Purification of circular YACs from yeast cells for DNA sequencing

Genome

Volumn 51, Issue 2, 2008, Pages 155-158

  Leem, S H ^a   Yoon, Y H ^a   Kim, S I ^b   Larionov, V ^c  

^a Dong A University   (South Korea)

^b Korea Basic Science Institute (KBSI)   (South Korea)

^c NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Circular YAC; GAP sequence; TAR cloning; Unstable clone in E. coli; YAC purification; YAC sequencing

Indexed keywords

CIRCULAR DNA; FUNGAL DNA;

ARTICLE; GENETICS; GENOME; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; METHODOLOGY; MOLECULAR CLONING; SACCHAROMYCES CEREVISIAE;

CLONING, MOLECULAR; DNA, CIRCULAR; DNA, FUNGAL; GENOME, FUNGAL; SACCHAROMYCES CEREVISIAE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 40749085720     PISSN: 08312796     EISSN: None     Source Type: Journal    
DOI: 10.1139/G07-109     Document Type: Article

References (11)

1. Bellané-Chantelot, C., Lacroix, B., Ougen, P., Billault, A., Beaufils, S., Bertrand, S., et al. 1992. Mapping the whole human genome by fingerprinting yeast artificial chromosomes. Cell, 70: 1059-1068.
2. Burke, D.T., Carle, G.F., and Olson, M.V. 1987. Cloning of large segments of exogeneous DNA into yeast by means of artificial chromosome vectors. Science (Washington, D.C.), 236: 806-812. doi:10.1126/science.3033825.
3. Cohen, D., Chumakov, I., and Weissenbach, J. 1993. A first-generation physical map of the human genome. Nature (London), 366: 698-701. doi:10.1038/366698a0.
4. Devenish, R.J., and Newlon, C.S. 1982. Isolation and characterization of yeast ring chromosome III by a method applicable to other circular DNAs. Gene, 18: 277-288. doi:10.1016/0378-1119(82)90166-4.
5. Green, E.D., and Olson, M.V. 1990. Chromosomal region of the cystic fibrosis gene in yeast artificial chromosomes: a model for human genome mapping. Science (Washington, D.C.), 250: 94-98. doi:10.1126/science.2218515.
6. Kouprina, N., and Larionov, V. 1999. Selective isolation of mammalian genes by TAR cloning. In Current protocols in human genetics. Vol. 1. Edited by A.L. Boyle. John Wiley and Sons, Inc., New York. pp. 5.17.1-5.17.21.
7. Kouprina, N., Leem, S.-H., Solomon, G., Ly, A., Koriabine, M., Otstot, J., et al. 2003. Segments missing from the draft human genome sequence can be isolated by TAR cloning in yeast. EMBO Rep. 4: 257-262. doi:10.1038/sj.embor.embor766.
8. Larionov, V., Kouprina, N., Graves, J., Chen, X.N., Korenberg, J.R., and Resnick, M.A. 1996a. Specific cloning of human DNA as yeast artificial chromosomes by transformation-associated recombination. Proc. Natl. Acad. Sci. U.S.A. 93: 491-496. doi:10.1073/pnas.93.1.491.
9. Larionov, V., Kouprina, N., Graves, J., and Resnick, M.A. 1996b. Highly selective isolation of human DNAs from rodent-human hybrid cells as circular yeast artificial chromosomes by transformation-associated recombination cloning. Proc. Natl. Acad. Sci. U.S.A. 93: 13925-13930. doi:10.1073/pnas.93.24.13925.
10. Leem, S.-H., Noskov, V.N., Park, J.E., Kim, S., Larionov, V., and Kouprina, N. 2003. Optimal conditions for selective isolation of genes from complex genomes by transformation-associated recombination cloning. Nucleic Acids Res. 31: 29e. doi:10.1093/nar/gng029.
11. Leem, S.-H., Kouprina, N., Grimwood, J., Kim, J.-H., Mullokandov, M., Yoon, Y.-H., et al. 2004. Closing the gaps on human chromosome 19 revealed genes with a high density of repetitive tandemly arrayed elements. Genome Res. 14: 239-246. doi:10.1101/gr.1929904.