Cloning, expression, and immunological characterization of the P30 protein of Mycoplasma pneumoniae

Clinical and Vaccine Immunology

Volumn 15, Issue 2, 2008, Pages 215-220

  Varshney, Avanish Kumar ^a   Chaudhry, Rama ^a   Kabra, Sushil Kumar ^a   Malhotra, Pawan ^b  

^a ALL INDIA INSTITUTE OF MEDICAL SCIENCES   (India)

^b INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY ICGEB   (India)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIGEN; FREUND ADJUVANT; HYBRID PROTEIN; MALTOSE BINDING PROTEIN P30B FUSION PROTEIN; MEMBRANE PROTEIN; PROLINE; PROTEIN P30; ADHESIN; BACTERIAL ANTIGEN; BACTERIAL DNA; DIAGNOSTIC AGENT; P30 ADHESIN, MYCOPLASMA; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL EXPERIMENT; ANTIBODY RESPONSE; ARTICLE; ASTHMA; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; CARBOXY TERMINAL SEQUENCE; CELL ADHESION; CELL ORGANELLE; CONTROLLED STUDY; CYTOPLASM; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FEMALE; GENE AMPLIFICATION; GENE SEQUENCE; GENETIC VARIABILITY; HUMAN; IMMUNOBLOTTING; IMMUNOGENICITY; INDIA; MOLECULAR CLONING; MOUSE; MYCOPLASMA PNEUMONIA; MYCOPLASMA PNEUMONIAE; NONHUMAN; NUCLEOTIDE SEQUENCE; P30 GENE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROKARYOTE; PROTEIN EXPRESSION; SENSITIVITY AND SPECIFICITY; SERODIAGNOSIS; WESTERN BLOTTING; ADOLESCENT; ANIMAL; CHEMISTRY; CHILD; COMPARATIVE STUDY; DNA SEQUENCE; GENE EXPRESSION; GENETIC POLYMORPHISM; GENETICS; IMMUNOLOGY; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; MOLECULAR GENETICS; PRESCHOOL CHILD;

ADHESINS, BACTERIAL; ADOLESCENT; ANIMALS; ANTIGENS, BACTERIAL; CHILD; CHILD, PRESCHOOL; CLONING, MOLECULAR; DNA, BACTERIAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FEMALE; GENE EXPRESSION; HUMANS; MICE; MOLECULAR SEQUENCE DATA; MYCOPLASMA PNEUMONIAE; PNEUMONIA, MYCOPLASMA; POLYMORPHISM, GENETIC; RECOMBINANT FUSION PROTEINS; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA;

EID: 40449099776     PISSN: 15566811     EISSN: 1556679X     Source Type: Journal    
DOI: 10.1128/CVI.00283-07     Document Type: Article

References (22)

