Non-specific adsorption of fish immunoglobulin M (IgM) to blocking reagents on ELISA plate wells

Diseases of Aquatic Organisms

Volumn 78, Issue 1, 2007, Pages 55-59

  Kim, W S ^a   Nishizawa, T ^a   Yoshimizu, M ^a  

^a HOKKAIDO UNIVERSITY   (Japan)

Author keywords

Adsorption; Antibody detection; ELISA; Enzyme linked immunosorbent assay; Fish serum; IgM; Immunoglobulin M

Indexed keywords

ADSORPTION; ANTIBODY; CYPRINID; ENZYME; FLATFISH; IMMUNOASSAY; SALMONID;

ANIMALIA; BOVINAE; COPROSMA KAUENSIS; CYPRINIDAE; CYPRINUS CARPIO; GLYCINE MAX; ONCORHYNCHUS MASOU; ONCORHYNCHUS MYKISS; PARALICHTHYS OLIVACEUS; PLEURONECTOIDEI;

IMMUNOGLOBULIN M;

ADSORPTION; ANIMAL; ANIMAL DISEASE; ARTICLE; BLOOD; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; FISH; IMMUNOLOGY; METHODOLOGY; STANDARD;

ADSORPTION; ANIMALS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FISHES; IMMUNOGLOBULIN M;

EID: 39749182836     PISSN: 01775103     EISSN: 16161580     Source Type: Journal    
DOI: 10.3354/dao01843     Document Type: Article

References (15)

1. Dixon PF, Hattenberger-Baudouy AM, Way K (1994) Detection of carp antibodies to spring viraemia of carp virus by a competitive immunoassay. Dis Aquat Org 19:181-186
2. Guo FC, Woo PTK (2004) Detection and quantification of Spironucleus barkhanus in experimentally infected Atlantic salmon Salmo salar. Dis Aquat Org 61:175-178
3. Höglund J, Pilström L (1995) Mechanical isolation and characterization of antigens from adult Anguillicola crassus. Fish Shellfish Immun 5:51-60
4. Kibenge MT, Opazo B, Rojas AH, Kibenge FSB (2002) Serological evidence of infectious salmon anaemia virus (ISAV) infection in farmed fishes, using an indirect enzyme-linked immunosorbent assay (ELISA). Dis Aquat Org 51:1-11
5. Knopf K, Naser K, van der Heijden MHT, Taraschewski H (2000) Evaluation of an ELISA and immunoblotting for studying the humoral immune response in Anguillicola crassus infected European eel Anguilla anguilla. Dis Aquat Org 43:39-48
6. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
7. LaPatra SE (1996) The use of serological techniques for virus surveillance and certification of finfish. Annu Rev Fish Dis 6:15-28
8. Nishida H, Yoshimizu M, Ezura Y (1998a) Detection of antibody against lymphocystis disease virus in Japanese flounder by enzyme linked immunosorbent assay. Fish Pathol 33:207-211
9. Nishida H, Enokida T, Hiramatsu N, Hara A, Yoshimizu M (1998b) Purification of immunoglobulin M (IgM) in serum of Japanese flounder (Paralichthys olivaceus). Bull Fac Fish Hokkaido Univ 49:157-164
10. Okuda R, Nishizawa T, Yoshimizu M (2006) Infection-cycles of Renibacterium salmoninarum in hatcheries of masu salmon Oncorhynchus masou by monitoring specific antibodies. Fish Pathol 41:175-178
11. Olesen NJ, Lorenzen N, Jørgensen PEV (1991) Detection of rainbow trout antibody to Egtved virus by enzyme-linked immunosorbent assay (ELISA), immunofluorescence (IF), and plaque neutralization tests (50% PNT). Dis Aquat Org 10:31-38
12. Swain P, Nayak SK (2003) Comparative sensitivity of different serological tests for seromonitoring and surveillance of Edwardsiella tarda infection of Indian major carps. Fish Shellfish Immun 15:333-340
13. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from Polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350-4354
14. Watanabe K, Suzuki S, Nishizawa T, Suzuki K, Yoshimizu M, Ezura Y (1998) Control strategy for viral nervous necrosis of barfin flounder. Fish Pathol 33:445-446
15. Yoshimizu M, Direkbusarakom S, Nomura T, Ezura Y, Kimura T (1992) Detection of antibody against Aeromonas salmonicida in the serum of salmonid fish by the enzyme linked immunosorbent assay. Fish Pathol 27:73-82