High-resolution computer simulation of the dynamics of isoelectric focusing: In quest of more realistic input parameters for carrier ampholytes

Electrophoresis

Volumn 29, Issue 5, 2008, Pages 1036-1047

  Mosher, Richard A ^a   Thormann, Wolfgang ^b  

^a RAM Software Solutions   (United States)

^b UNIVERSITY OF BERN   (Switzerland)

Author keywords

Conductance gap; Electrophoretic mobility; IEF; pKa value; Simulation

Indexed keywords

AMPHOLYTES; ANIONIC SPECIES; CARRIER AMPHOLYTES; CATIONIC SPECIES; CONDUCTANCE GAP; HIGH RESOLUTION; IEF; ISOELECTRIC FOCUSING; PKA VALUE; SIMULATION;

ELECTROPHORETIC MOBILITY;

AMPHOLYTE; ANION; CATION; DYE;

ACCURACY; ARTICLE; COMPUTER SIMULATION; CONDUCTANCE; ELECTRIC FIELD; ELECTROCHEMISTRY; ELECTROPHORESIS; ELECTROPHORETIC MOBILITY; IMAGE ANALYSIS; ISOELECTRIC FOCUSING; PH; PKA;

EID: 40949097367     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.200700453     Document Type: Article

References (34)

1. Rilbe, H., Ann. N. Y. Acad. Sci. 1973, 209, 11-22.
2. Righetti, R G., Isoelectric Focusing: Theory, Methodology and Applications, Elsevier, Amsterdam 1983.
3. Mosher, R. A., Seville, D. A., Thormann, W., The Dynamics of Electrophoresis, VCH Publishers, Weinheim 1992.
4. Hjertén, S., in: Grossman, R D., Colburn, J. C. (Eds.), Capillary Electrophoresis, Theory and Practice, Academic Press, San Diego, CA 1992, pp. 191-214.
5. Righetti, R G., Stoyanov, A. V., Zhukov, M. Y., The Proteome Revisited, Theory and Practice of all Relevant Electrophoretic Steps, Elsevier, Amsterdam, The Netherlands 2001.
6. Garrin, D., Ahuja, S. (Eds.), Handbook of Isoelectric Focusing and Proteomics, Elsevier, Amsterdam, The Netherlands 2005.
7. Thormann, W., Mosher, R. A., Adv. Electrophor. 1988, 2, 45-108.
8. Mosher, R. A., Thomann, W., in: Mosher, R. A., Saville, D. A., Thormann, W., The Dynamics of Electrophoresis, VCH Publishers, Weinheim 1992, pp. 163--231.
9. Schimeo, K., Jpn. J. Electrophor. 1994, 36, 221-225.
10. Steinmann, L., Mosher, R. A., Thormann, W., J. Chromatogr. A 1996, 756, 219-232.
11. Thormann, W., Zhang, C.-X., Caslavska, J., Gebauer, P., Mosher, R. A., Anal. Chem. 1998, 70, 549-562.
12. Thormann, W., Calavska, J., Mosher, R. A., J. Chromatogr. A 2007, 1155, 154-163.
13. Mao, Q., Pawliszyn, J., Thormann, W., Anal. Chem. 2000, 72, 5493-5502.
14. Mosher, R. A., Thormann, W., Electrophoresis 2002, 23, 1803-1814.
15. Thormann, W., Huang, T. M., Pawliszyn, J., Mosher, R. A., Electrophoresis 2004, 25, 324-337.
16. Thormann, W., Mosher, R. A., Electrophoresis 2006, 27, 968-983.
17. Sounart, T. L., Safier, R A., Baygents, J. C., in: Garrin, D., Ahuja, S. (Eds.), Handbook of Isoelectric Focusing and Proteomics, Elsevier, Amsterdam, The Netherlands 2005, pp. 41-68.
18. Shim, J., Dutta, R, Ivory, C. F., Electrophoresis 2007, 28, 572-586.
19. Thormann, W., Egen, N. B., Mosher, R. A., Bier, M., J. Biochem. Biophys. Methods 1985, 11, 287-293.
20. Sebastiano, R., Simó, C., Mendieta, M. E., Antonioli, P. et al., Electrophoresis 2006, 27, 3919-3934.
21. Simó, C., Mendieta, M. E., Antonioll, R, Sebastiano, R. et al., Electrophoresis 2006, 27, 4849-4858.
22. Simó, C., Mendieta, M. E., Antonioli, R, Sebastiano, R. et al., Electrophoresis 2007, 28, 715-723.
23. Simó, C., Cittero, A., Righetti, P. G., Electrophoresis 2007, 28, 1488-1494.
24. Bier, M., Palusinski, O. A., Mosher, R. A., Saville, D. A., Science 1983, 219, 1281-1287.
25. Saville, D. A., Palusinski, O. A., AlChE J. 1986, 32, 207-214.
26. Palusinski, O. A., Graham, A., Mosher, R. A., Bier, M., Saville, D. A., AIChE J. 1986, 32, 215-223.
27. Hruška, V., Jaroš, M., Gaš, B., Electrophoresis 2006, 27, 984-991.
28. Edward, J. T., Waldron-Edward, D., J. Chromatogr. 1965, 20, 563-571.
29. Bier, M., Mosher, R. A., Palusinski, O. A., J. Chromatogr. 1981, 211, 313-335.
30. Pospichal, J., Gebauer, R, Bodek, P., Chem. Rev. 1989, 89, 419-430.
31. Hirokawa, T., Kiso, Y., Gaš B., Zusková, I., Vacík J., J. Chromatogr. 1993, 628, 283-303.
32. Hirokawa, T., database in Peakmaster 5.1 software package, www.natur.cuni.cz/gas.
33. Hruška, V., Gaš, B., SIMUL5: Electrophoretic Simulation Software for Method Development and Education, poster P1, ITP 2006, 15th International Symposium on Capillary Electroseparation Techniques, August 28-30, 2006, Paris, France.
34. Mosher, R. A., Thormann, W., Bier, M., J. Chromatogn 1986, 351, 31-38.