A novel high-throughput format assay for HIV-1 integrase strand transfer reaction using magnetic beads

Acta Pharmacologica Sinica

Volumn 29, Issue 3, 2008, Pages 397-404

  He, Hong Qiu ^a   Ma, Xiao Hui ^a,b   Liu, Bin ^a   Chen, Wei Zu ^a   Wang, Cun Xin ^a   Cheng, Shao Hui ^a  

^a BEIJING UNIVERSITY OF TECHNOLOGY   (China)

^b UNIVERSITY OF GEORGIA   (United States)

Author keywords

High throughput; HIV 1; Integrase; Magnetic beads; Strand transfer

Indexed keywords

ANTIVIRUS AGENT; BIOTIN; DIGOXIN; DOUBLE STRANDED DNA; INTEGRASE; STREPTAVIDIN;

ARTICLE; DRUG IDENTIFICATION; HIGH THROUGHPUT SCREENING; HUMAN IMMUNODEFICIENCY VIRUS 1; IN VITRO STUDY; LONG TERMINAL REPEAT; MAGNETISM; POLYACRYLAMIDE GEL ELECTROPHORESIS;

EID: 39849087244     PISSN: 16714083     EISSN: 17457254     Source Type: Journal    
DOI: 10.1111/j.1745-7254.2008.00748.x     Document Type: Article

References (30)

