DelsGate, a robust and rapid gene deletion construction method

Fungal Genetics and Biology

Volumn 45, Issue 4, 2008, Pages 379-388

  García Pedrajas, María D ^a   Nadal, Marina ^a   Kapa, Laura B ^a   Perlin, Michael H ^b   Andrews, David L ^a   Gold, Scott E ^a  

^a UNIVERSITY OF GEORGIA   (United States)

^b UNIVERSITY OF LOUISVILLE   (United States)

Author keywords

DelsGate; Fungal transformation; Gateway; Gene deletion; Ustilago maydis

Indexed keywords

ENDONUCLEASE;

ARTICLE; ASCOMYCETES; BASIDIOMYCETES; CLONING VECTOR; CONTROLLED STUDY; CORN; DELETION MUTANT; DNA FLANKING REGION; ESCHERICHIA COLI; FUNGAL GENE; FUNGUS MUTANT; GENE CONSTRUCT; GENE DELETION; GENE TECHNOLOGY; GENETIC PROCEDURES; GENETIC TRANSFORMATION; HOMOLOGOUS RECOMBINATION; IN VITRO STUDY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT DISEASE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REPRODUCIBILITY; USTILAGO MAYDIS;

ASCOMYCOTA; CLONING, MOLECULAR; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA, FUNGAL; ESCHERICHIA COLI; GENE DELETION; GENES, FUNGAL; GENETIC VECTORS; GENETICS, MICROBIAL; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; REPRODUCIBILITY OF RESULTS; USTILAGO;

ASCOMYCOTA; ESCHERICHIA COLI; USTILAGO MAYDIS;

EID: 39749164592     PISSN: 10871845     EISSN: 10960937     Source Type: Journal    
DOI: 10.1016/j.fgb.2007.11.001     Document Type: Article

References (13)

1. Carroll A.M., Sweigard J.A., and Valent B. Improved vectors for selecting resistance to hygromycin. Fungal Genet. Newsl. 41 (1994) 22
2. Catlett N.L., Lee B., Yoder O.C., and Turgeon B.G. Split-marker recombination for efficient targeted deletion of fungal genomes. Fungal Genet. Newsl. 50 (2002) 9-11
3. Church G.M., and Gilbert W. Genomic sequencing. Proc. Natl. Acad. Sci. USA 81 (1984) 1991-1995
4. Davidson R.C., Blankenship J.R., Kraus P.R., de Jesus Berrios M., Hull C.M., D'Souza C., Wang P., and Heitman J. A PCR-based strategy to generate integrative targeting alleles with large regions of homology. Microbiology 148 (2002) 2607-2615
5. García-Pedrajas M.D., and Gold S.E. Fungal dimorphism regulated gene expression in Ustilago maydis: II* filament down-regulated genes. Mol. Plant Pathol. 5 (2004) 295-307
6. Gold S.E., Brogdon S.M., Mayorga M.E., and Kronstad J.W. The Ustilago maydis regulatory subunit of a cAMP-dependent protein kinase is required for gall formation in maize. Plant Cell 9 (1997) 1585-1594
7. Hewald S., Linne U., Scherer M., Marahiel M.A., Kämper J., and Bölker M. Identification of a gene cluster for biosynthesis of mannosylerythritol lipids in the basidiomycetous fungus Ustilago maydis. Appl. Environ. Microbiol. 72 (2006) 5469-5477
8. Kämper J. A PCR-based system for highly efficient generation of gene replacement mutants in Ustilago maydis. Mol. Genet. Genomics 271 (2004) 103-110
9. Kämper J., Kahmann R., Bölker M., Ma L.J., et al. Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis. Nature 444 (2006) 97-101
10. Martínez-Espinoza A.D., García-Pedrajas M.D., and Gold S.E. The Ustilaginales as plant pests and model systems. Fungal Genet. Biol. 35 (2002) 1-20
11. Mayorga M.E., and Gold S.E. The ubc2 gene of Ustilago maydis encodes a putative novel adaptor protein required for filamentous growth, pheromone response and virulence. Mol. Microbiol. 41 (2001) 1365-1379
12. Suzuki Y., Kagawa N., Fujino T., Sumiya T., Andoh T., Ishikawa K., Kimura R., Kemmochi K., Ohta T., and Tanaka S. A novel high-throughput (HTP) cloning strategy for site-directed designed chimeragenesis and mutation using the Gateway cloning system. Nucleic Acids Res. 33 (2005) e109
13. Walhout A.J., Temple G.F., Brasch M.A., Hartley J.L., Lorson M.A., van den Heuvel S., and Vidal M. GATEWAY recombination cloning: application to the cloning of large numbers of open reading frames or ORFeomes. Methods Enzymol. 328 (2000) 575-592