Quantification of viable cells of Clavibacter michiganensis subsp. michiganensis using a DNA binding dye and a real-time PCR assay

Plant Pathology

Volumn 57, Issue 2, 2008, Pages 332-337

  Luo, L X ^a   Walters, C ^b   Bolkan, H ^b   Liu, X L ^a   Li, J Q ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

^b Campbell Vegetable R and D   (United States)

Author keywords

Bacterial canker of tomato; Ethidium monoazide; Lycopersicon esculentum; Quantitative RT PCR; TaqMan probe

Indexed keywords

BACTERIUM; DNA; DYE; FRUIT; PATHOGEN; POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS); CLAVIBACTER MICHIGANENSIS SUBSP. MICHIGANENSIS; LYCOPERSICON ESCULENTUM;

EID: 41049109194     PISSN: 00320862     EISSN: 13653059     Source Type: Journal    
DOI: 10.1111/j.1365-3059.2007.01736.x     Document Type: Article

References (22)

1. Bach HJ, Jessen I, Schloter M, Munch JC, 2003. A TaqMan-PCR protocol for quantification and differentiation of the phytopathogenic Clavibacter michiganensis subspecies. Journal of Microbiological Methods 52, 85 91.
2. Biggerstaff CM, Gleason ML, Braun EJ, 2000. Refinement of a nondestructive tomato seed assay for Clavibacter michiganensis subsp. michiganensis using seed fiber. Seed Science and Technology 28, 261 9.
3. Bolkan H, Waters CM, Fatmi M, 1996. ISTA Handbook on Seed Health Testing. Working sheet No. 67. Zurich, Switzerland : International Seed Testing Association.
4. Boltont PH, Kearns DR, 1978. Spectroscopic properties of ethidium monoazide: a fluorescent photoaffinity label for nucleic acids. Nucleic Acids Research 5, 4891 903.
5. Carlton WM, Gleason ML, Braun EJ, 1992. Entry of Clavibacter michiganensis subsp. michiganensis into tomato plants through pruning wounds. Phytopathology 82, 1153 (Abstr RJ, Ries SM, Pataky JK, 1991. Dissemination of Clavibacter michiganensis subsp. michiganensis by practices used to produce tomato plants. Phytopathology 81, 1276 81.
6. Dreier J, Bermpohl A, Eichenlaub R, 1995. Southern hybridization and PCR for specific detection of phytopathogenic Clavibacter michiganensis subsp. michiganensis. Phytopathology 85, 462 8.
7. European Union, 1995. Commission directive 95/4/EC amendment of 21 Feb 1995 to the European Community Plant Health Directive (77/93/EEC). Official Journal of the European Communities L44, 56 60.
8. Fatmi M, Schaad NW, 2002. Survival of Clavibacter michiganensis subsp. michiganensis in infected tomato stems under natural field conditions in California, Ohio and Morocco. Plant Pathology 51, 149 54.
9. Gleason ML, Braun EJ, Cariton WM, Peterson RH, 1991. Survival and dissemination of Clavibacter michiganensis subsp. michiganensis in tomato. Phytopathology 81, 1519 23.
10. Gleason ML, Gitaitis RD, Ricker MD, 1993. Recent progress in understanding and controlling bacterial canker of tomato in Eastern North America. Plant Disease 77, 1069 76.
11. Hixon C, White W, Yielding J, 1975. Selective covalent binding of an ethidium analog to mitochondrial DNA with production of petite mutants in yeast by photoaffinity labeling. Journal of Molecular Biology 92, 319 29.
12. Jahr H, Bahro R, Bruger A, Ahlemeyer J, Eichenlaub R, 1999. Interactions between Clavibacter michiganensis and its host plants. Environmental Microbiology 1, 113 8.
13. Li X, De Boer S, 1995a. Comparison of 16S ribosomal RNA genes in Clavibacter michiganensis subspecies with other coryneform bacteria. Canadian Journal of Microbiology 41, 925 9.
14. Li X, De Boer S, 1995b. Selection of polymerase chain reaction primers from an RNA intergenic spacer region for specific detection of Clavibacter michiganensis subsp. sepedonicus. Phytopathology 85, 837 42.
15. Louws F, Rademaker J, De Bruijn F, 1999. The three Ds of PCR-based genomic analysis of phytobacteria: diversity, detection, and disease diagnosis. Annual Review of Phytopathology 37, 81 125.
16. Nogva HK, Dromtorp SM, Nissen H, Rudi K, 2003. Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5′-nuclease PCR. BioTechniques 34, 812 3.
17. O'Brien M, Boton W, 1995. Comparison of cell viability probes compatible with fixation and permeabilization for combined surface and intracellular staining in flow cytometry. Cytometry 19, 243 55.
18. Riedy M, Muirhead K, Jensen C, Stewart C, 1991. Use of a photolabeling technique to identify nonviable cells in fixed homologous or heterologous cell populations. Cytometry 12, 133 9.
19. Schaad NW, Berthier-Schaad Y, Sechler A, Knorr D, 1999. Detection of Clavibacter michiganensis subsp. sepedonicus in potato tubers by Bio-PCR and automated real-time fluorescence detection system. Plant Disease, 83, 1095 100.
20. Schaad NW, Frederick RD, Shaw J et al., 2003. Advances in molecular-based diagnostics in meeting crop biosecurity and phytosanitary issues. Annual Review of Phytopathology 41, 305 24.
21. Uyttendaele M, Bastiaansen A, Debevere J, 1997. Evaluation of the NASBA nucleic acid amplification system for assessment of the viability of Campylobacter jejuni. International Journal of Food Microbiology 37, 13 20.
22. Wang S, Levin RE, 2006. Discrimination of viable Vibrio vulnificus cells from dead cells in real-time PCR. Journal of Microbiological Methods 64, 1 8.