1. 1 Craigie R. HIV integrase, a brief overview from chemistry to therapeutics. J Biol Chem 2001 ; 276: 213–16.
2. 2 Chiu TK, Davies DR. Structure and function of HIV‐1 integrase. Curr Top Med Chem 2004 ; 4: 965–77.
3. 3 Pluymers W, De Clercq E, Debyser Z. HIV‐1 integration as a target for antiretroviral therapy: a review. Curr Drug Targets Infect Disord 2001 ; 1: 133–49.
4. 4 Marchand C, Neamati N, Pommier Y. In vitro human immunodeficiency virus type 1 integrase assays. Methods Enzymol 2001 ; 340: 624–33.
5. 5 Chow SA. In vitro assays for activities of retroviral integrase. Methods 1997 ; 12: 306–17.
6. 6 Hazuda DJ, Hastings JC, Wolfe AL, Emini EA. A novel assay for the DNA strand‐transfer reaction of HIV‐1 integrase. Nucleic Acids Res 1994 ; 22: 1121–22.
7. 7 Hwang Y, Rhodes D, Bushman F. Rapid microtiter assays for provirus topoisomerase, mammalian type IB topoisomerase and HIV‐1 integrase: application to inhibitor isolation. Nucleic Acids Res 2000 ; 28: 4884–92.
8. 8 John S, Fletcher TM III, Jonsson CB. Development and application of a high‐throughput screening assay for HIV‐1 integrase enzyme activities. J Biomol Screen 2005 ; 10: 606–14.
9. 9 David CA, Middleton T, Montgomery D, Lim HB, Kati W, Molla A, et al. Microarray compounds screening (microARCS) to identify inhibitors of HIV integrase. J Biomol Screen 2002 ; 7: 259–66.
10. 10 Wang Y, Klock H, Yin H, Wolff K, Bieza K, Niswonger K, et al. Homogeneous high‐throughput screening assays for HIV‐1 integrase 3′‐processing and strand transfer activities. J Biomol Screen 2005 ; 10: 456–62.
11. 11 Hazuda DJ, Felock PJ, Witmer M, Wolfe A, Stillmock K, Grobler JA, et al. Inhibitors of strand transfer that prevent integration and inhibit HIV‐1 replication in cells. Science 2000 ; 287: 646–50.
12. 12 Farnet CM, Wang B, Lipford JR, Bushman FD. Differential inhibition of HIV‐1 preintegration complexes and purified integrase by small molecules. Proc Natl Acad Sci USA 1996 ; 93: 9742–7.
13. 13 He HQ, Ma XH, Liu B, Zhang XY, Chen WZ, Wang CX, et al. High‐throughput real‐time assay based on molecular beacons for HIV‐1 integrase 3′‐processing reaction. Acta Pharmacol Sin 2007 ; 28: 811–7.
14. 14 Smolov M, Gottikh M, Tashlitskii V, Korolev S, Demidyuk L. Kinetic study of the HIV‐1 DNA 3′‐end processing single‐turnover property of integrase. FEBS J 2006 ; 273: 1137–51.
15. 15 Zhang JH, Chung TD, Oldenburg KR. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 1999 ; 4: 67–73.
16. 16 Leh H, Brodin P, Bischerour J, Deprez E, Tauc P, Brochoon JC, et al. Determinants of Mg 2+ ‐dependent activities of recombinant human immunodeficiency virus type 1 integrase. Biochemistry 2000 ; 39: 9285–94.
17. 17 Hindmarsh P, Lei J. Retroviral DNA integration. Microbiol Mol Biol Rev 1999 ; 63: 836–43.
18. 18 Sherman MP, Greene WC. Slipping through the door: HIV entry into the nucleus. Microbes Infect 2002 ; 4: 67–73.
19. 19 Andrake MD, Skalka AM. Retroviral integrase, putting the pieces together. J Biol Chem 1996 ; 271: 19 633–6.
20. 20 Tramontano E, Onidi L, Esposito F, Badas R, Colla PL. The use of a new in vitro reaction substrate reproducing both U3 and U5 regions of the HIV‐1 3′‐ends increases the correlation between the in vitro and in vivo effects of the HIV‐1 integrase inhibitors. Biochem Phar 2004 ; 67: 1751–61.
21. 21 Debyser Z, Cherepanov P, Pluymers W, De Clercq E. Assays for the evaluation of HIV‐1 integrase inhibitors. Methods Mol Biol 2001 ; 160: 139–55.
22. 22 Lee SP, Kim HG, Censullo ML, Han MK. Characterization of magnesium‐dependent 3′‐processing activity for human immunodeficiency virus type 1 integrase in vitro: real‐time kinetic studies using fluorescence resonance energy transfer. Biochemistry 1995 ; 34: 10 205–14.
23. 23 Engelman A, Craigie R. Efficient magnesium dependent human immunodeficiency virus type 1 integrase activity. J Virol 1995 ; 69: 5908–11.
24. 24 Miller M, Bor YC, Bushman FD. Target DNA capture by HIV‐1 integration complexes. Curr Biol 1995 ; 5: 1047–56.
25. 25 Vink C, Lutzke RAP, Plasterk RHA. Formation of a stable complex between the human immunodeficiency virus integrase protein and viral DNA. Nucleic Acids Res 1994 ; 22: 4103–10.
26. 26 Agapkina J, Smolov M, Barbe S, Zubin E, Zatsepin T, Deprez E, et al. Probing of HIV‐1 integrase/DNA interactions using novel analogs of viral DNA. J Biol Chem 2006 ; 281: 11 530–40.
27. 27 Yi J, Asante‐Appiah E, Skalka AM. Divalent cations stimulate preferential recognition of a viral DNA end by HIV‐1 integrase. Biochemistry 1999 ; 38: 8458–68.
28. 28 Esposito D, Craigie R. Sequence specificity of viral end DNA binding by HIV‐1 integrase reveals critical regions for protein/DNA interaction. EMBO J 1998 ; 17: 5832–43.
29. 29 Diamond TL, Bushman FD. Role of metal ions in catalysis by HIV integrase analyzed using a quantitative PCR disintegration assay. Nucleic Acids Res 2006 ; 34: 6116–25.
30. 30 Grobler JA, Stillmock K, Hu B, Witmer M, Felock P, Espeseth AS, et al. Diketo acid inhibitor mechanism and HIV‐1 integrase: implications for metal binding in the active site of phosphotransferase enzymes. Proc Natl Acad Sci USA 2002 ; 99: 6661–6.