The role of the Serratia marcescens SdeAB multidrug efflux pump and TolC homologue in fluoroquinolone resistance studied via gene-knockout mutagenesis

Microbiology

Volumn 154, Issue 2, 2008, Pages 454-461

  Begic, Sanela ^a   Worobec, Elizabeth A ^a  

^a UNIVERSITY OF MANITOBA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CHLORAMPHENICOL; CIPROFLOXACIN; ETHIDIUM BROMIDE; MEMBRANE PROTEIN; MULTIDRUG RESISTANCE PROTEIN; NOVOBIOCIN; PROTEIN HASF; PROTEIN SDEAB; PROTEIN SDER; QUINOLINE DERIVED ANTIINFECTIVE AGENT; REGULATOR PROTEIN; TOLC PROTEIN; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM ISOLATION; DRUG SENSITIVITY; FLUORESCENCE SPECTROSCOPY; KNOCKOUT GENE; MINIMUM INHIBITORY CONCENTRATION; MULTIDRUG RESISTANCE; NONHUMAN; PRIORITY JOURNAL; SERRATIA MARCESCENS; STRAIN DIFFERENCE; WILD TYPE;

BACTERIAL OUTER MEMBRANE PROTEINS; DRUG RESISTANCE, MULTIPLE, BACTERIAL; FLUOROQUINOLONES; GENES, TRANSGENIC, SUICIDE; GENETIC COMPLEMENTATION TEST; MEMBRANE TRANSPORT PROTEINS; MICROBIAL SENSITIVITY TESTS; MUTAGENESIS; SERRATIA MARCESCENS;

ESCHERICHIA COLI; NEGIBACTERIA; SERRATIA MARCESCENS;

EID: 39749097282     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/mic.0.2007/012427-0     Document Type: Article

References (25)

1. Alekshun, M. N. & Levy, S. B. (1997). Regulation of chromosomally mediated multiple antibiotic resistance: the mar regulon. Antimicrob Agents Chemother 41, 2067-2075.
2. Aucken, H. M. & Pitt, T. L (1998). Antibiotic resistance and putative virulence factors of Serratia marcescens with respect to O and K serotypes. J Med Microbiol 47, 1105-1113.
3. Barbosa, T. M. & Levy, S. B. (2000). Differential expression of over 60 chromosomal genes in Escherichia coli by constitutive expression of MarA. J Bacteriol 182, 3467-3474.
4. Buckley, A. M., Webber, M. A., Cooles, S., Randall, L. P., La Ragione, R. M., Woodward, M. J. & Piddock, L. J. V. (2006). The AcrAB-TolC efflux system of Salmonella enterica serovar Typhimurium plays a role in pathogenesis. Cell Microbiol 8, 847-856.
5. Chen, J., Kuroda, T., Huda, M. N., Mizushima, T. & Tsuchiya, T. (2003). An RND-type multidrug efflux pump SdeXY from Serratia marcescens. J Antimicrob Chemother 52, 176-179.
6. Chollet R., Chevalier, J., Bollet, C., Pages, J. M. & Davin-Regli, A. (2004). RamA is an alternate activator of the multidrug resistance cascade in Enterobacter aerogenes. Antimicrob Agents Chemother 48, 2518-2523.
7. CLSI (2006). Performance Standards for Antimicrobial Susceptibility Testing. Supplement M100-S16. Wayne, PA: Clinical and Laboratory Standards Institute.
8. Finan, T. M., Kunkel, B., De Vos, G. F. & Signer, E., R. (1986). Second symbiotic megaplasmid in Rhizobium meliloti carrying exopolysaccharide and thiamine synthesis genes. J Bacteriol 167, 66-72.
9. Fujimaki, K., Fujii, T., Aoyama, H., Sato, K-I., Inoue, Y., Inoue, M. & Mitsuhashi, S. (1989). Quinolone resistance in clinical isolates of Serratia marcescens. Antimicrob Agents Chemother 33, 785-787.
10. Hachler, H., Cohen, S. P. & Levy, S. B. (1991). marA, a regulated locus which controls expression of chromosomal multiple antibiotic resistance in Escherichia coli. J Bacteriol 173, 5532-5538.
11. Keniga, K., Delor, I. & Cornelis, G. R. (1991). A wide-host-range suicide vector for improving reverse genetics in Gram-negative bacteria: inactivation of the blaA gene of Yersinia enterocolitica. Gene 109, 137-141.
12. Kumar, A. (2004). Characterization of RND efflux pumps of Serratia marcescens. PhD thesis, Department of Microbiology, University of Manitoba.
13. Kumar, A. & Worobec, E. A. (2005a). Cloning, sequencing and characterization of the SdeAB multidrug efflux pump of Serratia marcescens. Antimicrob Agents Chemother 49, 1495-1501.
14. Kumar, A. & Worobec, E. A. (2005b). HasF, a TolC-homolog of Serratia marcescens, is involved in energy-dependent efflux: Can J Microbiol 51, 497-500.
15. Lambert, H. P. & O'Grady, F. W. (1992). Antibiotics and Chemotherapy. Edinburgh: Churchill Livingstone.
16. Li, X-Z. & Nikaido, H. (2004). Efflux-mediated drug resistance in bacteria. Drugs 64, 159-204.
17. Ma, D., Cook, D. N., Alberti, M., Pon, N. G., Nikaido, H. & Hearst, J. E. (1995). Genes acrA and acrB encode a stress-induced efflux system of Escherichia coli. Mol Microbiol 16, 45-55.
18. Maxwell, A. & Lawson, D. M. (2003). The ATP-binding site of type II topoisomerases as a target for antibacterial drugs. Curr Top Med Chem 3, 283-303.
19. Mortimer, P. G. & Piddock, L. J. (1991). A comparison of methods used for measuring the accumulation of quinolones by Enterobacteriaceae, Pseudomonas aeruginosa and Staphylococcus aureus. J Antimicrob Chemother 28, 639-653.
20. Nagakubo, S., Nishino, K., Hirata, T. & Yamaguchi, A. (2002). The putative response regulator BaeR stimulates multidrug resistance of Escherichia coli via a novel multidrug exporter system, MdtABC. J Bacteriol 184, 4161-4167.
21. Pearson, J. P., Pesci, E. C. & Iglewski, B. H. (1997). Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes. J Bacteriol 179, 5756-5757.
22. Posadas, D. M., Martin, F. A., Sabio y Garcia, J. V., Spera, J. M., Delpino, M. V., Baldi, P., Campos, E, Cravero, S. L. & Zorreguieta, A. (2007). The TolC homologue of Brucella suis is involved in resistance to antimicrobial compounds and virulence. Infect Immun 75, 379-389.
23. Pumbwe, L. & Piddock, J. V. (2002). Identification and molecular characterization of CmeB, a Campylobacter jejuni multidrug efflux pump. FEMS Microbiol Lett 206, 185-189.
24. Ruzin, A., Keeney, D. & Bradford, P. A. (2007). AdeABC multidrug efflux pump is associated with decreased susceptibility to tigecycline in Acinetobacter calcoaceticus-Acinetobacter baumannii complex. J Antimicrob Chemother 59, 1001-1004.
25. van Amsterdam, K., Bart, A. & van der Ende A. (2005). A Helicobacter pylori TolC efflux pump confers resistance to metronidazole. Antimicrob Agents Chemother 49, 1477-1482.