Fabrication of macroporous hydrogel membranes using photolithography for enzyme immobilization

Journal of Chemical Technology and Biotechnology

Volumn 83, Issue 3, 2008, Pages 252-259

  Choi, Dongkil ^a   Lee, Woojin ^a   Lee, Yeol ^a   Kim, Dae Nyun ^a   Park, Jinwon ^a   Koh, Won Gun ^a  

^a Yonsei University   (South Korea)

Author keywords

Enzyme immobilization; Macropores; Photolithography; Poly(ethylene glycol) hydrogel

Indexed keywords

CROSSLINKING; DENSITY (SPECIFIC GRAVITY); ENZYME IMMOBILIZATION; HYDROGELS; MOLECULAR WEIGHT; PHOTOLITHOGRAPHY; PHOTOMASKS; PHOTOPOLYMERIZATION;

PORE DENSITY; VOLUME RATIO;

POLYETHYLENE GLYCOLS;

GLUCOSE OXIDASE; MACROGOL;

CROSSLINKING; DENSITY (SPECIFIC GRAVITY); ENZYME IMMOBILIZATION; HYDROGELS; MOLECULAR WEIGHT; PHOTOLITHOGRAPHY; PHOTOMASKS; PHOTOPOLYMERIZATION; POLYETHYLENE GLYCOLS;

ARTICLE; BIOTECHNOLOGY; CHEMICAL STRUCTURE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME IMMOBILIZATION; ENZYME INACTIVATION; ENZYME MECHANISM; GLUCOSE OXIDATION; MEMBRANE PERMEABILITY; MEMBRANE TECHNOLOGY; POLYMERIZATION; POROSITY;

EID: 40149108909     PISSN: 02682575     EISSN: 10974660     Source Type: Journal    
DOI: 10.1002/jctb.1800     Document Type: Article

References (30)

