Purification of His6-tagged Photosystem I from Chlamydomonas reinhardtii

Photosynthesis Research

Volumn 96, Issue 1, 2008, Pages 51-60

  Gulis, Galina ^a   Narasimhulu, Kuppala V ^a   Fox, Lisa N ^a   Redding, Kevin E ^a,b  

^a UNIVERSITY OF ALABAMA   (United States)

^b INST DE BIOLOGIE PHYSICO CHIMIQUE   (France)

Author keywords

Chlamydomonas reinhardtii; Epitope tagging; His tag; Membrane protein; Photosystem 1; Reaction center

Indexed keywords

CHLAMYDOMONAS REINHARDTII;

ALGAL PROTEIN; HIS HIS HIS HIS HIS HIS; HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; OLIGOPEPTIDE;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CHEMISTRY; CHLAMYDOMONAS REINHARDTII; IMMUNOBLOTTING; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; PHOTOSYSTEM I; SEQUENCE HOMOLOGY; THYLAKOID;

ALGAL PROTEINS; AMINO ACID SEQUENCE; ANIMALS; CHLAMYDOMONAS REINHARDTII; HISTIDINE; IMMUNOBLOTTING; MOLECULAR SEQUENCE DATA; OLIGOPEPTIDES; PHOTOSYSTEM I PROTEIN COMPLEX; SEQUENCE HOMOLOGY, AMINO ACID; THYLAKOIDS;

EID: 39849111322     PISSN: 01668595     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11120-007-9283-9     Document Type: Article

References (36)

