Improved 2D nano-LC/MS for proteomics applications: A comparative analysis using yeast proteome

Journal of Biomolecular Techniques

Volumn 15, Issue 2, 2004, Pages 134-143

  Nagele E ^a   Vollmer, M ^a   Horth P ^a  

^a AGILENT TECHNOLOGIES   (Germany)

Author keywords

2D LC MS; Multidimensional LC; Online 2D LC MS; Proteomics; Yeast proteome

Indexed keywords

PEPTIDE FRAGMENT; PROTEOME; SACCHAROMYCES CEREVISIAE PROTEIN;

ARTICLE; CHEMISTRY; COMPARATIVE STUDY; ELECTROSPRAY MASS SPECTROMETRY; INSTRUMENTATION; ION EXCHANGE CHROMATOGRAPHY; ISOELECTRIC POINT; METABOLISM; METHODOLOGY; MOLECULAR WEIGHT; NANOTECHNOLOGY; PROTEIN DATABASE; PROTEOMICS; SACCHAROMYCES CEREVISIAE;

CHROMATOGRAPHY, ION EXCHANGE; DATABASES, PROTEIN; ISOELECTRIC POINT; MOLECULAR WEIGHT; NANOTECHNOLOGY; PEPTIDE FRAGMENTS; PROTEOME; PROTEOMICS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 4043171443     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: None     Document Type: Article

References (7)

1. Davis MT, Beierle J, Bures ET, McGinley, et al. Automated LC-LC-MS-MS platform using binary ionexchange and gradient reversed-phase chromatography for improved proteomic analyses. J Chromatogr B 2001;752:281-291.
2. Nägele E, Vollmer M, Hörth P. Two-dimensional nanoliquid chromatography-mass spectrometry system for applications in proteomics. J Chromatogr A 2003;1009:197-205.
3. Washburn MP, Wolters D, Yates III JR. Large-scale analysis of the yeast proteome by multidimensional protein identification technology. Nat Biotechnol 2001;19:242-247.
4. Florens L, Washburn MP, Raine JD, et al. A proteomic view of the Plasmodium falciparum life cycle. Nature 2002;419:520-526.
5. Vollmer M, Nägele E, Hörth P. Differential proteome analysis: Two-dimensional nano LC/MS of E. coli proteome grown on different carbon sources. J Biomol Techn 2003; 14: 128-135.
6. Hörth P, Nägele E, Vollmer M. Proteome profiling of E. coli: Effect of heat-shock conditions on protein expression pattern. LC/GC Europe 2003;16:641-647.
7. Moritz R., Vollmer M., Nägele E. On-line and off-line 2D LC-ESI-MS/MS methods in proteomic analysis. Pharmagenomics 2004; accepted for publication.