1. Bornscheuer UT, Immobilizing enzymes: how to create more suitable biocatalysts Ang Chem-Int Edn 42:3336-3337 (2003).
2. Monsan P and Combes D, Enzyme stabilization by immobilization. Methods Enzymol 137:584-598 (1988).
3. Franchina JG, Lackowski WM, Dermody DL, Crooks RM, Bergbreiter DE, Sirkar K et al., Electrostatic immobilization of glucose oxidase in a weak acid, polyelectrolyte hyperbranched ultrathin film on gold: fabrication, characterization, and enzymatic activity. Anal Chem 71:3133-3139 (1999).
4. Brahim S, Narinesingh D and Guiseppi-Elie A, Kinetics of glucose oxidase immobilized in p(HEMA)-hydrogel microspheres in a packed-bed bioreactor. J Mol Catal B-Enzymatic 18:69-80 (2002).
5. Pekel N, Salih B and Guven O, Enhancement of stability of glucose oxidase by immobilization onto metal ion-chelated poly(N-vinyl imidazole) hydrogels. J Biomater Sci-Polym Edn 16:253-266 (2005).
6. Kunzelmann U and Bottcher H, Biosensor properties of glucose oxidase immobilized within SiO2 gels. Sensors Actuators B 39:222-228 (1997).
7. Sawhney AS, Pathak CP and Hubbell JA, Bioerodible hydrogels based on photopolymerized poly(ethylene glycol)-co-poly(alpha-hydroxy acid) diacrylate macromers. Macromolecules 26:581-587 (1993).
8. Sawhney AS, Pathak CP, van Rensburg JJ, Dunn RC and Hubbell JA, Optimization of photopolymerized bioerodible hydrogel properties for adhesion prevention. J Biomed Mater Res 28:831-838 (1994).
9. Quinn CP, Pishko MV, Hubbell JA and Heller A, Kinetics of glucose delivery to subcutaneous tissue in rats measured with 0.3 mm amperometric microsensors. Am J Physiol 269:E155-E161 (1995).
10. Pathak CP, Sawhney AS and Hubbell JA, Rapid photopolymerization of immunoprotective gels in contact with cells and tissue. J Am Chem Soc 114:8311-8312 (1992).
11. Mann BK, Gobin AS, Tsai AT, Schmedlen RH and West JL, Smooth muscle cell growth in photopolymerized hydrogels with cell adhesive and proteolytically degradable domains: synthetic ECM analogs for tissue engineering. Biomaterials 22:3045-3051 (2001).
12. Andreopoulos FM, Roberts MJ, Bentley MD, Harris JM, Beckman EJ and Russell AJ, Photoimmobilization of organophosphorus hydrolase within a PEG-based hydrogel. Biotechnol Bioeng 65:579-588 (1999).
13. Belgoudi J and Fortier G, Poly(ethylene glycol)-bovine serum albumin hydrogel as a matrix for enzyme immobilization. In vitro biochemical characterization. J Bioactive Compatible Polym 14:31-53 (1999).
14. Russell RJ, Pishko MV, Simonian AL and Wild JR, Poly (ethylene glycol) hydrogel-encapsulated fluorophore-enzyme conjugates for direct detection of organophosphorus neurotoxins. Anal Chem 71:4909-4912 (1999).
15. Rounds RM, Ibey BL, Beier HT, Pishko MV and Cote GL, Microporated PEG spheres for fluorescent analyte detection. Journal of Fluorescence 17:57-63 (2007).
16. Chen J, Park H and Park K, Synthesis of superporous hydrogels: hydrogels with fast swelling and superabsorbent properties. J Biomed Mater Res 44:53-62 (1999).
17. Ko H and Kameoka J, Photo-crosslinked porous PEG hydrogel membrane via electrospinning. J Photopolym Sci Technol 19:413-418 (2006).
18. Quinn CP, Pathak CP, Heller A and Hubbell JA, Photo-crosslinked copolymers of 2-hydroxyethyl methacrylate, poly(ethylene glycol) tetra-acrylate and ethylene dimethacrylate for improving biocompatibility of biosensors. Biomaterials 16:389-396 (1995).
19. Hern DL and Hubbell JA, Incorporation of adhesion peptides into nonadhesive hydrogels useful for tissue resurfacing. J Biomed Mater Res 39:266-276 (1998).
20. Brinkley M, A brief survey of methods for preparing protein conjugates with dyes, haptens, and cross-linking reagents. Bioconjugate Chem 3:2-13 (1992).
21. Zhu HG, Srivastava R, Brown JQ and McShane MJ, Combined physical and chemical immobilization of glucose oxidase in alginate microspheres improves stability of encapsulation and activity. Bioconjugate Chem 16:1451-1458 (2005).
22. Mellott MB, Searcy K and Pishko MV, Release of Protein from highly cross-linked hydrogels of poly(ethylene glycol) diacrylate fabricated by UV polymerization. Biomaterials 22:929-941 (2001).
23. Haraldsson KT, Hutchison JB, Sebra RP, Good BT, Anseth KS and Bowman CN, 3D polymeric microfluidic device fabrication via contact liquid photolithographic polymerization (CLiPP). Sensors Actuators B 113:454-460 (2006).
24. Revzin A, Russell RJ, Yadavalli VK, Koh W, Deister C, Hile DD et al., Fabrication of poly(ethylene glycol) hydrogel microstructures using photolithography. Langmuir 17:5440-5447 (2001).
25. Ghandhi SK, VLSI Fabrication Principles. John Wiley & Sons, New York (1994).
26. Reiser A, Photoreactive Polymers. The Science and Technology of Resists. John Wiley & Sons, New York (1989).
27. Sirkar K and Pishko MV, Amperometric biosensors based on oxidoreductases immobilized in photopolymerized poly(ethylene glycol) redox polymer hydrogels. Anal Chem 70:2888-2894 (1998).
28. Cassagneau T and Caruso F, Conjugated polymer inverse opals for potentiometric biosensing. Adv Mater 14:1837-1841 (2002).
29. Peppas NA and Bar-Howel BD, Hydrogels in Medicine and Pharmacy. CRC Press, Boca Raton, FL, pp 27-56 (1986).
30. Cruise GM, Scharp DS and Hubbell JA, Characterization of permeability and network structure of interfacially photopolymerized poly(ethylene glycol) diacrylate hydrogels. Biomaterials 19:1287-1294 (1998).