RT in situ PCR detection of MART-1 and TRP-2 mRNA in formalin-fixed, paraffin-embedded tissues of melanoma and nevi

Modern Pathology

Volumn 21, Issue 3, 2008, Pages 326-333

  Itakura, Eijun ^a   Huang, Rong Rong ^a   Wen, Duan Ren ^a   Paul, Eberhard ^b   Wünsch, Peter H ^b   Cochran, Alistair J ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b KLINIKUM NÜRNBERG   (Germany)

Author keywords

MART 1; Melanocytic neoplasms; Melanoma; RT in situ PCR; Tyrosinase related protein 2

Indexed keywords

DNA POLYMERASE; MELAN A; TYROSINASE RELATED PROTEIN 2;

ARTICLE; CONTROLLED STUDY; GENE SEQUENCE; HUMAN; IMMUNOHISTOCHEMISTRY; MELANOMA; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; THERMUS THERMOPHILUS;

ANTIGENS, NEOPLASM; CELL LINE, TUMOR; FORMALDEHYDE; HUMANS; IMMUNOHISTOCHEMISTRY; INTRAMOLECULAR OXIDOREDUCTASES; MELANOMA; NEOPLASM PROTEINS; NEVUS; PARAFFIN EMBEDDING; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA, NEOPLASM; SENSITIVITY AND SPECIFICITY; SKIN NEOPLASMS; TISSUE FIXATION;

EID: 39749156951     PISSN: 08933952     EISSN: 15300285     Source Type: Journal    
DOI: 10.1038/modpathol.3801008     Document Type: Article

References (42)

