Extracellular nuclease from a thermophile, Streptomyces thermonitrificans: Purification and characterization of the deoxyribonuclease activity

FEMS Microbiology Letters

Volumn 237, Issue 2, 2004, Pages 273-278

  Deshmukh, Sumedha S ^a   Shankar, Vepatu ^a  

^a NATIONAL CHEMICAL LABORATORY   (India)

Author keywords

Characteristics; Endonuclease; Extracellular nuclease; Nuclease Stn ; Streptomyces thermonitrificans

Indexed keywords

3' MONONUCLEOTIDE; 5' MONONUCLEOTIDE; DEOXYRIBONUCLEASE; DIVALENT CATION; DOUBLE STRANDED DNA; MANGANESE; NUCLEASE; NUCLEOTIDE DERIVATIVE; OLIGONUCLEOTIDE; PHOSPHATE; PYROPHOSPHATE; RNA; SINGLE STRANDED DNA; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; HYDROLYSIS; NONHUMAN; PH; PRIORITY JOURNAL; STREPTOMYCES; STREPTOMYCES THERMONITRIFICANS; TEMPERATURE SENSITIVITY;

STREPTOMYCES; STREPTOMYCES THERMOVULGARIS;

EID: 4143149557     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.femsle.2004.06.056     Document Type: Article

References (19)

1. E.S. Rangarajan, and V. Shankar Sugar non-specific endonucleases FEMS Microbiol. Rev. 25 2001 583 613
2. W. Peczynska-Czoch, and M. Mordarski Actinomycetes enzymes M. Goodfellow S.T. Willams M. Mordarski Actinomycetes in Biotechnology 1988 Academic Press San Diego 219 283
3. S. Rangarajan, and V. Shankar Extracellular nuclease from Rhizopus stolonifer: purification and characteristics of â€" single strand preferential â€" deoxyribonuclease activity Biochim. Biophys. Acta 1473 1999 293 304
4. P.J. Curtis, M.G. Burdon, and R.M.S. Smellie The purification from rat liver of a nuclease hydrolysing ribonucleic acid and deoxyribonucleic acid Biochem. J. 98 1966 813 881
5. S. Gite, G. Reddy, and V. Shankar Active site characterization of S1 nuclease: affinity purification and influence of amino group modification Biochem. J. 285 1992 489 494
6. J.J. Sedmak, and S.E. Grossberg A rapid, sensitive, and versatile assay for protein using Coomassie Brilliant Blue G250 Anal. Biochem. 79 1977 544 552
7. S.S. Deshmukh, and V. Shankar Extracellular deoxyribonuclease production by a thermophile, Streptomyces thermonitrificans J. Ind. Microbiol. Biotechnol. 23 1999 709 712
8. 4 Nature (London) 227 1970 680 685
9. H. Blum, H. Beier, and H.J. Gross Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels Electrophoresis 8 1987 93 99
10. M. Dubois, K.A. Gilles, J.K. Hamilton, P.A. Rebers, and F. Smith Colorimetric method for determination of sugars and related substances Anal. Chem. 28 1956 350 356
11. E. Melgar, and D.A. Goldthwait Deoxyribonucleic acid nucleases: The effects of metals on the mechanism of action of deoxyribonuclease I J. Biol. Chem. 243 1968 4409 4416
12. G. Bernardi, and C. Sadron Studies on acid deoxyribonuclease. I. Kinetics of the initial degradation of acid deoxyribonucleic acid by acid deoxyribonuclease Biochemistry 3 1964 1411 1418
13. J.F. Aparicio, C. Hardisson, and J. Sanchez Purification and characterization of a nutritionally controlled endodeoxyribonuclease from Streptomyces glaucescens Biochem. J. 281 1992 231 237
14. S. Cal, J.F. Aparicio, C.G. De Los Reyes-Gavilan, R.G. Nicieza, and J. Sanchez A novel exocytoplasmic endonuclease from Streptomyces antibioticus Biochem. J. 306 1995 93 100
15. H.B. Gray Jr., T.P. Winston, J.L. Hodnett, R.J. Legerski, D.W. Nees, C.-F. Wei, and D.L. Robberson The extracellular nuclease from Alteromonas espejiana: An enzyme highly specific for non-duplex structure in nominally duplex DNAs J.G. Chirikijian T.S. Papas Gene Amplification and Analysis vol. 2 1981 Elsevier/North-Holland New York 169 203
16. S. Linn, and I.R. Lehman An endonuclease from mitochondria of Neurospora crassa J. Biol. Chem. 241 1966 2694 2699
17. W.K. Holloman, T.C. Rowe, and J.R. Rusche Studies on nuclease α from Ustilago maydis J. Biol. Chem. 256 1981 5087 5094
18. G. Bernardi, and C. Cordonnier Mechanism of degradation of DNA by endonuclease I from Escherichia coli J. Mol. Biol. 11 1965 141 143
19. V.N. Schumaker, E.G. Richards, and H.K. Schachman A study of the kinetic of the enzymatic digestion of deoxyribonucleic acid J. Am. Chem. Soc. 78 1956 4230 4236