Detection of Cryptosporidium parvum oocysts by dot-blotting using monoclonal antibodies to Cryptosporidium parvum virus 40-kDa capsid protein

Journal of Parasitology

Volumn 94, Issue 1, 2008, Pages 94-98

  Jenkins, Mark C ^a   O'Brien, Celia N ^a   Trout, James M ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CAPSID PROTEIN; MONOCLONAL ANTIBODY;

ANTIBODY; ANTIGEN; DETECTION METHOD; GENETIC ANALYSIS; IMMUNOASSAY; PROTEIN; PROTOZOAN; RECOMBINATION; RODENT; VIRUS;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; CRYPTOSPORIDIUM PARVUM; CYTOPLASM; DOT HYBRIDIZATION; IMMUNIZATION; IMMUNOBLOTTING; IMMUNOFLUORESCENCE; MOUSE; NONHUMAN; OOCYST; POLYACRYLAMIDE GEL ELECTROPHORESIS;

ANIMALS; ANTIBODIES, MONOCLONAL; CAPSID PROTEINS; CATTLE; CRYPTOSPORIDIUM PARVUM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLUORESCENT ANTIBODY TECHNIQUE; HYBRIDOMAS; IMMUNOBLOTTING; MICE; MICE, INBRED BALB C; OOCYSTS; RECOMBINANT PROTEINS; RNA VIRUSES; SENSITIVITY AND SPECIFICITY; SYMBIOSIS;

CRYPTOSPORIDIUM PARVUM; MIRIDAE; MUS;

EID: 40949156286     PISSN: 00223395     EISSN: None     Source Type: Journal    
DOI: 10.1645/GE-1313.1     Document Type: Article

References (32)