1. Li PX, Cheng L, Wen DR, et al. Demonstration of cytoplasmic tyrosinase mRNA in tissue-cultured cells by reverse transcription (RT) in situ polymerase chain reaction (PCR) and RT PCR in situ hybridization. Diagn Mol Pathol 1997;6:26-33.
2. Guo J, Wen DR, Huang RR, et al. Detection of multiple melanoma-associated markers in melanoma cell lines by RT in situ PCR. Exp Mol Pathol 2003;74:140-147.
3. Guo J, Cheng L, Wen DR, et al. Detection of tyrosinase mRNA in formalin-fixed, paraffin-embedded archival sections of melanoma, using the reverse transcriptase in situ polymerase chain reaction. Diagn Mol Pathol 1998;7:10-15.
4. Qian X, Lloyd RV. Recent developments in signal amplification methods for in situ hybridization. Diagn Mol Pathol 2003;12:1-13.
5. Coulie PG, Brichard V, Van Pel A, et al. A new gene coding for a differentiation antigen recognized by autologous cytolytic T lymphocytes on HLA-A2 melanomas. J Exp Med 1994;180:35-42.
6. Kawakami Y, Eliyahu S, Delgado CH, et al. Cloning of the gene coding for a shared human melanoma antigen recognized by autologous T cells infiltrating into tumor. Proc Natl Acad Sci USA 1994;91:3515-3519.
7. Kawakami Y, Eliyahu S, Delgado CH, et al. Identification of a human melanoma antigen recognized by tumor-infiltrating lymphocytes associated with in vivo tumor rejection. Proc Natl Acad Sci USA 1994;91:6458-6462.
8. Tsukamoto K, Jackson IJ, Urabe K, et al. A second tyrosinase-related protein, TRP-2, is a melanogenic enzyme termed DOPAchrome tautomerase. EMBO J 1992;11:519-526.
9. Hearing VJ, Tsukamoto K, Urabe K, et al. Functional properties of cloned melanogenic proteins. Pigment Cell Res 1992;5:264-270.
10. Golub SH, Hanson DC, Sulit HL, et al. Comparison of histocompatibility antigens on cultured human tumor cells and fibroblasts by quantitative antibody absorption and sensitivity to cell-mediated cytotoxicity. J Natl Cancer Inst 1976;56:167-170.
11. Chee DO, Boddie AW, Roth JA, et al. Production of melanoma-associated antigen(s) by a defined malignant melanoma cell strain grown in chemically defined medium. Cancer Res 1976;36:1503-1509.
12. Irie RF, Irie K, Morton DL. A membrane antigen common to human cancer and fetal brain tissues. Cancer Res 1976;36:3510-3517.
13. Nuovo GJ. Co-labeling using in situ PCR: a review. J Histochem Cytochem 2001;49:1329-1339.
14. Adam PJ, Boyd R, Tyson KL, et al. Comprehensive proteomic analysis of breast cancer cell membranes reveals unique proteins with potential roles in clinical cancer. J Biol Chem 2003;278:6482-6489.
15. Sarantou T, Chi DD, Garrison DA, et al. Melanoma-associated antigens as messenger RNA detection markers for melanoma. Cancer Res 1997;57:1371-1376.
16. Busam KJ, Chen YT, Old LJ, et al. Expression of melan-A (MART1) in benign melanocytic nevi and primary cutaneous malignant melanoma. Am J Surg Pathol 1998;22:976-982.
17. Busam KJ, Iversen K, Coplan KA, et al. Immunoreactivity for A103, an antibody to melan-A (Mart-1), in adrenocortical and other steroid tumors. Am J Surg Pathol 1998;22:57-63.
18. Fetsch PA, Marincola FM, Abati A. The new melanoma markers: MART-1 and Melan-A (the NIH experience). Am J Surg Pathol 1999;23:607-610.
19. Pisarra P, Lupetti R, Palumbo A, et al. Human melanocytes and melanomas express novel mRNA isoforms of the tyrosinase-related protein-2/DOPA-chrome tautomerase gene: molecular and functional characterization. J Invest Dermatol 2000;115:48-56.
20. Virador V, Matsunaga N, Matsunaga J, et al. Production of melanocyte-specific antibodies to human melanosomal proteins: expression patterns in normal human skin and in cutaneous pigmented lesions. Pigment Cell Res 2001;14:289-297.
21. Becker B, Roesch A, Hafner C, et al. Discrimination of melanocytic tumors by cDNA array hybridization of tissues prepared by laser pressure catapulting. J Invest Dermatol 2004;122:361-368.
22. Nishioka E, Funasaka Y, Kondoh H, et al. Expression of tyrosinase, TRP-1 and TRP-2 in ultraviolet-irradiated human melanomas and melanocytes: TRP-2 protects melanoma cells from ultraviolet B induced apoptosis. Melanoma Res 1999;9:433-443.
23. Rad HH, Yamashita T, Jin HY, et al. Tyrosinase-related proteins suppress tyrosinase-mediated cell death of melanocytes and melanoma cells. Exp Cell Res 2004;298:317-328.
24. Kageshita T, Kawakami Y, Hirai S, et al. Differential expression of MART-1 in primary and metastatic melanoma lesions. J Immunother 1997;20:460-465.
25. Cormier JN, Hijazi YM, Abati A, et al. Heterogeneous expression of melanoma-associated antigens and HLA-A2 in metastatic melanoma in vivo. Int J Cancer 1998;75:517-524.
26. Jungbluth AA, Busam KJ, Gerald WL, et al. A103: an anti-melan-a monoclonal antibody for the detection of malignant melanoma in paraffin-embedded tissues. Am J Surg Pathol 1998;22:595-602.
27. Durda PJ, Dunn IS, Rose LB, et al. Induction of "antigen silencing" in melanomas by oncostatin M: down-modulation of melanocyte antigen expression. Mol Cancer Res 2003;1:411-419.
28. Kurnick JT, Ramirez-Montagut T, Boyle LA, et al. A novel autocrine pathway of tumor escape from immune recognition: melanoma cell lines produce a soluble protein that diminishes expression of the gene encoding the melanocyte lineage melan-A/MART-1 antigen through down-modulation of its promoter. J Immunol 2001;167:1204-1211.
29. Du J, Miller AJ, Widlund HR, et al. MLANA/MART1 and SILV/PMEL17/GP100 are transcriptionally regulated by MITF in melanocytes and melanoma. Am J Pathol 2003;163:333-343.
30. Kono M, Dunn IS, Durda PJ, et al. Role of the mitogen-activated protein kinase signaling pathway in the regulation of human melanocytic antigen expression. Mol Cancer Res 2006;4:779-792.
31. Pawelec G. Tumour escape: antitumour effectors too much of a good thing? Cancer Immunol Immunother 2004;53:262-274.
32. Stanta G, Schneider C. RNA extracted from paraffin-embedded human tissues is amenable to analysis by PCR amplification. BioTechniques 1991;11:304, 306, 308.
33. Stanta G, Bonin S. RNA quantitative analysis from fixed and paraffin-embedded tissues: membrane hybridization and capillary electrophoresis. BioTechniques 1998;24:271-276.
34. Godfrey TE, Kim SH, Chavira M, et al. Quantitative mRNA expression analysis from formalin-fixed, paraffin-embedded tissues using 5′ nuclease quantitative reverse transcription-polymerase chain reaction. J Mol Diagn 2000;2:84-91.
35. Abrahamsen HN, Steiniche T, Nexo E, et al. Towards quantitative mRNA analysis in paraffin-embedded tissues using real-time reverse transcriptase-polymerase chain reaction: a methodological study on lymph nodes from melanoma patients. J Mol Diagn 2003;5:34-41.
36. Hully JR. In situ PCR. In: Innis MA, Gelfand DH, Sninsky JJ (eds). PCR Applications: Protocols for Functional Genomics. Academic Press: London, 1999, pp 169-194.
37. Morel G, Raccurt M. PCR/RT-PCR In Situ: Light and Electron Microscopy. CRC Press: Boca Raton, FL, 2003.
38. Peters J, Krams M, Wacker HH, et al. Detection of rare RNA sequences by single-enzyme in situ reverse transcription-polymerase chain reaction. High-resolution analyses of interleukin-6 mRNA in paraffin sections of lymph nodes. Am J Pathol 1997;150:469-476.
39. Bostick PJ, Morton DL, Turner RR, et al. Prognostic significance of occult metastases detected by sentinel lymphadenectomy and reverse transcriptase-polymerase chain reaction in early-stage melanoma patients. J Clin Oncol 1999;17:3238-3244.
40. Rimoldi D, Lemoine R, Kurt AM, et al. Detection of micrometastases in sentinel lymph nodes from melanoma patients: direct comparison of multimarker molecular and immunopathological methods. Melanoma Res 2003;13:511-520.
41. Takeuchi H, Morton DL, Kuo C, et al. Prognostic significance of molecular upstaging of paraffin-embedded sentinel lymph nodes in melanoma patients. J Clin Oncol 2004;22:2671-2680.
42. Abrahamsen HN, Sorensen BS, Nexo E, et al. Pathologic assessment of melanoma sentinel nodes: a role for molecular analysis using quantitative real-time reverse transcription-PCR for MART-1 and tyrosinase messenger RNA. Clin Cancer Res 2005;11:1425-1433.