1. Anonymous. 2003. British guideline on the management of asthma. Thorax 58(Suppl. 1):1-94.
2. Baseman, J. B. 1993. The cytadhesins of Mycoplasma pneumoniae and M. genitalium. Subcell. Biochem. 20:243-259.
3. Baseman, J. B., J. Morrison-Plummer, D. Drouillard, B. Puleo-Scheppke, V. V. Tryon, and S. C. Holt. 1987. Identification of a 32-kilodalton protein of Mycoplasma pneumoniae associated with hemadsorption. Isr. J. Med. Sci. 23:474-479.
4. Baseman, J. B., S. P. Reddy, and S. F. Dallo. 1996. Interplay between mycoplasma surface proteins, airway cells, and the protean manifestations of mycoplasma-mediated human infections. Am. J. Respir. Crit. Care Med. 154:S137-S144.
5. Biscardi, S., M. Lorrot, E. Marc, F. Moulin, B. Boutonnat-Faucher, C. Heilbronner, J. L. Iniguez, M. Chaussain, E. Nicand, J. Raymond, and D. Gendrel. 2004. Mycoplasma pneumoniae and asthma in children. Clin. Infect. Dis. 38:1341-1346.
6. Chaudhry, R., A. K. Varshney, and P. Malhotra. 2007. Adhesion proteins of Mycoplasma pneumoniae. Front. Biosci. 12:690-699.
7. Clyde, W. A., Jr. 1993. Clinical overview of typical Mycoplasma pneumoniae infections. Clin. Infect. Dis. 17(Suppl. 1):S32-S36.
8. Dallo, S. F., A. Chavoya, and J. B. Baseman. 1990. Characterization of the gene for a 30-kilodalton adhesion-related protein of Mycoplasma pneumoniae. Infect. Immun. 58:4163-4165.
9. Dallo, S. F., A. L. Lazzell, A. Chavoya, S. P. Reddy, and J. B. Baseman. 1996. Biofunctional domains of the Mycoplasma pneumoniae P30 adhesin. Infect. Immun. 64:2595-2601.
10. Dumke, R., I. Catrein, E. Pirkil, R. Herrmann, and E. Jacobs. 2003. Sub-typing of Mycoplasma pneumoniae isolates based on extended genome sequencing and on expression profiles. Int. J. Med. Microbiol. 292:513-525.
11. Gerstenecker, B., and E. Jacobs. 1990. Topological mapping of the P1-adhesin of Mycoplasma pneumoniae with adherence-inhibiting monoclonal antibodies. J. Gen. Microbiol. 136:471-476.
12. Hasselbring, B. M., J. L. Jordan, and D. C. Krause. 2005. Mutant analysis reveals a specific requirement for protein P30 in Mycoplasma pneumoniae gliding motility. J. Bacteriol. 187:6281-6289.
13. Hasselbring, B. M., J. L. Jordan, R. W. Krause, and D. C. Krause. 2006. Terminal organelle development in the cell wall-less bacterium Mycoplasma pneumoniae. Proc. Natl. Acad. Sci. USA 103:16478-16483.
14. Hu, P. C., A. M. Collier, and J. B. Baseman. 1977. Surface parasitism by Mycoplasma pneumoniae of respiratory epithelium. J. Exp. Med. 145:1328-1343.
15. Kannan, T. R., and J. B. Baseman. 2000. Expression of UGA-containing Mycoplasma genes in Bacillus subtilis. J. Bacteriol. 182:2664-2667.
16. Layh-Schmitt, G., H. Hilbert, and E. Pirkl. 1995. A spontaneous hemad-sorption-negative mutant of Mycoplasma pneumoniae exhibits a truncated adhesin-related 30-kilodalton protein and lacks the cytadherence-accessory protein HMW1. J. Bacteriol. 177:843-846.
17. Layh-Schmitt, G., R. Himmelreich, and U. Leibfried. 1997. The adhesin related 30-kDa protein of Mycoplasma pneumoniae exhibits size and antigen variability. FEMS Microbiol. Lett. 152:101-108.
18. Razin, S., and E. Jacobs. 1992. Mycoplasma adhesion. J. Gen. Microbiol. 138:407-422.
19. Romero-Arroyo, C. E., J. Jordan, S. J. Peacock, M. J. Willby, M. A. Farmer, and D. C. Krause. 1999. Mycoplasma pneumoniae protein P30 is required for cytadherence and associated with proper cell development. J. Bacteriol. 181:1079-1087.
20. Seggev, J. S., I. Lis, R. Siman-Tov, R. Gutman, H. Abu-Samara, G. Schey, and Y. Naot. 1986. Mycoplasma pneumoniae is a frequent cause of exacerbation of bronchial asthma in adults. Ann. Allergy 57:263-265.
21. Stauffer, G. V., M. D. Plamann, and L. T. Stauffer. 1981. Construction and expression of hybrid plasmids containing the Escherichia coli glyA genes. Gene 14:63-72.
22. Taylor-Robinson, D. 1996. Infections due to species of Mycoplasma and Ureaplasma: an update. Clin. Infect. Dis. 23:671-682.