1. Amunts A, Drory O, Nelson N (2007) The structure of a plant photosystem I supercomplex at 3.4 Å resolution. Nature 447:58-63
2. Ben-Shem A, Frolow F, Nelson N (2003) Crystal structure of plant photosystem I. Nature 426:630-635
3. Boudreaux B, MacMillan F, Teutloff C et al (2001) Mutations in both sides of the photosystem I reaction center identify the phylloquinone observed by electron paramagnetic resonance spectroscopy. J Biol Chem 276:37299-37306
4. Fischer N, Stampacchia O, Redding K et al (1996) Selectable marker recycling in the chloroplast. Mol Gen Genet 251:373-380
5. Fischer N, Setif P, Rochaix JD (1997) Targeted mutations in the psaC gene of Chlamydomonas reinhardtii: preferential reduction of FB at low temperature is not accompanied by altered electron flow from photosystem I to ferredoxin. Biochem 36:93-102
6. Fromme P, Bottin H, Krauss N et al (2002) Crystallization and electron paramagnetic resonance characterization of the complex of photosystem I with its natural electron acceptor ferredoxin. Biophys J 83:1760-1773
7. Golbeck JH, Bryant DA (1991) Photosystem I. In: Lee CP (ed) Current topics in bioenergetics: light driven reactions in bioenergetics. Academic Press, New York
8. Goldschmidt-Clermont M (1991) Transgenic expression of aminoglycoside adenine transferase in the chloroplast: a selectable marker for site-directed transformation of Chlamydomonas. Nucl Acids Res 19:4083-4089
9. Goldschmidt-Clermont M, Girard-Bascou J, Choquet Y et al (1990) Trans-splicing mutants of Chlamydomonas reinhardtii. Mol Gen Genet 223:417-425
10. Goldsmith J, Boxer S (1996) Rapid isolation of bacterial photosynthetic reaction centers with an engineered poly-histidine tag. Biochim Biophys Acta 1276:171-175
11. Goldsmith JO, King B, Boxer SG (1996) Mg coordination by amino acid side chains is not required for assembly and function of the special pair in bacterial photosynthetic reaction centers. Biochem 35:2421-2428
12. Harris EH (1989) The Chlamydomonas sourcebook. A comprehensive guide to biology and laboratory use. Academic Press, San Diego
13. Henderson JN, Zhang J, Evans BW et al (2003) Disassembly and degradation of photosystem I in an in vitro system are multievent, metal-dependent processes. J Biol Chem 278:39978-39986
14. 6 and plastocyanin to photosystem I of Chlamydomonas reinhardtii requires PsaF. Biochem 36:6343-6349
15. Joliot P, Béal D, Frilley B (1980) Une nouvelle méthode spectrophotométrique destinée à l'étude des réactions photosynthétiques. Journal de chimie physique 77:209-216
16. Jordan P, Fromme P, Witt HT et al (2001) Three-dimensional structure of cyanobacterial photosystem I at 2.5 A resolution. Nature 411:909-917
17. B in photosystem I. Proposed chemical rescue of a [4Fe-4S] cluster with an external thiolate in alanine, glycine, and serine mutants of PsaC. J Biol Chem 271:31135-31144
18. Kargul J, Nield J, Barber J (2003) Three-dimensional reconstruction of a light-harvesting complex I-photosystem I (LHCI-PSI) supercomplex from the green alga Chlamydomonas reinhardtii. Insights into light harvesting for PSI. J Biol Chem 278:16135-16141
19. Krabben L, Schlodder E, Jordan R et al (2000) Influence of the axial ligands on the spectral properties of P700 of photosystem I: a study of site-directed mutants. Biochem 39:13012-13025
20. Kück U, Choquet Y, Schneider M et al (1987) Structural and transcriptional analysis of two homologous genes for the P700 chlorophyll a-apoproteins in Chlamydomonas reinhardtii: evidence for in vivo trans-splicing. EMBO J 6:2185-2192
21. +. Biochem 38:13210-13215
22. Müller MG, Niklas J, Lubitz W et al (2003) Ultrafast transient absoprtion studies on photosystem I reaction centers from Chlamydomonas reinhardtii I. A new interpretation of the energy trapping and early electron transfer steps in photosystem I. Biophys J 85:3899-3922
23. Murakami A (1997) Quantitative analysis of 77K fluorescence emission spectra in Synechocystis sp. PCC 6714 and Chlamydomonas reinhardtii with variable PS I/PS II stoichiometries. Photosyn Res 53:141-148
24. Perron K, Goldschmidt-Clermont M, Rochaix JD (2004) A multiprotein complex involved in chloroplast group II intron splicing. RNA 10:704-711
25. Porra R, Thompson W, Kriedemann P (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim Biophys Acta 975:384-394
26. Ramesh VM, Webber AN (2004) Rapid isolation and purification of photosystem I chlorophyll-binding protein from Chlamydomonas reinhardtii. Methods Mol Biol 274:19-28
27. Redding K, MacMillan F, Leibl W et al (1998) A systematic survey of conserved histidines in the core subunits of Photosystem I by site-directed mutagenesis reveals the likely axial ligands of P700. EMBO J 17:50-60
28. Redding K, Cournac L, Vassiliev IR et al (1999) Photosystem I is indispensable for photoautotrophic growth, CO2 fixation, and H2 photoproduction in Chlamydomonas reinhardtii. J Biol Chem 274:10466-10473
29. Rochaix JD (1978) Restriction endonuclease map of the chloroplast DNA of Chlamydomonas reinhardtii. J Mol Biol 126:597-617
30. x cluster in the Photosystem I reaction center. Protein Sci 6:340-346
31. Smith P, Krohn R, Hermanson G, Mallia A, Gartner F, Provenzano M, Fujimoto E, Goeke N, Olson B, Klenk D (1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150:76-85
32. 2+ affinity column chromatography. FEBS Lett 426:140-144
33. Takahashi Y, Goldschmidt-Clermont M, Soen S et al (1991) Directed chloroplast transformation in Chlamydomonas reinhardtii: insertional inactivation of the psaC gene encoding the iron sulfur protein destabilizes photosystem I. Embo J 10:2033-2040
34. Tang H, Chitnis PR (2000) Addition of C-terminal histidyl tags to PsaL and PsaK1 proteins of cyanobacterial photosystem I. Indian J Biochem Biophys 37:433-440
35. Vassiliev IR, Antonkine ML, Golbeck JH (2001) Iron-sulfur clusters in type I reaction centers. Biochim Biophys Acta-Bioenerg 1507:139-160
36. Witt H, Bordignon E, Carbonera D et al (2003) Species-specific differences of the spectroscopic properties of P700: analysis of the influence of non-conserved amino acid residues by site-directed mutagenesis of photosystem I from Chlamydomonas reinhardtii. J Biol Chem 278:46760-46771