Combination of PTEN and γ-Ionizing Radiation Enhances Cell Death and G2/M Arrest Through Regulation of AKT Activity and p21 Induction in Non-Small-Cell Lung Cancer Cells

International Journal of Radiation Oncology Biology Physics

Volumn 70, Issue 5, 2008, Pages 1552-1560

  Park, Jong Kuk ^a   Jung, Hae Yun ^a   Park, Seon Ho ^a   Kang, Seung Yi ^a   Yi, Mi Rang ^a   Um, Hong Duck ^a   Hong, Sung Hee ^a  

^a Korea Institute of Radiation and Medical Science   (South Korea)

Author keywords

Radiation; G2 M arrest; p21; PI3K AKT; PTEN

Indexed keywords

CELL DEATH; CHROMOSOMES; ONCOLOGY;

CANCER CELLS; LUNG CANCER; PTEN TRANSFECTANTS;

IONIZING RADIATION;

BETA ACTIN; CYCLIN B; CYCLIN DEPENDENT KINASE 1; PHOSPHATIDYLINOSITOL 3,4,5 TRISPHOSPHATE 3 PHOSPHATASE; PROTEIN KINASE B; PROTEIN P21; PROTEIN P27; SMALL INTERFERING RNA;

ARTICLE; CANCER CELL CULTURE; CELL COUNT; CELL CYCLE ARREST; CELL CYCLE G1 PHASE; CELL CYCLE G2 PHASE; CELL CYCLE M PHASE; CELL CYCLE S PHASE; CELL DEATH; CELL PROLIFERATION; CONTROLLED STUDY; ENZYME ASSAY; GAMMA RADIATION; HUMAN; HUMAN CELL; IONIZING RADIATION; LUNG NON SMALL CELL CANCER; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION;

1-PHOSPHATIDYLINOSITOL 3-KINASE; CARCINOMA, NON-SMALL-CELL LUNG; CDC2 PROTEIN KINASE; CELL CYCLE; CELL CYCLE PROTEINS; CELL DEATH; CELL LINE, TUMOR; CELL SURVIVAL; CHROMONES; CYCLIN-DEPENDENT KINASE INHIBITOR P21; ENZYME INHIBITORS; G2 PHASE; GAMMA RAYS; HUMANS; LUNG NEOPLASMS; MORPHOLINES; NEOPLASM PROTEINS; PHOSPHORYLATION; PROTO-ONCOGENE PROTEINS C-AKT; PTEN PHOSPHOHYDROLASE; TRANSFECTION;

EID: 40949160953     PISSN: 03603016     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijrobp.2007.11.069     Document Type: Article

References (41)

