Purification of human plasma fibronectin using immobilized gelatin and Arg affinity chromatography

Nature Protocols

Volumn 3, Issue 3, 2008, Pages 525-533

  Speziale, Pietro ^a   Visai, Livia ^a   Rindi, Simonetta ^a   Di Poto, Antonella ^a  

^a Department of Biochemistry ^*  (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

AGAROSE; ARGININE; FIBRONECTIN; GELATIN; SEPHAROSE;

ADSORPTION; AFFINITY CHROMATOGRAPHY; ALPHA HELIX; ARTICLE; BETA SHEET; ENZYME LINKED IMMUNOSORBENT ASSAY; GEL FILTRATION CHROMATOGRAPHY; HUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE;

ARGININE; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, AGAROSE; FIBRONECTINS; GELATIN; HUMANS;

EID: 40649083363     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2008.12     Document Type: Article

References (17)

1. Hynes, R.O. Fibronectins. Springer Series in Molecular Biology. (Springer-Verlag, New York, 1990).
2. Tamkun, J.W. & Hynes, R.O. Plasma fibronectin is synthesized and secreted by hepatocytes. J. Biol. Chem. 258, 4641-4647 (1983).
3. Ni, H. et al. Plasma fibronectin promotes thrombus growth and stability in injured arterioles. Proc. Natl. Acad. Sci. USA 100 2415-2419 (2003).
4. Yi, M., Sakai, T., Fässler, R. & Ruoslahti, E. Antiangiogenic proteins require plasma fibronectin or vitronectin for in vivo activity. Proc. Natl. Acad. Sci. USA 100, 11435-11438 (2003).
5. Nyberg, P. et al. Interactions with fibronectin attenuate the virulence of Streptococcus pyogenes. EMBO J. 23, 2166-2174 (2004).
6. Sakai, T. et al. Plasma fibronectin supports neuronal survival and reduces brain injury following transient focal cerebral ischemia but is not essential for skin-wound healing and hemostasis. Nat. Med. 7, 324-330 (2001).
7. Moretti, F.A. et al. A major fraction of fibronectin present in the extracellular matrix of tissues is plasma-derived. J. Biol. Chem. 282, 28057-28062 (2007).
8. Katagiri, Y., Brew, S.A. & Ingham, K.C. All six modules of the gelatin-binding domain of fibronectin are required for full affinity. J. Biol. Chem. 278, 11897-11902 (2003).
9. Engvall, E., Ruoslahti, E. & Miller, E.J. Affinity of fibronectin to collagens of different genetic types and to fibrinogen. J. Exp. Med. 147, 1584-1595 (1978).
10. Vuento, M., Salonen, E., Österlund, K. & Stenman, U-H. Essential charged amino acids in the binding of fibronectin to gelatin. Biochem. J. 201, 1-8 (1982).
11. Amrani, D.L. et al. Separation and analysis of the major forms of plasma fibronectins. Biochim. Biophys. Acta. 748, 308-320 (1983).
12. Mosher, D.F. Cross-linking of cold-insoluble globulin by fibrin-stabilizing factor. J. Biol. Chem. 250, 6614-6621 (1975).
13. Dessau, W., Jilek, F, Adelman, B.C. & Hormann, H. Similarity of antigelatin factor and cold insoluble globulin. Biochim. Biophys. Acta. 533, 227-237 (1978).
14. Ruoslahti, E., Hayman, E.G., Pierschbacher, M. & Engvall, E. Fibronectin: Purification, immunochemical properties and biological activities. Meth. Enzymol. 82, 803-831 (1982).
15. Yamada, K.M., Kennedy, D.W. & Hayashi, M. Fibronectin in cell adhesion, differentiation and growth. Cold Spring Harbor Conf. Cell. Prolif. 9, 131-143 (1982).
16. Miekka, S.I., Ingham, K.C. & Menache, D. Rapid methods for isolation of human plasma fibronectin Thromb. Res. 27, 1-14 (1982).
17. Vuento, M. & Vaheri, A. Purification of fibronectin from human plasma by affinity chromatography under non-denaturating conditions. Biochem. J. 183, 331-337 (1979).