Effect of hydroxyurea on procyclic Trypanosoma brucei: An unconventional mechanism for achieving synchronous growth

Eukaryotic Cell

Volumn 7, Issue 2, 2008, Pages 425-428

  Chowdhury, Arnab Roy ^a   Zhao, Zhixing ^a   Englund, Paul T ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

TRYPANOSOMA BRUCEI;

HYDROXYUREA; KINETOPLAST DNA;

ANIMAL; ARTICLE; CELL CYCLE G1 PHASE; CELL CYCLE S PHASE; DNA REPLICATION; DRUG EFFECT; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; TRYPANOSOMA BRUCEI;

ANIMALS; DNA REPLICATION; DNA, KINETOPLAST; G1 PHASE; HYDROXYUREA; S PHASE; TRYPANOSOMA BRUCEI BRUCEI;

EID: 41449087417     PISSN: 15359778     EISSN: None     Source Type: Journal    
DOI: 10.1128/EC.00369-07     Document Type: Article

References (20)

1. 3H]thymidine uptake of Trypanosoma brucei procyclic culture forms. J. Protozool. 27:122-128.
2. Cosgrove, W. B., M. J. Skeen, and S. L. Hajduk. 1979. Effects of hydroxyurea on Crithidia fasciculata. J. Protozool. 26:643-648.
3. Davis, P. K., A. Ho, and S. F. Dowdy. 2001. Biological methods for cell-cycle synchronization of mammalian cells. BioTechniques 30:1322-1331.
4. Galanti, N., J. A. Dvorak, J. Grenet, and J. P. McDaniel. 1994. Hydroxyureainduced synchrony of DNA replication in the Kinetoplastida. Exp. Cell Res. 214:225-230.
5. Hofer, A., P. P. Schmidt, A. Graslund, and L. Thelander. 1997. Cloning and characterization of the R1 and R2 subunits of ribonucleotide reductase from Trypanosoma brucei. Proc. Natl. Acad. Sci. USA 94:6959-6964.
6. Klingbeil, M. M., S. A. Motyka, and P. T. Englund. 2002. Multiple mitochondrial DNA polymerases in Trypanosoma brucei. Mol. Cell 10:175-186.
7. Kulikowicz, T., and T. A. Shapiro. 2006. Distinct genes encode type II topoisomerases for the nucleus and mitochondrion in the protozoan parasite Trypanosoma brucei. J. Biol. Chem. 281:3048-3056.
8. Liermann, B., G. Lassmann, and P. Langen. 1990. Quenching of tyrosine radicals of M2 subunit from ribonucleotide reductase in tumor cells by different antitumor agents: an EPR study. Free Radic. Biol. Med. 9:1-4.
9. Liu, B., Y. Liu, S. A. Motyka, E. E. Agbo, and P. T. Englund. 2005. Fellowship of the rings: the replication of kinetoplast DNA. Trends Parasitol. 21:363-369.
10. Liu, Y., and P. T. Englund. 2007. The rotational dynamics of kinetoplast DNA replication. Mol. Microbiol. 64:676-690.
11. Liu, Y., S. A. Motyka, and P. T. Englund. 2005. Effects of RNA interference of Trypanosoma brucei structure-specific endonuclease-I on kinetoplast DNA replication. J. Biol. Chem. 280:35513-35520.
12. Mutomba, M. C., and C. C. Wang. 1996. Effects of aphidicolin and hydroxyurea on the cell cycle and differentiation of Trypanosoma brucei bloodstream forms. Mol. Biochem. Parasitol. 80:89-102.
13. Pasion, S. G., G. W. Brown, L. M. Brown, and D. S. Ray. 1994. Periodic expression of nuclear and mitochondrial DNA replication genes during the trypanosomatid cell cycle. J. Cell Sci. 107:3515-3520.
14. Reichard, P., and A. Ehrenberg. 1983. Ribonucleotide reductase-a radical enzyme. Science 221:514-519.
15. Saxowsky, T. T., G. Choudhary, M. M. Klingbeil, and P. T. Englund. 2003. Trypanosoma brucei has two distinct mitochondrial DNA polymerase beta enzymes. J. Biol. Chem. 278:49095-49101.
16. Shlomai, J. 2004. The structure and replication of kinetoplast DNA. Curr. Mol. Med. 4:623-647.
17. Simpson, L., and P. Braly. 1970. Synchronization of Leishmania tarentolae by hydroxyurea. J. Protozool. 17:511-517.
18. Soto, M., L. Quijada, C. Alonso, and J. M. Requena. 2000. Histone synthesis in Leishmania infantum is tightly linked to DNA replication by a translational control. Biochem. J. 346:99-105.
19. Wang, Z., J. C. Morris, M. E. Drew, and P. T. Englund. 2000. Inhibition of Trypanosoma brucei gene expression by RNA interference using an integratable vector with opposing T7 promoters. J. Biol. Chem. 275:40174-40179.
20. Zick, A., I. Onn, R. Bezalel, H. Margalit, and J. Shlomai. 2005. Assigning functions to genes: identification of S-phase expressed genes in Leishmania major based on posttranscriptional control elements. Nucleic Acids Res. 33:4235-4242.