1. Kim H.-K. Lung cancer in Korea. Cancer Res Treat 34 (2002) 1-2
2. Martini B. [Lung cancer-epidemiology, prognosis and therapy]. Med Monatsschr Pharm 29 (2006) 217-221
3. Yamamoto N., Nishimura Y., Nakagawa K., et al. Phase I/II study of weekly docetaxel dose escalation in combination with fixed weekly cisplatin and concurrent thoracic radiotherapy in locally advanced non-small cell lung cancer. Cancer Chemother Pharmacol 58 (2006) 285-291
4. McKay M.J. Guidelines for radical radiotherapy in skin cancer. Australas Radiol 50 (2006) 286-287
5. Bellon J.R., Katz A., and Taghian A. Radiation therapy for breast cancer. Hematol Oncol Clin North Am 20 (2006) 239-257
6. Hawkins M.A., and Dawson L.A. Radiation therapy for hepatocellular carcinoma: From palliation to cure. Cancer 106 (2006) 1653-1663
7. McGinn C.J., Shewach D.S., and Lawrence T.S. Radiosensitizing nucleosides. J Natl Cancer Inst 88 (1996) 1193-1203
8. Stratford I.J. Concepts and developments in radiosensitization of mammalian cells. Int J Radiat Oncol Biol Phys 22 (1992) 529-532
9. Hirose T., Mizutani Y., Ohmori T., et al. The combination of cisplatin and vinorelbine with concurrent thoracic radiation therapy for locally advanced stage IIIA or IIIB non-small-cell lung cancer. Cancer Chemother Pharmacol 58 (2006) 361-367
10. Albert J.M., Kim K.W., Cao C., et al. Targeting the Akt/mammalian target of rapamycin pathway for radiosensitization of breast cancer. Mol Cancer Ther 5 (2006) 1183-1189
11. Choi E.K., Ji I.M., Lee S.R., et al. Radiosensitization of tumor cells by modulation of ATM kinase. Int J Radiat Biol 82 (2006) 277-283
12. Lee C.M., Fuhrman C.B., Planelles V., et al. Phosphatidylinositol 3-kinase inhibition by LY294002 radiosensitizes human cervical cancer cell lines. Clin Cancer Res 12 (2006) 250-256
13. Myers M.P., Pass I., Batty I.H., et al. The lipid phosphatase activity of PTEN is critical for its tumor supressor function. Proc Natl Acad Sci U S A 95 (1998) 13513-13518
14. Chu E.C., and Tarnawski A.S. PTEN regulatory functions in tumor suppression and cell biology. Med Sci Monit 10 (2004) RA235-RA241
15. Kandasamy K., and Srivastava R.K. Role of the phosphatidylinositol 3'-kinase/PTEN/Akt kinase pathway in tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis in non-small cell lung cancer cells. Cancer Res 62 (2002) 4929-4937
16. Liu J.L., Sheng X., Hortobagyi Z.K., et al. Nuclear PTEN-mediated growth suppression is independent of Akt down-regulation. Mol Cell Biol 25 (2005) 6211-6224
17. Pommery N., and Henichart J.P. Involvement of PI3K/Akt pathway in prostate cancer-potential strategies for developing targeted therapies. Mini Rev Med Chem 5 (2005) 1125-1132
18. Li J., Yen C., Liaw D., et al. PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer. Science 275 (1997) 1943-1947
19. Steck P.A., Pershouse M.A., Jasser S.A., et al. Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers. Nat Genet 15 (1997) 356-362
20. Gottschalk A.R., Basila D., Wong M., et al. p27Kip1 is required for PTEN-induced G1 growth arrest. Cancer Res 61 (2001) 2105-2111
21. Liang J., Zubovitz J., Petrocelli T., et al. PKB/Akt phosphorylates p27, impairs nuclear import of p27 and opposes p27-mediated G1 arrest. Nat Med 8 (2002) 1153-1160
22. Lee S.R., Park J.H., Park E.K., et al. Akt-induced promotion of cell-cycle progression at G2/M phase involves upregulation of NF-Y binding activity in PC12 cells. J Cell Physiol 205 (2005) 270-277
23. Tran H., Brunet A., Grenier J.M., et al. DNA repair pathway stimulated by the forkhead transcription factor FOXO3a through the Gadd45 protein. Science 296 (2002) 530-534
24. Sanchez Y., Wong C., Thoma R.S., et al. Conservation of the Chk1 checkpoint pathway in mammals: Linkage of DNA damage to Cdk regulation through Cdc25. Science 277 (1997) 1497-1501
25. Shtivelman E., Sussman J., and Stokoe D. A role for PI 3-kinase and PKB activity in the G2/M phase of the cell cycle. Curr Biol 12 (2002) 919-924
26. Maehama T., and Dixon J.E. The tumor suppressor, PTEN/MMAC1, dephosphorylates the lipid second messenger, phosphatidylinositol 3,4,5-trisphosphate. J Biol Chem 273 (1998) 13375-13378
27. Ramaswamy S., Nakamura N., Vazquez F., et al. Regulation of G1 progression by the PTEN tumor suppressor protein is linked to inhibition of the phosphatidylinositol 3-kinase/Akt pathway. Proc Natl Acad Sci U S A 96 (1999) 2110-2115
28. Hartmann W., Digon-Sontgerath B., Koch A., et al. Phosphatidylinositol 3'-kinase/AKT signaling is activated in medulloblastoma cell proliferation and is associated with reduced expression of PTEN. Clin Cancer Res 12 (2006) 3019-3027
29. Bertram J., Peacock J.W., Tan C., et al. Inhibition of the phosphatidylinositol 3'-kinase pathway promotes autocrine Fas-induced death of phosphatase and tensin homologue-deficient prostate cancer cells. Cancer Res 66 (2006) 4781-4788
30. Vogelmann R., Nguyen-Tat M.D., Giehl K., et al. TGFbeta-induced downregulation of E-cadherin-based cell-cell adhesion depends on PI3-kinase and PTEN. J Cell Sci 118 (2005) 4901-4912
31. Zhou M., Gu L., Findley H.W., et al. PTEN reverses MDM2-mediated chemotherapy resistance by interacting with p53 in acute lymphoblastic leukemia cells. Cancer Res 63 (2003) 6357-6362
32. Chu E.C., Chai J., and Tarnawski A.S. NSAIDs activate PTEN and other phosphatases in human colon cancer cells: Novel mechanism for chemopreventive action of NSAIDs. Biochem Biophys Res Commun 320 (2004) 875-879
33. Kandel E.S., Skeen J., Majewski N., et al. Activation of Akt/protein kinase B overcomes a G(2)/m cell cycle checkpoint induced by DNA damage. Mol Cell Biol 22 (2002) 7831-7841
34. Perez-Tenorio G., Berglund F., Esguerra Merca A., et al. Cytoplasmic p21WAF1/CIP1 correlates with Akt activation and poor response to tamoxifen in breast cancer. Int J Oncol 28 (2006) 1031-1042
35. Lu S., Ren C., Liu Y., et al. PI3K-Akt signaling is involved in the regulation of p21(WAF/CIP) expression and androgen-independent growth in prostate cancer cells. Int J Oncol 28 (2006) 245-251
36. Boulaire J., Fotedar A., and Fotedar R. The functions of the cdk-cyclin kinase inhibitor p21WAF1. Pathol Biol 48 (2000) 190-202
37. Bunz F., Dutriaux A., Lengauer C., et al. Requirement for p53 and p21 to sustain G2 arrest after DNA damage. Science 282 (1998) 1497-1501
38. Lee B., Kim C.H., and Moon S.K. Honokiol causes the p21WAF1-mediated G(1)-phase arrest of the cell cycle through inducing p38 mitogen activated protein kinase in vascular smooth muscle cells. FEBS Lett 580 (2006) 5177-5184
39. Shen G., Xu C., Chen C., et al. p53-Independent G1 cell cycle arrest of human colon carcinoma cells HT-29 by sulforaphane is associated with induction of p21CIP1 and inhibition of expression of cyclin D1. Cancer Chemother Pharmacol 57 (2006) 317-327
40. Faivre S., Djelloul S., and Raymond E. New paradigms in anticancer therapy: Targeting multiple signaling pathways with kinase inhibitors. Semin Oncol 33 (2006) 407-420
41. Herr I., and Debatin K.M. Cellular stress response and apoptosis in cancer therapy. Blood 98 (2001) 2603-2614