Role of histidine residues in EcoP15I DNA methyltransferase activity as probed by chemical modification and site-directed mutagenesis

Biochemical Journal

Volumn 410, Issue 3, 2008, Pages 543-553

  Jois, Prashanth S ^a   Madhu, Nagaraj ^a   Rao, Desirazu N ^a  

^a INDIAN INSTITUTE OF SCIENCE   (India)

Author keywords

Base flipping; Chemical modification; DNA methyltransferase (MTase); Histidine modification; S adenosyl L methionine (AdoMet); Site directed mutagenesis

Indexed keywords

BASE FLIPPING; DNA METHYLTRANSFERASE (MTASE); HISTIDINE MODIFICATION; S-ADENOSYL-L-METHIONINE (ADOMET); SITE-DIRECTED MUTAGENESIS;

BINDING SITES; CHEMICAL MODIFICATION; CHROMATOGRAPHY; ENZYME ACTIVITY; MUTAGENESIS; PROTEINS;

DNA;

ALANINE; DIETHYL PYROCARBONATE; DNA METHYLTRANSFERASE; ECOP151 DNA METHYLTRANSFERASE; HISTIDINE; HISTIDINE DERIVATIVE; HYDROXYLAMINE; N CARBOXYHISTIDINE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; CHEMICAL MODIFICATION; CHRONOTHERAPY; CONTROLLED STUDY; DNA BINDING; DNA METHYLATION; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME INACTIVATION; ENZYME STRUCTURE; GEL FILTRATION CHROMATOGRAPHY; INCUBATION TIME; MUTANT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; SPECTRUM; SURFACE PLASMON RESONANCE;

BASE SEQUENCE; CATALYSIS; CIRCULAR DICHROISM; DNA METHYLATION; DNA MODIFICATION METHYLASES; DNA PRIMERS; ELECTROPHORETIC MOBILITY SHIFT ASSAY; ESCHERICHIA COLI; GENETIC VECTORS; HISTIDINE; MOLECULAR PROBES; MUTAGENESIS, SITE-DIRECTED; PLASMIDS; SPECTROMETRY, FLUORESCENCE; SURFACE PLASMON RESONANCE;

EID: 41149169628     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20070900     Document Type: Article

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