Optimized Sample Preparation for MALDI Mass Spectrometry Analysis of Protected Synthetic Peptides

Journal of the American Society for Mass Spectrometry

Volumn 19, Issue 4, 2008, Pages 614-619

  Schaiberger, Audrey M ^a   Moss, Jason A ^a  

^a Roche Colorado   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADDITIVES; IONIZATION POTENTIAL; MASS SPECTROMETRY; SOLUBILITY; SYNTHESIS (CHEMICAL);

MATRIX ASSISTED LASER DESORPTION/IONIZATION (MALDI) MASS SPECTROMETRY; SOLID PHASE SYNTHESIS;

PEPTIDES;

CESIUM; CESIUM CHLORIDE; CRYSTALLIN; DITHRANOL; SYNTHETIC PEPTIDE;

ARTICLE; FRAGMENTATION REACTION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MONITORING; MORPHOLOGY; PROTEIN PROCESSING; QUALITY CONTROL; REACTION ANALYSIS; SAMPLE;

HIV ENVELOPE PROTEIN GP41; HIV FUSION INHIBITORS; PEPTIDE FRAGMENTS; SPECIMEN HANDLING; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 41049092039     PISSN: 10440305     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jasms.2008.01.010     Document Type: Article

References (22)

1. Bruckdorfer T., Marder O., and Albericio F. From Production of Peptides in Milligram Amounts for Research to Multi-Ton Quantities for Drugs of the Future. Curr. Pharm. Biotech. 5 (2004) 29-43
2. Bray B.L. Large-Scale Manufacture of Peptide Therapeutics by Chemical Synthesis. Nat. Rev. Drug Discov. 2 (2003) 587-593
3. Stults J.T. Matrix-Assisted Laser-Desorption Ionization Mass-Spectrometry (MALDI-MS). Curr. Opin. Struct. Biol. 5 (1995) 691-698
4. Skelton R., Dubois F., and Zenobi R. A MALDI Sample Preparation Method Suitable for Insoluble Polymers. Anal. Chem. 72 (2000) 1707-1710
5. Zhang H., Zhang C., Lajoie G., and Yeung K.K.-C. Selective Sampling of Phosphopeptides for Detection by MALDI Mass Spectrometry. Anal. Chem. 77 (2005) 6078-6084
6. Norris J.L., Porter N.A., and Caprioli R.M. Mass Spectrometry of Intracellular and Membrane Proteins Using Cleavable Detergents. Anal. Chem. 75 (2003) 6642-6647
7. Ekstrom S., Ericsson D., Onnerfjord P., Bengtsson M., Nilsson J., Marko-Varga G., and Laurell T. Signal Amplification Using "Spot on-a-Chip" Technology for the Identification of Proteins via MALDI-TOF MS. Anal. Chem. 73 (2001) 214-219
8. Distler A.M., and Allison J. Improved MALDI-MS Analysis of Oligonucleotides through the Use of Fucose as a Matrix Additive. Anal. Chem. 73 (2001) 5000-5003
9. Fitzgerald M.C., Parr G.R., and Smith L.M. Basic Matrices for the Matrix-Assisted Laser-Desorption Ionization Mass-Spectrometry of Proteins and Oligonucleotides. Anal. Chem. 65 (1993) 3204-3211
10. Beavis R.C., and Chait B.T. Cinnamic Acid Derivatives as Matrices for Ultraviolet Laser Desorption Mass Spectrometry of Proteins. Rapid Commun. Mass Spectrom. 3 (1989) 432-435
11. Beavis R.C., Chaudhary T., and Chait B.T. α-Cyano-4-Hydroxycinnamic Acid as a Matrix for Matrix-Assisted Laser Desorption Mass Spectrometry. Org. Mass Spectrom. 27 (1992) 156-158
12. Strupat K., Karas M., and Hillenkamp F. 2,5-Dihydroxybenzoic Acid: A New Matrix for Laser Desorption-Ionization Mass Spectrometry. Int. J. Mass Spectrom. Ion Processes. 111 (1991) 89-102
13. Nordhoff E., Kirpekar F., and Roepstorff P. Mass Spectrometry of Nucleic Acids. Mass Spectrom. Rev. 15 (1996) 67-138
14. Montaudo G., Montaudo M.S., Puglisi C., and Samperi F. 2-(4-Hydroxyphenylazo)-Benzoic Acid: A Solid Matrix for Matrix-assisted Laser Desorption/Ionization of Polystyrene. Rapid Commun. Mass Spectrom. 8 (1994) 1011-1015
15. Liu H.M.D., and Schlunegger U.P. Matrix-Assisted Laser Desorption/Ionization of Synthetic Polymers with Azo Compound Matrices. Rapid Commun. Mass Spectrom. 10 (1996) 483-489
16. Macha S.F., Limbach P.A., Hanton S.D., and Owens K.G. Silver Cluster Interferences in Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry of Nonpolar Polymers. J. Am. Soc. Mass Spectrom. 12 (2001) 732-743
17. Pastor S.J., and Wilkins C.L. Analysis of Hydrocarbon Polymers by Matrix-Assisted Laser Desorption/Ionization-Fourier Transform Mass Spectrometry. J. Am. Soc. Mass Spectrom. 8 (1997) 225-233
18. Belu A.M., DeSimone J.M., Linton R.W., Lange G.W., and Friedman R.M. Evaluation of Matrix-Assisted Laser Desorption Ionization Mass Spectrometry for Polymer Characterization. J. Am. Soc. Mass Spectrom. 7 (1996) 11-24
19. pKa values were calculated using Chemsketch version 5.0 (Advanced Chemistry Development, Toronto, Canada).
20. Smirnov I.P., Zhu X., Taylor T., Huang Y., Ross P., Papayanopoulos I.A., Martin S.A., and Pappin D.J. Suppression of α-Cyano-4-Hydroxycinnamic Acid Matrix Clusters and Reduction of Chemical Noise in MALDI-TOF Mass Spectrometry. Anal. Chem. 76 (2004) 2958-2965
21. Fukuzawa S., Asanuma M., Tachibana K., and Hirota H. On-Probe Sample Preparation without Washes for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Using an Anion Exchange Medium. Anal. Chem. 77 (2005) 5750-5754
22. Winston R.L., and Fitzgerald M.C. Concentration and Desalting of Protein Samples for Mass Spectrometry Analysis. Anal. Biochem. 262 (1998) 83-85