Generation and utilization of P450 cholesterol side-chain cleavage enzyme and 3β-hydroxysteroid dehydrogenase antibodies for universal detection

Journal of Immunoassay and Immunochemistry

Volumn 29, Issue 2, 2008, Pages 152-160

  Chiu, Chih Hsien ^a   Wei, Hen Wei ^a   Wu, Leang Shin ^a  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

Author keywords

3 HSD; Bovine corpus luteum; P450scc; Polyclonal antibody; Steroidogenesis

Indexed keywords

3(OR 17)BETA HYDROXYSTEROID DEHYDROGENASE; CHOLESTEROL MONOOXYGENASE (SIDE CHAIN CLEAVING); CYTOCHROME P450; POLYCLONAL ANTIBODY;

ANIMAL TISSUE; ARTICLE; CORPUS LUTEUM; IMMUNOHISTOCHEMISTRY; LUTEAL CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; STEROIDOGENESIS; WESTERN BLOTTING;

3-HYDROXYSTEROID DEHYDROGENASES; ANIMALS; ANTIBODIES; BLOTTING, WESTERN; CATTLE; COMPLEX MIXTURES; CORPUS LUTEUM; CYTOCHROME P-450 ENZYME SYSTEM; FEMALE; GONADAL STEROID HORMONES; RABBITS; RECOMBINANT PROTEINS;

EID: 41149131737     PISSN: 15321819     EISSN: 15324230     Source Type: Journal    
DOI: 10.1080/15321810801887839     Document Type: Article

References (15)

1. Rodway, M.R.; Swan, C.L.; Crellin, N.K.; Gillio-Meina, C.; Chedrese, P.J. Steroid regulation of progesterone synthesis in a stable porcine granulosa cell line: a role for progestins. J. Steroid. Biochem. Mol. Biol. 1999, 68, 173-180.
2. Chaffin, C.L.; Dissen, G.A.; Stouffer, R.L. Hormonal regulation of steroidogenic enzyme expression in granulosa cells during the peri-ovulatory interval in monkeys. Mol. Hum. Reprod. 2000, 6, 11-18.
3. Bao, B.; Garverick, H.A. Expression of steroidogenic enzyme and gonadotropin receptor genes in bovine follicles during ovarian follicular waves: a review. J. Animal Sci. 1998, 76, 1903-1921.
4. Ford, S.L.; Reinhart, A.J.; Lukyanenko, Y.; Hutson, J.C.; Stocco, D.M. Pregnenolone synthesis in immature rat Sertoli cells. Mol. Cell. Endocrinol. 1999, 157, 87-94.
5. Sasano, H.; Sasano, N.; Okamoto, M. Immunohistochemical demonstration of cholesterol side-chain cleavage cytochrome P-450 in bovine and human adrenals. Pathol. Res. Pract. 1989, 184, 337-342.
6. Lamberski, J.A.; Thompson, N.E.; Burgess, R.R. Expression and purification of a single-chain variable fragment antibody derived from a polyol-responsive monoclonal antibody. Protein Expr. Purif. 2006, 47, 82-92.
7. Braunstein, J.D.; Halwer, M. Bradford protein assay and determination of estrogen and progesterone receptors. Clin. Chem. 1986, 32, 1588-1589.
8. Verma, N.K.; Sodhi, R.; Rajput, Y.S. Anti-idiotypic antibodies against bovine growth hormone. Asian-Aust. J. Animal Sci. 2003, 16 (5), 732-737.
9. Kohen, P.; Castro, O.; Palomino, A.; Munoz, A.; Christenson, L.K.; Sierralta, W.; Carvallo, P.; Strauss, J.F., III; Devoto, L. The steroidogenic response and corpus luteum expression of the steroidogenic acute regulatory protein after human chorionic gonadotropin administration at different times in the human luteal phase. J. Clin. Endocrinol. Metab. 2003, 88, 3421-3430.
10. Isobe, N.; Nakao, T.; Yoshimura, Y. Distribution of cytochrome P450-side chain cleavage in the theca interna layers of bovine small antral and cystic follicles. Reprod. Domest. Anim. 2003, 38, 405-409.
11. Lai, W.W.; Hsiao, P.H.; Guiguen, Y.; Chung, B.C. Cloning of zebrafish cDNA for 3beta-hydroxysteroid dehydrogenase and P450scc. Endocr. Res. 1998, 24, 927-931.
12. Conley, A.J.; Kaminski, M.A.; Dubowsky, S.A.; Jablonka-Shariff, A.; Redmer, D.A.; Reynolds, L.P. Immunohistochemical localization of 3 β-hydroxysteroid dehydrogenase and P450 17 α-hydroxylase during follicular and luteal development in pigs, sheep, and cows. Biol. Reprod. 1995, 52, 1081-1094.
13. Kobayashi, M.; Nakano, R.; Ooshima, A. Immunohistochemical localization of pituitary gonadotrophins and gonadal steroids confirms the 'two-cell, two-gonadotrophin' hypothesis of steroidogenesis in the human ovary. J. Endocrinol. 1990, 126, 483-488.
14. Pescador, N.; Soumano, K.; Stocco, D.M.; Price, C.A.; Murphy, B.D. Steroidogenic acute regulatory protein in bovine corpora lutea. Biol. Reprod. 1996, 55, 485-491.
15. Fairchild, D.L.; Pate, J.L. Modulation of bovine luteal cell synthetic capacity by interferon-gamma. Biol. Reprod. 1991, 44, 357-363.