Cloning and expression of hepatitis C virus core protein in pGemex-1 expression vector

Archives of Iranian Medicine

Volumn 11, Issue 2, 2008, Pages 173-178

  Kazemi, Bahram ^a   Bandehpour, Mojgan ^a,b   Seyed, Negar ^a,c   Roozbehi, Mehrnaz ^a   Mosaffa, Nariman ^a  

^a SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^b NATIONAL INSTITUTE OF GENETIC ENGINEERING AND BIOTECHNOLOGY   (Iran)

^c PASTEUR INSTITUTE OF IRAN   (Iran)

Author keywords

Gene expression; Hepatitis C virus

Indexed keywords

COMPLEMENTARY DNA; HEPATITIS C ANTIBODY; HEPATITIS C VACCINE; HEPATITIS C VIRUS CORE PROTEIN; ISOPROPYL THIOGALACTOSIDE; UNCLASSIFIED DRUG; VIRUS RNA;

ARTICLE; BACTERIOPHAGE T7; BLOOD SAMPLING; CELL LYSATE; CONTROLLED STUDY; DOT HYBRIDIZATION; DRUG SYNTHESIS; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE AMPLIFICATION; HEPATITIS C; HUMAN; MOLECULAR CLONING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROMOTER REGION; PROTEIN EXPRESSION; RECOMBINANT PLASMID;

CLONING, MOLECULAR; DNA, COMPLEMENTARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION; GENETIC VECTORS; HUMANS; PLASMIDS; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; VIRAL CORE PROTEINS;

EID: 41149177495     PISSN: 10292977     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. WHO. Global surveillance and control of hepatitis C. Report of a WHO Consultation organized in collaboration with the Viral Hepatitis Prevention Board, Antwerp, Belgium. J Viral Hepat. 1999; 6: 35-47.
2. Arichi T, Saito T, Major ME, Belyakov IM, Shirai M, Engelhard VH, et al. Prophylactic DNA vaccine for hepatitis C virus (HCV) infection: HCV-specific cytotoxic T lymphocyte induction and protection from HCV-recombinant vaccinia infection in an HLA-A2.1 transgenic mouse model. Proc Natl Acad Sci USA. 2000; 97: 297-302.
3. Majeau N, Gagne V, Boivin A, Bolduc M, Majeau JA, Ouellet D, et al. The N-terminal half of the core protein of hepatitis C virus is sufficient for nucleocapsid formation. J Gen Virol. 2004; 85: 971-981.
4. van Pelt JF, Severi T, Crabbe T, Eetveldt AV, Verslype C, Roskams T, et al. Expression of hepatitis C virus core protein impairs DNA repair in human hepatoma cells. Cancer Lett. 2004; 209: 197-205.
5. Cristofari G, Ivanyi-Nagy R, Gabus C, Boulant S, Lavergne JP, Penin F, et al. The hepatitis C virus core protein is a potent nucleic acid chaperone that directs dimerization of the vital (+) strand RNA in vitro. Nucleic Acids Res. 2004; 32: 2623-2631.
6. Siavoshian S, Abraham JD, Kieny MP, Schuster C. HCV core, NS3, NS5A, and NS5B proteins modulate cell proliferation independently from p53 expression in hepatocarcinoma cell lines. Arch Virol. 2004; 149: 323-336.
7. Shimoike T, Mimori S, Tani H, Matsuura Y, Miyamura T. Interaction of hepatitis C virus core protein with viral sense RNA and suppression of its translation. J Virol. 1999; 73: 9718-9725.
8. Kazemi B, Tafvizi F, Bandehpour M. Determination of HCV genotypes in Iran. Biotechnology. 2005; 4: 139-143.
9. Pfeffer U. One-tube RT-PCR with sequence-specific primers. In: Rapley R, Manning DL, eds. RNA Isolation and Characterization Protocols. Totowa, N.J.: Humana Press; 1998: 143-151.
10. Pherson MC, Moller MJ. PCR: The Basics from Background to Bench. Oxford, UK: BIOS Scientific publishers; 2000: 9-21.
11. Eun HM. Enzymology primer for Recombinant DNA Technology. San Diego: Academic Press. 1996: 345-489.
12. Gaastra W, Klemm P. Radio labeling of DNA with 3 terminal transferase. In: Walker JM, ed. Methods in Molecular Biology. Vol 2. Clifton: Humana Press; 1984: 269-271.
13. Gaastra W, Hansen K. Ligation of DNA with T4 DNA ligase. In: Walker JM, ed. Methods in Molecular Biology. Vol 2. Clifton: Humana Press; 1984: 225-230.
14. Hanahan D. Studies on transformation on E. coli with plasmids. J Mol Biol. 1983; 98: 503-517.
15. Feliciello I, Chinali G. A modified alkaline lyses method for the preparation of highly purified plasmid DNA from Escherichia coli. Anal Biochem. 1993; 212: 394-401.
16. Spiro MJ, Bhoyroo VD, Spiro RG. Molecular cloning and expression of rat liver endo-alfa mannosidase, an N-linked oligosaccharide processing enzyme. J Biolo Chemistry. 1997; 272: 29356-29363.
17. Smith BJ. SDS polyacrylamide gel electrophoresis of proteins. In: Walker JM, ed. Method in Molecular Biology. Vol 1. Clifton: Humana Press; 1984: 41-56.
18. Smith BJ. Acetic Acid[ - Urea Polyacrylamide Gel Electrophoresis of Proteins. In: Walker John, ed. Methods in Molecular Biology. Vol 1. Clifton: Humana Press; 1984: 63-73.
19. Hudsun L, Hay FC. Antibody interaction with antigen. In: Hudsun L, Hay FC. Practical Immunology. 3rd ed. Oxford; Boston: Blackwell Scientific Publication; 1998; 207-263.
20. Shewry PR, Fido RJ. Protein blotting, Principles and Applications. In: Rapley R, Walker JM, eds. Molecular Biomethods Handbook. Totowa, NJ: Humana Press. 1998: 435-444.
21. Encke J, Findeklee J, Geib J, Pfaff E, Stremmel W. Prophylactic and therapeutic vaccination with dendritic cells against hepatitis C virus infection. Clin Exp Immunol. 2005; 142: 362-369
22. Gehring S, Gregory SH, Kuzushita N, Wands JR. Type 1 interferon augments DNA-based vaccination against hepatitis C virus core protein. J Med Virol. 2005; 75: 249-257.
23. Aguilar JC, Acosta-Rivero N, Duenas-Carrera S, Morales-Grillo J, Pichardo D, Urquiza D, et al. HCV core protein modulates the immune response against the HBV surface antigen in mice. Biochem Biophys Res Commun. 2003; 310: 59-63.
24. Matsui M, Moriya O, Akatsuka T. Enhanced induction of hepatitis C virus-specific cytotoxic T lymphocytes and protective efficacy in mice by DNA vaccination followed by adenovirus boosting in combination with the interleukin-12 expression plasmid. Vaccine. 2003; 21: 1629-1639.
25. Duenas-Carrera S, Alvarez-Lajonchere L, Alvarez-Obregon JC, Herrera A, Lorenzo LJ, Pichardo D, et al. A truncated variant of the hepatitis C virus core induces a slow but potent immune response in mice following DNA immunization. Vaccine. 2000; 19: 992-997.