Mutational analysis of histidine residues in the human proton-coupled amino acid transporter PAT1

Biochimica et Biophysica Acta - Biomembranes

Volumn 1778, Issue 4, 2008, Pages 1042-1050

  Metzner, Linda ^a   Natho, Kristin ^a   Zebisch, Katja ^a   Dorn, Madlen ^a   Bosse Doenecke, Eva ^a   Ganapathy, Vadivel ^b   Brandsch, Matthias ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^b MEDICAL COLLEGE OF GEORGIA   (United States)

Author keywords

Amino acid transport; Drug delivery; Histidine residues; PAT1; Proline; Site directed mutagenesis

Indexed keywords

AMINO ACID TRANSPORTER; HISTIDINE; PROLINE; PROTON COUPLED AMINO ACID TRANSPORTER 1; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BIOTINYLATION; CATALYSIS; CONFOCAL MICROSCOPY; CONTROLLED STUDY; HUMAN; HUMAN CELL; IMMUNOBLOTTING; MUTATIONAL ANALYSIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; WILD TYPE;

AMINO ACID SEQUENCE; AMINO ACID TRANSPORT SYSTEMS; BIOLOGICAL TRANSPORT; CELLS, CULTURED; CONSERVED SEQUENCE; DNA MUTATIONAL ANALYSIS; DNA, COMPLEMENTARY; FLUORESCENT ANTIBODY TECHNIQUE; HISTIDINE; HUMANS; IMMUNOBLOTTING; MOLECULAR SEQUENCE DATA; MUTANT PROTEINS; PROLINE; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY; SYMPORTERS;

ANIMALIA;

EID: 40949093028     PISSN: 00052736     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbamem.2007.12.026     Document Type: Article

References (25)

1. Sagne C., Agulhon C., Ravassard P., Darmon M., Hamon M., El Mestikawy S., Gasnier B., and Giros B. Identification and characterization of a lysosomal transporter for small neutral amino acids. Proc. Natl. Acad. Sci. U. S. A. 98 (2001) 7206-7211
2. Boll M., Foltz M., Rubio-Aliaga I., Kottra G., and Daniel H. Functional characterization of two novel mammalian electrogenic proton-dependent amino acid cotransporters. J. Biol. Chem. 277 (2002) 22966-22973
3. Chen Z., Fei Y.L., Anderson C.M., Wake K.A., Miyauchi S., Huang W., Thwaites D.T., and Ganapathy V. Structure, function and immunolocalization of a proton-coupled amino acid transporter (hPAT1) in the human intestinal cell line Caco-2. J. Physiol. 546 (2003) 349-361
4. +-independent) proline transport in human intestinal (Caco-2) epithelial cell monolayers. FEBS Lett. 333 (1993) 78-82
5. Ranaldi G., Islam K., and Sambuy Y. d-cycloserine uses an active transport mechanism in the human intestinal cell line Caco 2. Antimicrob. Agents Chemother. 38 (1994) 1239-1245
6. +/Amino acid transporter 1 (PAT1) is the imino acid carrier: An intestinal nutrient/drug transporter in human and rat. Gastroenterology 127 (2004) 1410-1422
7. Metzner L., Kalbitz J., and Brandsch M. Transport of pharmacologically active proline derivatives by the human proton-coupled amino acid transporter hPAT1. J. Pharmacol. Exp. Ther. 309 (2004) 28-35
8. Brandsch M. Transport of l-proline, l-proline-containing peptides and related drugs at mammalian epithelial cell membranes. Amino Acids 31 (2006) 119-136
9. Thwaites D.T., and Anderson C.M. Deciphering the mechanisms of intestinal imino (and amino) acid transport: the redemption of SLC36A1. Biochim. Biophys. Acta 1768 (2007) 179-197
10. + and substrate transport by the proton-dependent amino acid symporter PAT1. Biochem. J. 386 (2005) 607-616
11. +-coupled nutrient, micronutrient and drug transporters in the mammalian small intestine. Exp. Physiol. 92 (2007) 603-619
12. Padan E., Patel L., and Kaback H.R. Effect of diethylpyrocarbonate on lactose/proton symport in Escherichia coli membrane vesicles. Proc. Natl. Acad. Sci. 76 (1979) 6221-6225
13. +/peptide cotransport systems in intestine (PEPT 1) and kidney (PEPT 2). Biochim. Biophys. Acta 1324 (1997) 251-262
14. +/peptide cotransporters PEPT1 and PEPT2. Biochemistry 39 (1997) 452-460
15. Said H.M., and Mohammadkhani R. Folate transport in intestinal brush border membrane: involvement of essential histidine residue(s). Biochem. J. 290 (1993) 237-240
16. Trosper T.L., and Philipson K.D. Functional characteristics of the cardiac sarcolemmal monocarboxylate transporter. J. Membr. Biol. 112 (1989) 15-23
17. Rahman B., Schneider H.P., Bröer A., Deitmer J.W., and Bröer S. Helix 8 and helix 10 are involved in substrate recognition in the rat monocarboxylate transporter MCT1. Biochemistry 38 (1999) 11577-11584
18. +/amino acid cotransporter PAT1 in Caco-2 cells. Eur. J. Pharm. Biopharm. 63 (2006) 360-364
19. Boll M., Foltz M., Rubio-Aliaga I., and Daniel H. A cluster of proton/amino acid transporter genes in the human and mouse genomes. Genomics 82 (2003) 47-56
20. Blakely R.D., Clark J.A., Rudnick G., and Amara S.G. Vaccinia-T7 RNA polymerase expression system: evaluation for the expression cloning of plasma membrane transporters. Anal. Biochem. 194 (1991) 302-308
21. Staib C., Drexler I., and Sutter G. Construction and isolation of recombinant MVA. Methods Mol. Biol. 269 (2004) 77-100
22. Metzner L., Kottra G., Neubert K., Daniel H., and Brandsch M. Serotonin, l-tryptophan and tryptamine are effective inhibitors of the human amino acid transport system PAT1. FASEB J. 19 (2005) 1468-1473
23. Boll M., Daniel H., and Gasnier B. The SLC36 family: proton-coupled transporters for the absorption of selected amino acids from extracellular and intracellular proteolysis. Pflugers Arch. 447 (2004) 776-779
24. Bröer A., Cavanaugh J.A., Rasko J.E., and Bröer S. The molecular basis of neutral aminoacidurias. Pflugers Arch. 451 (2006) 511-517
25. Miyauchi S., Abbot E.L., Zhuang L., Subramanian R., Ganapathy V., and Thwaites D.T. Isolation and function of the amino acid transporter PAT1 (slc36a1) from rabbit and discrimination between transport via PAT1 and system IMINO in renal brush-border membrane vesicles. Mol. Membr. Biol. 22 (2005) 549-559