Potential artifacts in using a glutathione S-transferase fusion protein system and spin labeling electron paramagnetic resonance methods to study protein-protein interactions

Analytical Biochemistry

Volumn 376, Issue 1, 2008, Pages 160-162

  Antoniou, Chloe ^a   Fung, L W M ^a  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; ELECTRON RESONANCE; PARAMAGNETIC RESONANCE; PARAMAGNETISM; PEPTIDES;

BINDING PARTNERS; CYSTEINE RESIDUES; DATA INTERPRETATION; GLUTATHIONE S-TRANSFERASES; PROTEIN-PROTEIN INTERACTIONS; SITE-DIRECTED SPIN LABELING; SPECTRAL SUBTRACTIONS; SPIN LABELING;

ELECTRON SPIN RESONANCE SPECTROSCOPY;

GLUTATHIONE; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN;

ARTICLE; ARTIFACT; ELECTRON SPIN RESONANCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PROTEIN INTERACTION; SPIN LABELING;

EID: 41449114383     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.02.001     Document Type: Article

References (9)

1. Reichmann D., Rahat O., Cohen M., Neuvirth H., and Schreiber G. The molecular architecture of protein-protein binding sites. Curr. Opin. Struct. Biol. 17 (2007) 67-76
2. Fanucci G.E., and Cafiso D.S. Recent advances and applications of site-directed spin labeling. Curr. Opin. Struct. Biol. 16 (2006) 644-653
3. Chen M., Margittai M., Chen J., and Langen R. Investigation of alpha-synuclein fibril structure by site directed spin labeling. J. Biol. Chem. 282 (2007) 24970-24979
4. Mchaourab H.S., Lietzow M.A., Hideg K., and Hubbel W.L. Motion of spin-labeled side chains in T4 lysozyme. Correlation with protein structure and dynamics. Biochemistry 24 (1996) 7692-7704
5. Oldham W.M., Van Eps N., Preininger A.M., Hubbell W.L., and Hamm H.E. Mapping allosteric connections from the receptor to the nucleotide-binding pocket of heterotrimeric G proteins. Proc. Natl. Acad. Sci. USA 104 (2007) 7927-7932
6. Zhou A., DeSensi S.C., Stein R.A., Brandon S., Song L., Cobb C.E., Hustedt E.J., and Beth A.H. Structure of the cytoplasmic domain of erythrocyte band 3 hereditary spherocytosis variant P327R: Band 3 Tuscaloosa. Biochemistry 46 (2007) 10248-10257
7. Mehboob S., Jacob J., May M., Kotula L., Thiyagarajan P., Johnson M.E., and Fung L.W.-M. Structural analysis of the alpha N-terminal region of erythroid and nonerythroid spectrins by small-angle X-ray scattering. Biochemistry 44 (2003) 14702-14710
8. Berliner L.J., Grunwald J., Hankovsky O., and Hideg K. A novel reversible thiol-specific spin label: Papain active site labeling and inhibition. Anal. Biochem. 119 (1982) 450-455
9. Kiley P.J., and Storz G. Exploiting thiol modifications. PLOS Biol. 2 (2004) 1714-1717