Standard Culture of Human Embryonic Stem Cells

Human Embryonic Stem Cells: The Practical Handbook

Volumn , Issue , 2007, Pages 53-79

  Crook, Jeremy M ^a   Horne, Rachel ^a   Colman, Alan ^a  

^a ES Cell International   (Singapore)

Author keywords

Cryopreservation; Cryostatsis; Culture; Disaggregation; Feeder; Feeder free; Freezing; Human embryonic stem cell; Microdissection; Subculture; Thawing; Vitrification

Indexed keywords

EID: 39749181701     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9780470511619.ch5     Document Type: Chapter

References (16)

1. Amit M, V Margulets, H Segev, K Shariki, I Laevsky, R Coleman and J Itskovitz-Eldor. (2003). Human feeder layers for human embryonic stem cells. Biol Reprod 68: 2150-2156.
2. Bongso A, CY Fong, SC Ng and S Ratnam. (1994). Isolation and culture of inner cell mass cells from human blastocysts. Human Reprod 9: 2110-2117.
3. Choo A, J Padmanabhan, A Chin, WJ Fong and SKW Oh. (2006) Immortalized feeders for the scale-up of human embryonic stem cells in feeder and feeder-free conditions. J Biotech 122: 130-141.
4. Crook JM and L Kravets (2005) Cell preservation method. Patent International Publication Number WO2005/118785 A1.
5. Ellerstrom C, R Strehl, K Moya, K Andersson, C Bergh, K Lundin, J Hyllner and H Semb (2006) Derivation of xeno-free human ES cell line. Stem Cells Express. June Epub ahead of print.
6. Koivisto H, M Hyvarinen, AM Stromberg, J Inzunza, E Matilainen, M Mikkola, O Hovatta and H Teerijoki. (2004) Cultures of human embryonic stem cells: serum replacement medium or serum-containing medium and the effect of basic fibroblast growth factor. Reprod Biomed Online 9: 330-337.
7. Ludwig TE, ME Levenstein, JM Jones, WT Berggren, ER Mitchen, JL Frane, LJ Crandall, CA Daigh, KR Conard, MS Piekarczyk, RA Llanas and JA Thompson. (2006) Derivation of human embryonic stem cells in defined conditions. Nat Biotechnol 24: 185-187.
8. Noaksson K, N Zoric, X Zeng, MS Rao, J Hyllner, H Semb, M Kubista and P Sartipy. (2005) Monitoring differentiation of human embryonic stem cells using real-time PCR. Stem Cells 23: 1460-1467.
9. Rall WF (2003) Avoidance of microbial cross-contamination of cryopreserved gametes, embryos, cells and tissues during storage in liquid nitrogen. The Embryologists' 6: 4-15.
10. Reubinoff BE, MF Pera, G Vajta and A Trounson. (2001) Effective cryopreservation of human embryonic stem cells by the open pulled straw vitrification method. Human Reprod 16: 2187-2194.
11. Reubinoff BE, MF Pera, CY Fong, A Trounson and A Bongso. (2000) Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Nat Biotechnol 18: 399-404.
12. Richards M, Tan S, Fong CY, Biswas A, Chan WK, Bongso A. (2003) Comparative evaluation of various human feeders for prolonged undifferentiated growth of human embryonic stem cells. Stem Cells 21: 546-556.
13. Richards M, CY Fong, WK Chan, PC Wong, A Bongso. (2002) Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells. Nat Biotechnol 20: 882-883.
14. Thomson JA, J Itskovitz-Eldor, SS Shapiro, MA Waknitz, JJ Swiergiel, VS Marshall and JM Jones. (1998) Embryonic stem cell lines derived from human blastocysts. Science 282: 1145-1147.
15. Vajta G, P Holm, M Kuwayama, PJ Booth, H Jacobsen, T Greve, and H Callesen. (1998) Open pulled straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos. Mol Reprod Dev 51: 53-58.
16. Xu C, MS Inokuma, J Denham, K Golds, P Kundu, JD Gold and MK Carpenter. (2001) Feederfree growth of undifferentiated human embryonic stem cells. Nat Biotechnol 19: 971-974.