Improved method to raise polyclonal antibody using enhanced green fluorescent protein transgenic mice

Acta Biochimica et Biophysica Sinica

Volumn 40, Issue 2, 2008, Pages 111-115

  Ren, Jianke ^a,b   Wang, Long ^c   Liu, Guoxiang ^a,b   Zhang, Wen ^a   Sheng, Zhejin ^c,d   Wang, Zhugang ^c   Fei, Jian ^c,d  

^a INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c Shanghai Nan Fang Model Organism Research Center   (China)

^d TONGJI UNIVERSITY   (China)

Author keywords

EGFP; Polyclonal antibody; Transgenic mice

Indexed keywords

ANIMALIA; MUS; MUS MUSCULUS;

EID: 39749199761     PISSN: 16729145     EISSN: 17457270     Source Type: Journal    
DOI: 10.1111/j.1745-7270.2008.00381.x     Document Type: Article

References (20)

1. 1 Lerner RA, Green N, Alexander H, Liu FT, Sutcliffe JG, Shinnick TM. Chemically synthesized peptides predicted from the nucleotide sequence of the hepatitis B virus genome elicit antibodies reactive with the native envelope protein of Dane particles. Proc Natl Acad Sci USA 1981, 78: 3403–3407.
2. 2 Kirkley JE, Goldstein AL, Naylor PH. Effect of peptide‐carrier coupling on peptide‐specific immune responses. Immunobiology 2001, 203: 601–615.
3. 3 Edwards RJ, Singleton AM, Murray B P, Davies DS, Boobis AR. Short synthetic peptides exploited for reliable and specific targeting of antibodies to the C‐termini of cytochrome P450 enzymes. Biochem Pharmacol 1995, 49: 39–47.
4. 4 Beekman NJ, Schaaper WM, Turkstra JA, Meloen RH. Highly immunogenic and fully synthetic peptide‐carrier constructs targetting GnRH. Vaccine 1999, 17: 2043–2050.
5. 5 Riveros‐Moreno V, Beddell C, Moncada S. Nitric oxide synthase. Structural studies using anti‐peptide antibodies. Eur J Biochem 1993, 215: 801–808.
6. 6 Bernard D, Nicolas C, Maurizis JC, Betail G. A new method of preparing hapten‐carrier immunogens by coupling with Saccharomyces cerevisiae by periodate oxidation. J Immunol Methods 1983, 61: 351–357.
7. 7 Hansen PR, Flyge H, Holm A, Lauritzen E, Larsen BD. Photochemical conjugation of peptides to carrier proteins using 1, 2,3‐thiadiazole‐4‐carboxylic acid. Immunoreactivity of free C‐terminal epitope with specific antibodies. Int J Pept Protein Res 1996, 47: 419–426.
8. 8 Castrop J, Verbeek S, Hofhuis F, Clevers H. Circumvention of tolerance for the nuclear T cell protein TCF‐1 by immunization of TCF‐1 knock‐out mice. Immunobiology 1995, 193: 281–287.
9. 9 Simabuco FM, Carromeu C, Farinha‐Arcieri LE, Tamura RE, Ventura AM. Production of polyclonal antibodies against the human respiratory syncytial virus nucleoprotein and phosphoprotein expressed in Escherichia coli. Protein Expr Purif 2007, 53: 209–215.
10. 10 Ray MK, Wang G, Barrish J, Finegold MJ, DeMayo FJ. Immuno‐histochemical localization of mouse Clara cell 10–KD protein using antibodies raised against the recombinant protein. J Histochem Cytochem 1996, 44: 919–927.
11. 11 Marikar FM, Sun QM, Hua ZC. Production of the polyclonal anti‐human metallothionein 2A antibody with recombinant protein technology. Acta Biochim Biophys Sin 2006, 38: 305–309.
12. 12 Yu H, Yang Y, Zhang W, Xie YH, Qin J, Wang Y, Zheng HB et al. Expression and purification of recombinant SARS coronavirus spike protein. Acta Biochim Biophys Sin 2003, 35: 774–778.
13. 13 Song BL, Qi W, Wang CH, Yang JB, Yang XY, Lin ZX, Li BL. Preparation of an anti‐Cdx‐2 antibody for analysis of different species Cdx‐2 binding to acat2 promoter. Acta Biochim Biophys Sin 2003, 35: 6–12.
14. 14 Hawkins NC, Ellis GC, Bowerman B, Garriga G. MOM‐5 frizzled regulates the distribution of DSH‐2 to control C. elegans asymmetric neuroblast divisions. Dev Biol 2005, 284: 246–259.
15. 15 Gearing AJ, Bird CR, Callus M, Thorpe R. The effect of primary immunization and concanavalin A on the production of monoclonal natural antibodies. Hybridoma 1986, 5: 243–247.
16. 16 Peeters JM, Hazendonk TG, Beuvery EC, Tesser GI. Comparison of four bifunctional reagents for coupling peptides to proteins and the effect of the three moieties on the immunogenicity of the conjugates. J Immunol Methods 1989, 120: 133–143.
17. 17 Tam JP. Synthetic peptide vaccine design: Synthesis and properties of a high‐density multiple antigenic peptide system. Proc Natl Acad Sci USA 1988, 85: 5409–5413.
18. 18 Gomara MJ, Riedemann S, Vega I, Ibarra H, Ercilla G, Haro I. Use of linear and multiple antigenic peptides in the immunodiagnosis of acute hepatitis A virus infection. J Immunol Methods 2000, 234: 23–34.
19. 19 Tam JP, Clavijo P, Lu YA, Nussenzweig V, Nussenzweig R, Zavala F. Incorporation of T and B epitopes of the circumsporozoite protein in a chemically defined synthetic vaccine against malaria. J Exp Med 1990, 171: 299–306.
20. 20 Liacopoulos P, Halpern BN, Neveu T. Immune tolerance: Its mechanisms and its specificity. Rev Fr Etud Clin Biol 1964, 22: 118–125.