New pSC101-derivative cloning vectors with elevated copy numbers

Plasmid

Volumn 59, Issue 3, 2008, Pages 193-201

  Peterson, James ^a   Phillips, Gregory J ^a  

^a IOWA STATE UNIVERSITY   (United States)

Author keywords

Copy number mutants; Gene expression; Plasmid incompatibility

Indexed keywords

AMPICILLIN; CHLORAMPHENICOL; F FACTOR; GLUTAMIC ACID; KANAMYCIN; NUCLEIC ACID BINDING PROTEIN; PLASMID VECTOR; PROTEIN REPA; PROTEIN REPE; SIGNAL RECOGNITION PARTICLE; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; ARTICLE; CLONING VECTOR; CONTROLLED STUDY; CRYSTAL STRUCTURE; DIMERIZATION; ESCHERICHIA COLI; GENE EXPRESSION; GENE INSERTION; GENE MUTATION; GENE REPRESSION; NONHUMAN; PLASMID; PLASMID BR322; PLASMID JPA12; PLASMID JPA13; PLASMID SC101; PLASMID WSK30; PROTEIN ISOLATION; REPLICON; TEMPERATURE SENSITIVE MUTANT; WILD TYPE;

AMPICILLIN; BACTERIAL PROTEINS; CHLORAMPHENICOL; CLONING, MOLECULAR; DNA HELICASES; DNA-BINDING PROTEINS; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC TECHNIQUES; GENETIC VECTORS; GENOTYPE; KANAMYCIN; MUTAGENESIS, SITE-DIRECTED; MUTATION; PLASMIDS; REPRESSOR PROTEINS; TRANS-ACTIVATORS;

ESCHERICHIA COLI;

EID: 41549090370     PISSN: 0147619X     EISSN: 10959890     Source Type: Journal    
DOI: 10.1016/j.plasmid.2008.01.004     Document Type: Article

References (35)

