Liposome encapsulation of conjugated polyelectrolytes: Toward a liposome beacon

Journal of the American Chemical Society

Volumn 130, Issue 2, 2008, Pages 457-459

  An, Thien Ngo ^a   Karam, Pierre ^a   Fuller, Elisa ^a   Burger, Melanie ^a   Cosa, Gonzalo ^a  

^a MCGILL UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

1,2 DIOLEOYL 3 TRIMETHYLAMMONIOPROPANE; ELECTROLYTE; LIPOSOME; PHENYL GROUP; 3,3' DIOCTADECYLINDOCARBOCYANINE; 3,3'-DIOCTADECYLINDOCARBOCYANINE; CARBOCYANINE; FLUORESCENT DYE; POLY(2,5 METHOXY PROPYLOXY SULFONATE PHENYLENE VINYLENE); POLY(2,5-METHOXY-PROPYLOXY SULFONATE PHENYLENE VINYLENE); POLYVINYL DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; CONJUGATION; ENCAPSULATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; HYDRODYNAMICS; LIGHT SCATTERING; LIPOPHILICITY; METHODOLOGY; STRUCTURE ANALYSIS; CHEMISTRY; FLUORESCENCE;

CARBOCYANINES; ELECTROLYTES; FLUORESCENCE; FLUORESCENT DYES; LIPOSOMES; POLYVINYLS;

EID: 40149107623     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja076217b     Document Type: Article

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26. Briefly, immobilization involved (a) the functionalization of glass coverslips with Vectabond reagent, (b) the covalent tethering of a 100/1 PEG (MW 5000)/biotin-PEG (MW 5000) mixture to the coverslip surface, (c) the assembly of a flow chamber, (d) a 10 min incubation of a streptavidin solution and (e) a 3-4 min incubation of the 5 pM liposome solution under study followed by a steady flow of buffer solution to remove all non-immobilized liposomes and non-encapsulated MPS-PPV.