1. BENDER, F. C., J. C. WHITBECK, H. LOU, G. H. COHEN, AND R. J. EISENBERG. 2005. Herpes simplex virus glycoprotein B binds to cell surfaces independently of heparin sulfate and blocks virus entry. Journal of Virology 79: 11588-11597.
2. DIGIORGIO, C. L., D. A. GONZALEZ, AND C. C. HUITT. 2002. Cryptosporidium and Giardia recoveries in natural waters by using environmental protection agency method 1623. Applied and Environmental Microbiology 68: 5952-5955.
3. DI GIOVANNI, G. D., F. H. HASHEMI, N. J. SHAW, F. A. ABRAMS, M. W. LECHEVALLIER, AND M. ABBASZADEGAN. 1999. Detection of infectious Cryptosporidium parvum oocysts in surface and filter backwash water samples by immunomagnetic separation and integrated cell culture-PCR. Applied and Environmental Microbiology 65: 3427-3432.
4. FERRARI, B. C., G. VESEY, K. A. DAVIS, M. GAUCI, AND D. VEAL. 2000. A novel two-color flow cytometric assay for the detection of Cryptosporidium in environmental water samples. Cytometry 41: 216-222.
5. GARCIA, L. S., A. C. SHUM, AND D. A. BRUCKNER. 1992. Evaluation of a new monoclonal antibody combination reagent for direct fluorescence detection of Giardia cysts and Cryptosporidium oocysts in human fecal specimens. Journal of Clinical Microbiology 30: 3255-3257.
6. GRANDE, A., C. COSTAS, AND J. BENAVENTE. 2002. Subunit composition and conformational stability of the oligomeric form of the avian reovirus cell-attachment protein sigmaC. Journal of General Virology 83: 131-139.
7. HALLIER-SOULIER, S., AND E. GUILLOT. 2000. Detection of cryptosporidia and Cryptosporidium parvum oocysts in environmental water samples by immunomagnetic separation-polymerase chain reaction. Journal of Applied Microbiology 89: 5-10.
8. HU, J., Y. FENG, S. L. ONG, W. J. NG, L. SONG, X. TAN, AND X. CHU. 2004. Improvement of recoveries for the determination of protozoa Cryptosporidium and Giardia in water using method 1623. Journal of Microbiological Methods 58: 321-325.
9. KNIEL, K. E., J. A. HIGGINS, J. M. TROUT, R. FAYER, AND M. C. JENKINS. 2004. Characterization and potential use of a Cryptosporidium parvum virus (CPV) antigen for detecting C. parvum oocysts. Journal of Microbiological Methods 58: 189-195.
10. KOZWICH, D., K. A. JOHANSEN, K. LANDAU, C. A. ROEHL, S. WORONOFF, AND P. A. ROEHL. 2000. Development of a novel, rapid integrated Cryptosporidium parvum detection assay. Applied and Environmental Microbiology 66: 2711-2717.
11. KUCZYNSKA, E., D. G. BOYER, AND D. R. SHELTON. 2003. Comparison of immunofluorescence assay and immunomagnetic electrochemiluminescence in detection of Cryptosporidium parvum oocysts in karst water samples. Journal of Microbiological Methods 53: 17-26.
12. LAEMMLI, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
13. LECHEVALLIER, M. W., G. D. DI GIOVANNI, J. L. CLANCY, Z. BUKHARI, S. BUKHARI, J. S. ROSEN, J. SOBRINHO, AND M. M. FREY. 2003. Comparison of method 1623 and cell culture-PCR for detection of Cryptosporidium spp. in source waters. Applied and Environmental Microbiology 69: 971-979.
14. LEE, Y. M., P. W. JOHNSON, J. L. CALL, M. J. ARROWOOD, B. W. FURNESS, S. C. PICHETTE, K. K. GRADY, P. REEH, L. MITCHELL, D. B ERGMIRESWEAT, ET AL. 2001. Development and application of a quantitative, specific assay for Cryptosporidium parvum oocyst detection in high-turbidity environmental samples. American Journal of Tropical Medicine and Hygiene 65: 1-9.
15. LEMOS, V., T. K. GRACZYK, M. ALVES, M. L. LOBO, M. C. SOUSA, F. ANTUNES, AND O. MATOS. 2005. Identification and determination of the viability of Giardia lamblia cysts and Cryptosporidium parvum and Cryptosporidium hominis oocysts in human fecal and water supply samples by fluorescent in situ hybridization (FISH) and monoclonal antibodies. Parasitology Research 98: 48-53.
16. LINDQUIST, H. D., M. WARE, R. E. STETLER, L. WYMER, AND F. W. SCHAEFER, III. 2001. A comparison of four fluorescent antibody-based methods for purifying, detecting, and confirming Cryptosporidium parvum in surface waters. Journal of Parasitology 87: 1124-1131.
17. MARTINEZ-COSTAS, J., A. GRANDE, R. VARELA, C. GARCIA-MARTINEZ, AND J. BENAVENTE. 1997. Protein architecture of avian reovirus S1133 and identification of the cell attachment protein. Journal of Virology 71: 59-64.
18. MOORE, A. G., G. VESEY, A. CHAMPION, P. SCANDIZZO, D. DEERE, D. VEAL, AND K. L. WILLIAMS. 1998. Viable Cryptosporidium parvum oocysts exposed to chlorine or other oxidising conditions may lack identifying epitopes. International Journal for Parasitology 28: 1205-1212.
19. MORGAN, U. M., AND R. C. THOMPSON. 1998. PCR detection of Cryptosporidium: The way forward? Parasitology Today 14: 241-245.
20. MYODA, S. P., AND C. P. HUANG. 2001. A microscope system with a dual-band filter for the simultaneous enumeration of Cryptosporidium parvum oocysts and sporozoites. Water Research 35: 4231-4236.
21. O'BRIEN, C. N., AND M. C. JENKINS. 2007. A rapid method for producing highly purified Cryptosporidium parvum oocysts. Journal of Parasitology 93: 434-436.
22. QUINTERO-BETANCOURT, W., E. R. PEELE, AND J. B. ROSE. 2002. Cryptosporidium parvum and Cyclospora cayetanensis: A review of laboratory methods for detection of these waterborne parasites. Journal of Microbiological Methods 49: 209-224.
23. SIMMONS, O. D., III, M. D. SOBSEY, C. D. HEANEY, F. W. SCHAEFER, III, AND D. S. FRANCY. 2001. Concentration and detection of Cryptosporidium oocysts in surface water samples by method 1622 using ultrafiltration and capsule filtration. Applied and Environmental Microbiology 67: 1123-1127.
24. SMITH, H. V., B. M. CAMPBELL, C. A. PATON, AND R. A. NICHOLS. 2002. Significance of enhanced morphological detection of Cryptosporidium sp. oocysts in water concentrates determined by using 4′,6′-diamidino-2-phenylindole and immunofluorescence microscopy. Applied and Environmental Microbiology 68: 5198-5201.
25. STURBAUM, G. D., P. T. KLONICKI, M. M. MARSHALL, B. H. JOST, B. L. CLAY, AND C. R. STERLING. 2002. Immunomagnetic separation (IMS)-fluorescent antibody detection and IMS-PCR detection of seeded Cryptosporidium parvum oocysts in natural waters and their limitations. Applied and Environmental Microbiology 68: 2991-2996.
26. TAGUCHI, T., A. ARAKAKI, H. TAKEYAMA, S. HARAGUCHI, M. YOSHINO, M. KANEKO, Y. ISHIMORI, AND T. MATSUNAGA. 2007. Detection of Cryptosporidium parvum oocysts using a microfluidic device equipped with the SUS micromesh and FITC-labeled antibody. Biotechnology and Bioengineering 96: 272-280.
27. WARE, M. W., L. WYMER, H. D. LINDQUIST, AND F. W. SCHAEFER, III. 2003. Evaluation of an alternative IMS dissociation procedure for use with Method 1622: Detection of Cryptosporidium in water. Journal of Microbiological Methods 55: 575-583.
28. WEIR, C., G. VESEY, M. SLADE, B. FERRARI, D. A. VEAL, AND K. WILLIAMS. 2000. An immunoglobulin G1 monoclonal antibody highly specific to the wall of Cryptosporidium oocysts. Clinical and Diagnostic Laboratory Immunology 7: 745-750.
29. WILLINGHAM, M. C. 1994. Fluorescence labeling of intracellular antigens of attached or suspended tissue culture cells. In Immunocytochemical methods and protocols. Methods in molecular biology, Vol. 34, L. C. Javois (ed.). Humana Press, Totowa, New Jersey, p. 131-142.
30. XIAO, L., A. A. LAL, AND J. JIANG. 2004. Detection and differentiation of Cryptosporidium oocysts in water by PCR-RFLR Methods in Molecular Biology 268: 163-176.
31. YOKOYAMA, W. M. 1994. Production of monoclonal antibodies. In Current protocols in immunology, J. E. Coligan, A. M. Kruisbeek, D. H. Margulies, E. M. Shevach, and W. Strober (eds.). Wiley, New York, New York, p. 1:2.5.1-2.5.12.
32. ZUCKERMAN, U., AND S. TZIPORI. 2006. Portable continuous flow centrifugation and method 1623 for monitoring of waterborne protozoa from large volumes of various water matrices. Journal of Applied Microbiology 100: 1220-1227.