Efficient long-term and high-yielded production of a recombinant proteoglycan in eukaryotic HEK293 cells using a membrane-based bioreactor

Biochemical and Biophysical Research Communications

Volumn 369, Issue 2, 2008, Pages 297-302

  Adam, Estelle ^a,b   Sarrazin, Stéphane ^b,c   Landolfi, Cindy ^b   Motte, Vincent ^b   Lortat Jacob, Hugues ^c   Lassalle, Philippe ^a   Delehedde, Maryse ^a,b  

^a INSTITUT PASTEUR DE LILLE   (France)

^b Endotis Pharma ^*  (France)

^c CEA GRENOBLE   (France)

Author keywords

Bioreactor CELLine ; Endocan; Glycosylation; High density cell culture; Proteoglycan

Indexed keywords

ENDOCAN; MONOCLONAL ANTIBODY; PROTEOGLYCAN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BIOENGINEERING; BIOREACTOR; CELL CULTURE; CELL GROWTH; CELL STRAIN HEK293; CELL VIABILITY; CONTROLLED STUDY; ELECTROPHORESIS; EUKARYOTIC CELL; GLYCOSYLATION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION;

BIOREACTORS; CELL CULTURE TECHNIQUES; CELL LINE; HUMANS; KIDNEY; MEMBRANES, ARTIFICIAL; NEOPLASM PROTEINS; PROTEIN ENGINEERING; PROTEOGLYCANS; RECOMBINANT PROTEINS;

EUKARYOTA;

EID: 40949117560     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2008.01.141     Document Type: Article

References (21)

1. Wurm F.M. Production of recombinant protein therapeutics in cultivated mammalian cells. Nat. Biotechnol. 22 (2004) 1393-1398
2. Ozturk S.S., and Hu W. Cell culture technology-an overview. In: Ozturk S.S., and Hu W. (Eds). Cell Culture Technology for Pharmaceutical and Cell-Based Therapies (2006), Taylor & Francis, New York 1-14
3. Delehedde M., Sarrazin S., Adam E., Motte V., and Vanpouille C. Proteoglycans and glycosaminoglycans: complex molecules with modulating activity. In: Delehedde M. (Ed). New Developments in Therapeutic Glycomics (2006), Research Signpost, Kerala 1-13
4. Ohtsubo K., and Marth J.D. Glycosylation in cellular mechanisms of health and disease. Cell 126 (2006) 855-867
5. Jelkmann W. Erythropoietin after a century of research: younger than ever. Eur J. Haematol. 78 (2007) 183-205
6. Harcum S. Protein glycosylation. In: Ozturk S., and Hu W. (Eds). Cell Culture Technology for Pharmaceutical and Cell-Based Therapies (2006), Taylor & Francis, New York 113-154
7. Zucca A., Brizzi S., Riccioni R., Azzara A., Ghimenti M., and Carulli G. Glycosylated and nonglycosylated recombinant human granulocyte colony-stimulating factor differently modifies actin polymerization in neutrophils. Clin. Ter. 157 (2006) 19-24
8. Trebak M., Chong J.M., Herlyn D., and Speicher D.W. Efficient laboratory-scale production of monoclonal antibodies using membrane-based high-density cell culture technology. J. Immunol. Methods 230 (1999) 59-70
9. Bruce M.P., Boyd V., Duch C., and White J.R. Dialysis-based bioreactor systems for the production of monoclonal antibodies-alternatives to ascites production in mice. J. Immunol. Methods 264 (2002) 59-68
10. Nagel A., Koch S., Valley U., Emmrich F., and Marx U. Membrane-based cell culture systems-an alternative to in vivo production of monoclonal antibodies. Dev. Biol. Stand 101 (1999) 57-64
11. Lassalle P., Molet S., Janin A., Heyden J.V., Tavernier J., Fiers W., Devos R., and Tonnel A.B. ESM-1 is a novel human endothelial cell-specific molecule expressed in lung and regulated by cytokines. J. Biol. Chem. 271 (1996) 20458-20464
12. Bechard D., Gentina T., Delehedde M., Scherpereel A., Lyon M., Aumercier M., Vazeux R., Richet C., Degand P., Jude B., Janin A., Fernig D.G., Tonnel A.B., and Lassalle P. Endocan is a novel chondroitin sulfate/dermatan sulfate proteoglycan that promotes hepatocyte growth factor/scatter factor mitogenic activity. J. Biol. Chem. 276 (2001) 48341-48349
13. Sarrazin S., Adam E., Lyon M., Depontieu F., Motte V., Landolfi C., Lortat-Jacob H., Bechard D., Lassalle P., and Delehedde M. Endocan or endothelial cell specific molecule-1 (ESM-1): a potential novel endothelial cell marker and a new target for cancer therapy. Biochim. Biophys Acta 1765 (2006) 25-37
14. Scherpereel A., Gentina T., Grigoriu B., Senechal S., Janin A., Tsicopoulos A., Plenat F., Bechard D., Tonnel A.B., and Lassalle P. Overexpression of endocan induces tumor formation. Cancer Res. 63 (2003) 6084-6089
15. Scherpereel A., Depontieu F., Grigoriu B., Cavestri B., Tsicopoulos A., Gentina T., Jourdain M., Pugin J., Tonnel A.B., and Lassalle P. Endocan, a new endothelial marker in human sepsis. Crit. Care Med. 34 (2006) 532-537
16. Bechard D., Meignin V., Scherpereel A., Oudin S., Kervoaze G., Bertheau P., Janin A., Tonnel A., and Lassalle P. Characterization of the secreted form of endothelial-cell-specific molecule 1 by specific monoclonal antibodies. J. Vasc. Res. 37 (2000) 417-425
17. Freshney R.I. Culture of Animal Cells: A Manual of Basic Technique. fifth ed. (2005), Wiley-Liss, Wilmington, DE
18. Docagne F., Colloc'h N., Bougueret V., Page M., Paput J., Tripier M., Dutartre P., MacKenzie E.T., Buisson A., Komesli S., and Vivien D. A soluble transforming growth factor-beta (TGF-beta) type I receptor mimics TGF-beta responses. J. Biol. Chem. 276 (2001) 46243-46250
19. Aitkenhead M., Wang S.J., Nakatsu M.N., Mestas J., Heard C., and Hughes C.C. Identification of endothelial cell genes expressed in an in vitro model of angiogenesis: induction of ESM-1, (beta)ig-h3, and NrCAM. Microvasc. Res. 63 (2002) 159-171
20. Abid M.R., Yi X., Yano K., Shih S.C., and Aird W.C. Vascular endocan is preferentially expressed in tumor endothelium. Microvasc. Res. 72 (2006) 136-145
21. Grigoriu B.D., Depontieu F., Scherpereel A., Gourcerol D., Devos P., Ouatas T., Lafitte J.J., Copin M.C., Tonnel A.B., and Lassalle P. Endocan expression and relationship with survival in human non-small cell lung cancer. Clin. Cancer Res. 12 (2006) 4575-4582