An equation for calculating the volumetric ratios required in a ligation reaction

Applied Microbiology and Biotechnology

Volumn 65, Issue 2, 2004, Pages 200-202

  Cranenburgh, R M ^a  

^a Stephenson Building   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; PLASMID DNA;

MICROBIOLOGY;

ARTICLE; CALCULATION; DNA LIGATION; DNA VECTOR; GENETIC PROCEDURES; NONHUMAN; REACTION ANALYSIS; STOICHIOMETRY;

EID: 4043160101     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-004-1577-7     Document Type: Article

References (7)

1. Bercovich JA, Grinstein S (1992) Effect of DNA concentration on recombinant plasmid recovery after blunt-end ligation. Biotechniques 12:190-193
2. Damak S, Bullock DW (1993) A simple two-step method for efficient blunt-end ligation of DNA fragments. Biotechniques 15:448-452
3. Doyle K, Miles J (1996) Ligation of plasmid vector and insert DNA. In: Promega protocols and applications guide. Promega, Madison, Wis., p 45
4. Dugaiczyk A, Boyer HW, Goodman HM (1975) Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structures. J Mol Biol 96:171-184
5. Hanahan D, Jessee J, Bloom FR (1991) Plasmid transformation of Escherichia coli and other bacteria. Methods Enzymol 204:63-113
6. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: A laboratory manual. Cold Spring Harbor, New York, pp 1.63-1.73
7. Seidman CE, Struhl K, Sheen J (1994) Introduction of plasmid DNA into cells. In: Ausubel FM, Brent R, Kingston ROE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) Current protocols in molecular biology. Wiley, New York, pp 181-188