., C. Li, L. Coram, C. A. Slaughter, and N. W. Charon. 1998. Structure and expression of the FlaA periplasmic flagellar protein of Borrelia burgdorferi. J. Bacteriol. 180:2418-2425.
9. Hulínská, D., P. Volf, and L. Grubhoffer. 1992. Study of Borrelia burgdorferi glycoproteins and surface carbohydrates by lectin conjugates. Zentralbl. Bakteriol. 276:473-480.
10. Kellenberger, C., T. L. Hendrikson, and B. Imperiali. 1997. Structural and functional analysis of peptidyl oligosaccharyl transferase inhibitors. Biochemistry 36:12554-12559.
11. Li, Z., F. Dumas, D. Dubreuil, and M. Jacques. 1993. A species-specific periplasmic flagellar protein of Serpulina (Treponema) hyodysenteriae. J. Bacteriol. 175:8000-8007.
12. Neelekanta, G., X. Li, U. Pal, X. Liu, D. S. Beck, K. DePonte, D. Fish, F. S. Kantor, and E. Fikrig. 2007. Outer surface protein B is critical for Borrelia burgdorferi adherence and survival within Ixodes ticks. PLoS Pathog. 3:1-11.
13. Nita-Lazar, M., M. Wacker, B. Schegg, S. Amber, and M. Aebi. 2005. The N-X-S/T consensus sequence is required but not sufficient for bacterial N-linked protein glycosylation. Glycobiology 15:361-367.
14. Pal, U., X. Li, T. Wang, R. R. Montgomery, N. Ramamoorthi, A. M. Desilva, F. Bao, X. Yang, M. Pypaert, D. Pradhan, F. S. Kantor, S. Telford, J. F. Anderson, and E. Fikrig. 2004. TROSPA, an Ixodes scapularis receptor for Borrelia burgdorferi. Cell 119:457-468.
15. Sambri, V., C. Stefanelli, and R. Cevenini. 1992. Detection of glycoproteins in Borrelia burgdorferi. Arch. Microbiol. 157:205-208.
16. Shoberg, R. J., and D. D. Thomas. 1995. Borrelia burgdorferi vesicle production occurs via a mechanism independent of immunoglobulin M involvement. Infect. Immun. 63:4857-4861.
17. Simon, M. I., S. U. Emerson, J. H. Shaper, P. D. Bernard, and A. N. Glazer. 1977. Classification of Bacillus subtilis flagellins. J. Bacteriol. 130:200-204.
18. Stoitsová, T. R., L. Grubhoffer, and J. Nebesá rová. 2003. Exposed and hidden lectin-binding epitopes at the surface of Borrelia burgdorferi. Folia Microbiol. 48:654-658.
19. Szymanski, C. M., and B. W. Wren. 2005. Protein glycosylation in bacterial mucosal pathogens. Nat. Rev. Microbiol. 3:225-237.
20. Vancova, M., J. Nebesarova, and L. Grubhoffer. 2005. Lectin-binding characteristics of a Lyme borreliosis spirochete, Borrelia burgdorferi sensu stricto. Folia Microbiol. (Prague) 50:229-238.
21. Wyss, C. 1998. Flagellins, but not endoflagellar sheath proteins, of Treponema pallidum and of pathogen-related oral spirochetes are glycosylated. Infect. Immun. 66:5751-5754.