Serological diagnosis of syphilis: Enzyme-linked immunosorbent assay to measure antibodies to individual recombinant Treponema pallidum antigens

Journal of Immunoassay and Immunochemistry

Volumn 29, Issue 2, 2008, Pages 143-151

  Martin, Irene E ^a   Lau, Allan ^a   Sawatzky, Pam ^a   Tsang, Raymond S W ^a   Cuff, Wilfred ^a   Lee, Craig ^b   MacPherson, Paul A ^b   Mazzulli, Tony ^c  

^a PUBLIC HEALTH AGENCY OF CANADA   (Canada)

^b UNIVERSITY OF OTTAWA   (Canada)

^c MOUNT SINAI HOSPITAL   (Canada)

Author keywords

EIA; Recombinant antigens; Syphilis

Indexed keywords

RECOMBINANT ANTIGEN; TREPONEMA PALLIDUM RECOMBINANT ANTIGEN; UNCLASSIFIED DRUG;

ANTIBODY BLOOD LEVEL; ARTICLE; ENZYME IMMUNOASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; NONHUMAN; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SYPHILIS; SYPHILIS SEROLOGY; TREPONEMA PALLIDUM;

ANTIBODIES, BACTERIAL; ANTIGENS, BACTERIAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; RECOMBINANT PROTEINS; SENSITIVITY AND SPECIFICITY; SYPHILIS; TREPONEMA PALLIDUM;

EID: 41149167654     PISSN: 15321819     EISSN: 15324230     Source Type: Journal    
DOI: 10.1080/15321810801887771     Document Type: Article

References (18)

1. Egglestone, S.L.; Turner, A.J.L. Serological diagnosis of syphilis. Commun. Dis. Public Hlth. 2000, 3 (3), 158-162.
2. Larsen, S.A.; Steiner, B.M.; Rudolph, A.H. Laboratory diagnosis and interpretation of tests for syphilis. Clin. Microbiol. Rev. 1995, 8 (1), 1-21.
3. Peter, C.R.; Thompson, M.A.; Wilson, D.L. False-positive reactions in the rapid plasma reagin-card, fluorescent treponemal antibody-absorbed, and hemagglutination treponemal syphilis serology tests. J. Clin. Microbiol. 1979, 9 (3), 369-372.
4. Calonge, N. U.S. Preventive Services Task Force. Screening for syphilis infection: recommendation statement. Ann. Fam. Med. 2004, 2 (4), 362-365.
5. Fraser, C.M.; Norris, S.J.; Weinstock, G.M.; White, O.; Sutton, G.G.; Dodson, R.; Gwinn, M.; Hickey, B.K.; Clayton, R.; Ketchum, K.A.; Sodergren, E.; Hardham, J.M.; McLeod, M.P.; Salzberg, S.; Peterson, J.; Khalak, H.; Richardson, D.; Howell, J.K.; Chidambaram, M.; Utterback, T.; McDonald, L.; Artiach, P.; Bowman, C.; Cotton, M.D.; Fujii, C.; Garland, S.; Hatch, B.; Horst, K.; Roberts, K.; Sandusky, M.; Weidman, J.; Smith, H.O.; Venter, J.C. Complete genome sequence of Treponema pallidum, the syphilis spirochete. Science 1998, 281 (5375), 375-388.
6. Zrein, M.; Maure, I.; Boursier, F.; Soufflet, L. Recombinant antigen-based enzyme immunoassay for screening of Treponema pallidum antibodies in blood bank routine. J. Clin. Microbiol. 1995, 33 (3), 525-527.
7. Young, H.; Moyes, A.; Seagar, L.; McMillan, A. Novel recombinant-antigen enzyme immunoassay for serological diagnosis of syphilis. J. Clin. Microbiol. 1998, 36 (4), 913-917.
8. Cole, M.J.; Perry, K.R.; Parry, J.V. Comparative evaluation of 15 serological assays for the detection of syphilis infection. Eur. J. Clin. Microbiol. Infect. Dis. 2007, 26 (10), 705-713.
9. Meyer, M.F.; Eddy, T.; Baughn, R.E. Analysis of Western blotting (immunoblotting) technique in diagnosis of congenital syphilis. J. Clin. Microbiol. 1994, 32 (3), 629-633.
10. de Lemos, E.A.; Belem, Z.R.; Santos, A.; Ferreira, A.W. Characterization of the Western blotting IgG reactivity patterns in the clinical phases of acquired syphilis. Diagn. Microbiol. Infect. Dis. 2007, 58 (2), 177-183.
11. Tsang, R.S.W.; Martin, I.E.; Lau., A.; Sawatzky, P. Serological diagnosis of syphilis: comparison of the Trep-Chek IgG enzyme immunoassay with other screening and confirmatory tests. FEMS. Immunol. Med. Microbiol. 2007, 51 (1), 118-124.
12. Levesque, R. SPSS Programming and Data Management: A Guide for SPSS and SAS Users, 4th edn.; SPSS Inc.: Chicago, Illinois, 2007, (http://www.spss.com/spss/SPSSdatamgmt_4e.pdf).
13. Ijsselmuiden, O.E.; Schouls, L.M.; Stolz, E.; Aelbers, G.N.; Agterberg, C.M.; Top, J.; van Embden, J.D.A. Sensitivity and specificity of an enzyme-linked immunosorbent assay using the recombinant DNA-derived Treponema pallidum protein TmpA for serodiganosis of syphilis and the potential use of TmpA for assessing the effect of antibiotic therapy. J. Clin. Microbiol. 1989, 27 (1), 152-157.
14. Norris, S.J. Polypeptides of Treponema pallidum: progress towards understanding their structural, functional, and immunologic roles. Treponema Pallidum Polypeptide Research Group. Microbiol. Rev. 1993, 57 (3), 750-779.
15. Byrne, R.E.; Laska, S.; Bell, M.; Larson, D.; Phillips, J.; Todd, J. Evaluation of a Treponema pallidum western immunoblot assay as a confirmatory test for syphilis. J. Clin. Microbiol. 1992, 30 (1), 115-122.
16. Backhouse, J.L.; Nesteroff, S.I. Treponema pallidum western blot: comparison with the FTA-ABS test as a confirmatory test for syphilis. Diagn. Microbiol. Infect. Dis. 2001, 39 (1), 9-14.
17. Interpretation and use of the Western blot assay for serodiagnosis of human immunodeficiency virus type 1 infections. M.M.W.R. 1989, 38 (Suppl.7), 1-7 Centers for Disease Control.
18. Yeh, C.T.; Han, C.M.; Lo, S.Y.; Ou, J.H.; Fan, K.D.; Sheen, I.S.; Chu, C.M.; Liaw, Y.F. Early detection of anti-HCc antibody in acute hepatitis C virus (HCV) by Western blot (immunoblot) using a recombinant HCV core protein fragment. J. Clin. Microbiol. 1994, 32 (9), 2235-2241.