A single, multiplex analysis for all relevant activating NRAS gene mutations using heteroduplex generators

British Journal of Haematology

Volumn 126, Issue 4, 2004, Pages 602-605

  Belli, Carolina ^a   Bowen, Derrick J ^b   De Brasi, Carlos ^a   Larripa, Irene ^a  

^a INST DE INVEST HEMATOLOGICAS   (Argentina)

^b CARDIFF UNIVERSITY   (United Kingdom)

Author keywords

Multiplex assay; NRAS oncogene; Universal Heteroduplex Generator

Indexed keywords

HETERODUPLEX;

ARTICLE; CODON; ELECTROPHORESIS; EXON; GENE ACTIVATION; GENE MUTATION; GENERATOR; HUMAN; NUCLEOTIDE SEQUENCE; ONCOGENE N RAS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SCREENING; UNIVERSAL HETERODUPLEX GENERATOR; WORKLOAD;

BASE SEQUENCE; GENES, RAS; HETERODUPLEX ANALYSIS; HUMANS; MOLECULAR SEQUENCE DATA; MUTATION; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 4143084823     PISSN: 00071048     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2141.2004.05079.x     Document Type: Article

References (12)

1. Adjei, A. (2001) Blocking oncogenic ras signalling for cancer therapy. Journal National Cancer Institute, 93, 1062-1073.
2. Bar-Eli, M., Ahuja, H., Gonzalez-Cadavid, N., Foti, A. & Cline, M. (1989) Analysis of N-ras exon-1 mutations in myelodysplastic syndromes by polymerase chain reaction and direct sequencing. Blood, 73, 281-283.
3. Belli, C., DeBrasi, C. & Larripa, I. (2003) Rapid detection of exon 1 NRAS gene mutations using Universal Heteroduplex Generator technology. Human Mutation, 21, 132-137.
4. Bowen, D., Standen, G., Granville, S., Bowley, S., Wood, N. & Bidwell, J. (1997) Genetic diagnosis of factor V Leiden using heteroduplex technology. Thrombosis and Haemostasis, 77, 119-122.
5. Brown, R., Marshall, C., Pennie, S. & Hall, A. (1984) Mechanism of activation of an N-ras gene in the human fibrosarcoma cell line HT1080. EMBO Journal, 3, 1321-1326.
6. Burvall, K., Ridanpää, M., Husgafvel-Pursiainen, K. & Önfelt, A. (1993) Separation of transforming N-ras mutations in codons 12, 13 and 61 by denaturing gradient gel electrophoresis: investigation based on a set of phagemid construct. Mutation Research, 285, 287-294.
7. Farr, C., Saiki, R., Erlich, H., Mc Cormick, F. & Marshall, C. (1988) Analysis of RAS gene mutations in acute myeloid leukemia by polymerase chain reaction and oligonucleotide probes. Proceedings of the National Academy of Sciences of the United States of America, 85, 1629-1633.
8. Lubbert, M., Mertelsmann, R. & Herrmann, F. (1992) Detection of allele-specific expression of N-RAS oncogenes in human leukaemia cells. British Journal of Haematology, 81, 370-373.
9. Neri, A., Knowles, D., Greco, A., McCormick, F. & Dalla-Favera, R. (1988) Analysis of RAS oncogene mutations in human limphoid malignancies. Proceedings of the National Academy of Sciences of the United States of America, 85, 9268-9272.
10. Sheng, X., Kawamura, M., Ohnishi, H., Ida, K., Hanada, R., Kojima, S., Kobayashi, M., Bessho, F., Yanagisawa, M. & Hayashi, Y. (1997) Mutations of ras genes in childhood acute myeloid leukemia, myelodysplastic syndrome and juvenile chronic myelocytic leukemia. Leukemia Research, 21, 697-701.
11. Velaan de Vries, M., Bogaard, M.E., Van den Elst, H., Van Boom, J., Van der Eb, A. & Bos, J. (1986) A dot-blot screening procedure for mutated ras oncogenes using synthetic oligonucleotides. Gene, 50, 313-320.
12. Wood, N., Standem, G., Murray, E.W., Lillicrap, D., Holmberg, L., Peake, I.R. & Bidwell, J. (1995) Rapid genotype analysis in type 2B von Willebrand's disease using a universal heteroduplex generator. British Journal of Haematology, 89, 152-156.