Preparation of hydrophilic epoxy polymer magnetic beads and their immobilization for penicillin G acylase

Huagong Xuebao/Journal of Chemical Industry and Engineering (China)

Volumn 59, Issue 2, 2008, Pages 443-449

  Xue, Ping ^a   Liu, Haifeng ^a   Yang, Jinhui ^a  

^a NINGXIA UNIVERSITY   (China)

Author keywords

Catalytic activity; Hydrophilic epoxy polymer; Immobilized penicillin G acylase; Magnetic beads

Indexed keywords

CATALYST ACTIVITY; ENZYME IMMOBILIZATION; ENZYMES; HYDROPHILICITY; MAGNETIC FIELDS; POLYMERIZATION; PORE SIZE; SPECIFIC SURFACE AREA; THERMODYNAMIC PROPERTIES;

HYDROPHILIC EPOXY POLYMER; IMMOBILIZED PENICILLIN G ACYLASE; MAGNETIC BEADS;

POLYMERS;

EID: 40449086079     PISSN: 04381157     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (12)

1. Kallenberg A I, Rantwijk F, Sheldon R A. Immobilization of penicillin G acylase: The key to optimum performance. Adv. Synth. Catal., 2005, 347: 905-926
2. Xue P, Lu G Z, Guo Y I., Wang Y S, Guo Y. A novel support of MCM-48 molecular sieve for immobilization of penicillin G asylase. J. Mai. Catal., B: Enzym., 2004, 30: 75-81
3. Maria A S, Zhao X S. Design of large-pore mesoporous material for immobilization of penicillin G acylase biocatalyst. Catal. Today, 2001, 93/94/95: 293-299
4. Gao Bo, Zhu Guangshan, Fu Xueqi, Teng Lirong, Chen Jing, Wang Chunyan, Qiu Shilun. Modification of mesoporous material and stability of immobilization of penicillin G acylase. Chemical Journal of Chinese Universities, 2006. 27 (10): 1823-1826
5. Xue P, Lu G Z, Liu W Y. Poly (GMA/MA/MBAA) copolymer beads: A highly efficient support immobilizing penicillin G acylase. Chin. Chem. Lett., 2006, 17: 129-132
6. Guan Yueping, Jiang Bo, Zhu Xinghua, Liu Huizhou. Recent developments of magnetic separation in biotechnology (I): Preparation of magnetic carriers and surface chemical modification. Journal of Chemical Industry and Engineering (China). 2000, 51 (suppl.): 315-319
7. Wang Wei, Zeng Lixi, Liu Linlin, Deng Le. Preparation and application of epoxidized hydroxyl magnetic microspheres for immobilization of penicillin G acylase. Chinese Journal of Pharmaceuticals, 2005, 37: 398-401
8. Sundberg L, Porath J. Preparation of adsorbents for biospecific affinity chromatography. J. Chromalogr., 1974, 90: 87-98
9. Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 1976, 72: 248-256
10. An Lijuan, Li Zhaoqiang, Xu Wei, Chen Xinfang, Yang Bai. Preparation and characterization of superparamagnetic polymer microspheres. Chemical Journal of Chinese Universities, 2005, 26: 366-369
11. Torres R, Mateo C, Fernandez-Lorente G, Ortiz C, Fuentes M, Palomo J M, Guisan J M, Fernández-Lafuente R. A novel heterofunctional epoxy-amino sepabeads for a new enzyme immobilization protocol: Immobilization-stabilization of β-galactosidase from Aspergillus oryzae. Biotechnol. Prog., 2003, 19: 1056-1060
12. Han Hui, Xu Guanzhu. Studies on the immobilized penicillin acylase on polymer beads. Acta Microbiologica Sinica, 2001, 41: 204-208