1. 3 molecular weight plasmid-encoded protein is required for replication and copy number control in the plasmid pSC101 and its temperature-sensitive derivative pHS1. J. Mol. Biol. 175 (1984) 331-347
2. Cabello F., Timmis K., and Cohen S.N. Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons. Nature 259 (1976) 285-290
3. Cesareni G., Helmer-Citterich M., and Castagnoli L. Control of ColE1 plasmid replication by antisense RNA. Trends Genet. 7 (1991) 230-235
4. Chang A.C., and Cohen S.N. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the p15A cryptic miniplasmid. J. Bacteriol. 134 (1978) 1141-1156
5. Chattoraj D.K. Control of plasmid DNA replication by iterons: no longer paradoxical. Mol. Microbiol. 37 (2000) 467-476
6. Churchward G., Linder P., and Caro L. The nucleotide sequence of replication and maintenance functions encoded by plasmid pSC101. Nucleic Acids Res. 11 (1983) 5645-5659
7. Cohen S.N., Chang A.C., Boyer H.W., and Helling R.B. Construction of biologically functional bacterial plasmids in vitro. Proc. Natl. Acad. Sci. USA 70 (1973) 3240-3244
8. Diaz-Lopez T., Lages-Gonzalo M., Serrano-Lopez A., Alfonso C., Rivas G., Diaz-Orejas D., and Girldo R. Structural changes in Rep, plasmid replication initiator, upon binding to origin DNA. J. Biol. Chem. 278 (2003) 18606-18616
9. Furuno S., Watanabe-Murakami Y., Takebe-Suzuki N., and Yamaguchi K. Negative control of plasmid pSC101 replication by increased concentrations of both initiator protein and iterons. J. Gen. Appl. Microbiol. 46 (2000) 29-37
10. Hashimoto-Gotoh T., Yamaguchi M., Yasojima K., Tsujimura A., Wakabayashi Y., and Watanabe Y. A set of temperature sensitive-replication/-segregation and temperature resistant plasmid vectors with different copy numbers and in an isogenic background (chloramphenicol, kanamycin, lacZ, repA, par, polA). Gene 241 (2000) 185-191
11. Hasnain S., and Thomas C.M. Construction of a novel gene bank of Bacillus subtilis using a low copy number vector in Escherichia coli. J. Gen. Microbiol. 132 (1986) 1863-1874
12. Hasunuma K., and Sekiguchi M. Replication of plasmid pSC101 in Escherichia coli K12: requirement for dnaA function. Mol. Gen. Genet. 154 (1977) 223-225
13. Hershfield V., Boyer H.W., Yanofsky C., Lovett M.A., and Helinski D.R. Plasmid ColE1 as a molecular vehicle for cloning and amplification of DNA. Proc. Natl. Acad. Sci. USA 71 (1974) 3455-3459
14. Hiszczynska-Sawicka E., and Kur J. Effect of Escherichia coli IHF mutations on plasmid p15A copy number. Plasmid 38 (1997) 174-179
15. Hoang T.T., Ma Y., Stern R.J., McNeil M.R., and Schweizer H.P. Construction and use of low-copy number T7 expression vectors for purification of problem proteins: purification of Mycobacterium tuberculosis RmlD and Pseudomonas aeruginosa LasI and RhlI proteins, and functional analysis of purified RhlI. Gene 237 (1999) 361-371
16. Lee C., Kim J., Shin S.G., and Hwang S. Absolute and relative QPCR quantification of plasmid copy number in Escherichia coli. J. Biotechnol. 123 (2006) 273-280
17. Lerner C.G., and Inouye M. Low copy number plasmids for regulated low-level expression of cloned genes in Escherichia coli with blue/white insert screening capability. Nucleic Acids Res. 18 (1990) 4631
18. Lin-Chao S., and Bremer H. Effect of the bacterial growth rate on replication control of plasmid pBR322 in Escherichia coli. Mol. Gen. Genet. 203 (1986) 143-149
19. Linder P., Churchward G., Xia G.X., Yu Y.Y., and Caro L. An essential replication gene, repA, of plasmid pSC101 is autoregulated. J. Mol. Biol. 181 (1985) 383-393
20. Lu Q. Plasmid vectors for gene cloning and expression. In: Funnell B.E., and Phillips G.J. (Eds). Plasmid Biology (2004), ASM Press, Washington, DC
21. Park S.K., Jiang F., Dalbey R.E., and Phillips G.J. Functional analysis of the signal recognition particle in Escherichia coli by characterization of a temperature-sensitive ffh mutant. J. Bacteriol. 184 (2002) 2642-2653
22. Phillips G.J. New cloning vectors with temperature-sensitive replication. Plasmid 41 (1999) 78-81
23. Phillips G.J., Park S.K., and Huber D. High copy number plasmids compatible with commonly used cloning vectors. Biotechniques 28 (2000) 400-406
24. Polisky B. ColE1 replication control circuitry: sense from antisense. Cell 23 (1988) 929-932
25. Sharma S., Sathyanarayana K., Bird J.G., Hoskins J.R., Lee B., and Wickner S. Plasmid P1 Rep is homologous to the F plasmid RepE class of initiators. J. Biol. Chem. 279 (2004) 6027-6034
26. Stemmer W.P., and Morris S.K. Enzymatic inverse PCR: a restriction site independent, single-fragment method for high-efficiency, site-directed mutagenesis. Biotechniques 13 (1992) 214-220
27. Stoker N.G., Fairweather N.F., and Spratt B.G. Versatile low-copy-number plasmid vectors for cloning in Escherichia coli. Gene 18 (1982) 335-341
28. Takeshita S., Sato M., Toba M., Masahashi W., and Hashimoto-Gotoh T. High-copy-number and low-copy-number plasmid vectors for lacZ alpha-complementation and chloramphenicol- or kanamycin-resistance selection. Gene (1987) 63-74
29. Tucker W.T., Miller C.A., and Cohen S.N. Structural and functional analysis of the par region of the pSC101 plasmid. Cell 38 (1984) 191-201
30. Vocke C., and Bastia D. DNA-protein interaction at the origin of DNA replication of the plasmid pSC101. Cell 35 (1983) 495-502
31. Wang R.F., and Kushner S.R. Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli. Gene 100 (1991) 195-199
32. Weitao T., Dasgupta S., and Nordström K. Plasmid R1 is present as clusters in the cells of Escherichia coli. Plasmid 43 (2000) 200-204
33. Whelan J.A., Russel N.B., and Whelan M.A. A method for the absolute quantification of cDNA using real time PCR. J. Immunol. Meth. 278 (2003) 261-269
34. Xia G., Manen D., Yu Y., and Caro L. In vivo and in vitro studies of a copy number mutation of the RepA replication protein of plasmid pSC101. J. Bacteriol. 175 (1993) 4165-4175
35. Xia G.-X., Manen D., Goebel T., Linder P., Churchward G., and Caro L. A copy-number mutant of plasmid pSC101. Mol. Microbiol. 5 (1991) 631-640