Pituitary synaptic protein SNAP-25 sensitive to GnRH is necessary for LH release

Endocrine Regulations

Volumn 38, Issue 1, 2004, Pages 1-6

  Quintanar, J Luis ^a,b   Salinas, Eva ^a   Chávez Morales, Rosa María ^a   Quintanar Stephano, Andrés ^a  

^a UNIVERSIDAD AUTÓNOMA DE AGUASCALIENTES   (Mexico)

^b Autonomous University of Aguascalientes Centro de Ciencias Basicas   (Mexico)

Author keywords

Digitonin; Gonadotrophs; Immunoblot; Immunohistochemistry; Permeabilized cells

Indexed keywords

DIGITONIN; GONADORELIN; LUTEINIZING HORMONE; SYNAPTOSOMAL ASSOCIATED PROTEIN 25;

ADENOHYPOPHYSIS; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; CONTROLLED STUDY; EXOCYTOSIS; IMMUNOBLOTTING; IMMUNOCHEMISTRY; IN VIVO STUDY; LUTEINIZING HORMONE RELEASE; MALE; NONHUMAN; ORCHIECTOMY; RAT;

ANIMALS; CELLS, CULTURED; GENE EXPRESSION REGULATION; GONADOTROPIN-RELEASING HORMONE; IMMUNOHISTOCHEMISTRY; LUTEINIZING HORMONE; MALE; MEMBRANE PROTEINS; NERVE TISSUE PROTEINS; PITUITARY GLAND, ANTERIOR; RATS; RATS, WISTAR; SYNAPTOSOMAL-ASSOCIATED PROTEIN 25;

EID: 4043171347     PISSN: 12100668     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. AGUADO F, GOMBAU L, MAJÓ G, MARSAL J, BLANCO J, BLASI J: Regulated secretion is impaired in AtT-20 endocrine cells stably transfected with botulinum neurotoxin type A light chain. J Biol Chem 272, 26005-26008, 1997
2. AGUADO F, MAJÓ G, RUIZ-MONTASELL B, CANALS JP, CASANOVA A, MARSAL J, BLASI J: Expression of synaptosomal-associated protein SNAP-25 in endocrine anterior pituitary cells. Euro J Cell Biol 69, 351-359, 1996
3. COORSSEN JR, BLANK PS, ALBERTORIO F, BEZRUKOV L, KOLOSOVA I, CHEN X, BACKLUND PS, ZIMMERBERG J: Regulated secretion: SNARE density, vesicle fusion and calcium dependence. J Cell Sci 116, 2087-2097, 2003
4. GERST J: SNAREs and SNARE regulators in membrane fusion and exocytosis. Cell Mol Life Sci 55, 707-734, 1999
5. GUTIERREZ LM, QUINTANAR JL, RUEDA J, VINIEGRA S, REIG JA: The protein phosphatase inhibitor calyculin-A affects catecholamine secretion and granular distribution in cultured adrenomedullary chromaffin cells. Eur J Cell Biol 68, 88-95, 1995
6. HEPP R, LANGLEY K: SNAREs during development. Cell Tissue Res 305, 247-253, 2001
7. JACOBSSON G, MEISTER B: Molecular components of the exocytosis machinery in the rat pituitary gland. Endocrinology 137, 5344-5356, 1996
8. JACOBSSON G, RAZANI H, OGREN SO, MEISTER B: Estrogen down-regulates mRNA encoding the exocytotic protein SNAP-25 in the rat pituitary gland. J Neuroendocrinol 10, 157-163, 1998
9. LAEMMLI UK: Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227, 680-685, 1970
10. MAJO G, FERRER I, MARSAL J, BLASI J, AGUADO F: Immunocytochemical analysis of the synaptic proteins SNAP-25 and Rab3A in human pituitary adenomas. Overexpression of SNAP-25 in the mammosomatotroph lineages. J Pathol 183, 440-446, 1997
11. MAJO G, LORENZO MJ, BLASI J, AGUADO F: Exocytotic protein components in rat pituitary gland after long-term estrogen administration. J Endocrinol 161, 323-331, 1999
12. MASUMOTO N, IKEBUCHI Y, MATSUOKA T, TASAKA K, MIYAKE A, MURATA Y: Involvement of SNAP-25 in TRH-induced exocytosis in pituitary GH4C1 cells. J Endocrinol 153, R5-R10, 1997
13. MILLAR RP: GnRH II and type GnRH receptors Trends Endocrinol Metabol 14, 35-43, 2003
14. QUINTANAR JL, SALINAS E: effect of hypothyroidism on synaptosomal- associated protein of 25 kda and syntaxin-1 expression in adenohypophyses of rat. J Endocrinol Invest 25, 754-758, 2002
15. SALINAS E, QUINTANAR JL, REIG JA: Immunohistochemical study of syntaxin-1 and SNAP-25 in the pituitaries of mouse, guinea pig and cat. Acta Physiol Pharmacol Therapeut Latinoamerican 49, 61-64, 1999
16. SIGNORELLA A, HYMER WC: An enzyme-linked immunosorbent assay for rat prolactin. Anal Biochem 136, 372-381, 1984
17. STEFANEANU L, KOVACS K, HORVATH E, LOSINSKI EN, MAYERHOFER A, WAGNER TE, BARTKE A: An immunocytochemical and ultrastructural study of adenohypophyses of mice transgenic for human growth hormone. Endocrinology 126, 608-615, 1990
18. STOJILKOVIC SS, REINHART J, CATT, KJ: Gonadotropin-releasing hormone receptors: Structure and signal transdution pathways. Endocrine Rev 15, 462-499, 1994
19. TOWBIN H, STAEHELIN J, GORDON J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Proc Nat Acad Sci USA 76, 4350-4354, 1979
20. WYNICK D, BLOOM S: Magnetic bead separation of anterior pituitary cells. Neuroendocrinology 52, 560-565, 1990