Expression and identification of mutated osteoprotegerin in culture cells and larvae of silkworm

Acta Biochimica et Biophysica Sinica

Volumn 36, Issue 5, 2004, Pages 331-335

  Xiao, Hai Long ^a   Jin, Yong Feng ^a   Zhang, Yao Zhou ^b  

^a ZHEJIANG UNIVERSITY   (China)

^b ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Hypocalcemic effect; OPG 372; Silkworm baculovirus expression system

Indexed keywords

OSTEOPROTEGERIN; RECOMBINANT PROTEIN; VIRUS DNA; CALCIUM; CELL RECEPTOR; GLYCOPROTEIN; TNFRSF11B PROTEIN, HUMAN; TNFRSF11B PROTEIN, MOUSE; TUMOR NECROSIS FACTOR RECEPTOR;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; CELL CULTURE; CONTROLLED STUDY; DRUG ACTIVITY; DRUG EFFECT; GENE INSERTION; GENE MUTATION; GENETIC TRANSFECTION; HUMAN; HYPOCALCEMIA; IMMUNOREACTIVITY; LARVA; MALE; MOUSE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN EXPRESSION; RECOMBINANT PLASMID; SEQUENCE HOMOLOGY; SILKWORM; SOUTHERN BLOTTING; VIRUS RECOMBINANT; WESTERN BLOTTING; ANIMAL; BACULOVIRUS; BLOOD; CHEMICALLY INDUCED DISORDER; DOSE RESPONSE; GENETICS; INSTITUTE FOR CANCER RESEARCH MOUSE; METABOLISM; METHODOLOGY; MUTATION; SITE DIRECTED MUTAGENESIS; VIROLOGY;

ANIMALIA; BOMBYX MORI; BOMBYX MORI NUCLEOPOLYHEDROVIRUS; DNA VIRUSES; INSERTION SEQUENCES; UNIDENTIFIED BACULOVIRUS;

ANIMALS; BACULOVIRIDAE; BOMBYX; CALCIUM; CELLS, CULTURED; DOSE-RESPONSE RELATIONSHIP, DRUG; GLYCOPROTEINS; HUMANS; HYPOCALCEMIA; LARVA; MALE; MICE; MICE, INBRED ICR; MUTAGENESIS, SITE-DIRECTED; MUTATION; OSTEOPROTEGERIN; RECEPTORS, CYTOPLASMIC AND NUCLEAR; RECEPTORS, TUMOR NECROSIS FACTOR; RECOMBINANT PROTEINS; TRANSFECTION;

EID: 4043107106     PISSN: 05829879     EISSN: None     Source Type: Journal    
DOI: 10.1093/abbs/36.5.331     Document Type: Article

References (13)

1. Simonet WS, Lacey DL, Dunstan CR, Kelley M, Chang MS, Lüthy R, Nguyen HQ et al. Osteoprotegerin: A novel secreted protein involved in the regulation of bone density. Cell, 1997, 89: 309-319
2. Yamamoto M, Murakami T, Nishikawa M, Tsuda E, Mochizuki SI, Higashio K, Akatsu T et al. Hypocalcemic effect of osteoclastogenesis inhibitory factor/osteoprotegerin in the thyroparathyroidectomized rat. Endocrinology, 1998, 139(9): 4012-4015
3. He ZY, Yang GZ, Zhang WJ, Wu XF. Cloning and expression of a novel mutated osteoprotegerin/osteoclastogenesis inhibitory factor gene. Acta Biochim Biophys Sin, 1999, 31(6): 680-684
4. He ZY, Yang GZ, Chen ZY, Li B, Zhang WJ, Wu XF. A novel isoform of osteoprotegerin gene: Cloning and expression and its hypocalcemic effect in mice. Protein and Peptide Letters, 2000, 7(4): 233-240
5. Sambrook J, Fritsch EF, and Maniatis T eds. Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor laboratory Press, 1989
6. Qian DM, Shen GQ, Ke RL. Biological assay of calcitonin by blood calcium determination in rats. Chinese Journal of Pharmaceutical Analysis, 1994, 14 (3): 30-34
7. Lü HS. Molecular Biology of Insect Viruses. Beijing: China Agricultural Scientech Press, 1998, 547-580
8. Tsuda E, Goto M, Mochizuki S, Yano K, Kobayashi F, Morinaga T, Higashio K. Isolation of a novel cytokine from human fibroblasts that specifically inhibits osteoclastogenesis. Biochem Biophys Res Commun, 1997, 234: 137-142
9. Kong YY, Penninger JM. Molecular control of bone remodeling and osteoporosis. Exp Gerontol, 2000, 35: 947-956
10. Yamaguchi K, Kinosaki M, Goto M, Kobayashi F, Tsuda E, Morinaga T, Higashio K. Characterization of structural domains of human osteoclastogenesis inhibitory factor. J Biol Chem, 1998, 273(9): 5117-5123
11. Yasuda H, Shima N, Nakagawa N, Mochizuki SI, Yano K, Fujise N, Sato Y et al. Identity of osteoclastogenesis inhibitory factor (OCIF) and osteoprotegerin (OPG): A mechanism by which OPG/OCIF inhibits osteoclastogenesis in vitro. Endocrinology, 1998, 139(3): 1329-1337
12. Suda T, Takahashi N, Udagawa N, Jimi E, Gillespie MT, Martin TJ. Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families. Endocr Rev, 1999, 20 (3): 345-357
13. Akatsu T, Murakami T, Ono K, Nishikawa M, Tsuda E, Mochizuki SI, Fujise N et al. Osteoclastogenesis inhibitory factor exhibits hypocalcemic effects in normal mice and in hypercalcemic nude mice carrying tumors associated with humoral hypercalcemia of malignancy. Bone, 1998, 23(6): 495-498