HIV-1 DNA load analysis in peripheral blood lymphocytes and monocytes from naïve and HAART-treated individuals

Journal of Infection

Volumn 56, Issue 3, 2008, Pages 219-225

  Gibellini, Davide ^a   Borderi, Marco ^a   De Crignis, Elisa ^a   Cicola, Ronny ^a   Cimatti, Laura ^a   Vitone, Francesca ^a   Chiodo, Francesco ^a   Re, Maria Carla ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

Highly active antiretroviral therapy; HIV 1; Monocytes; Peripheral blood lymphocytes; Reservoir

Indexed keywords

ANTIRETROVIRUS AGENT; RNA DIRECTED DNA POLYMERASE INHIBITOR; VIRUS DNA; VIRUS RNA;

ADULT; AGED; ARTICLE; CD4 LYMPHOCYTE COUNT; CONTROLLED STUDY; CORRELATION ANALYSIS; DNA DETERMINATION; DRUG EFFICACY; DRUG RESPONSE; HIGHLY ACTIVE ANTIRETROVIRAL THERAPY; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTION; HUMAN IMMUNODEFICIENCY VIRUS INFECTED PATIENT; LONG TERM CARE; LYMPHOCYTE; MAJOR CLINICAL STUDY; MONOCYTE; NONHUMAN; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SERODIAGNOSIS; VIRUS LOAD;

ADULT; AGED; ANTIRETROVIRAL THERAPY, HIGHLY ACTIVE; CD4 LYMPHOCYTE COUNT; CROSS-SECTIONAL STUDIES; DNA, VIRAL; FEMALE; HIV INFECTIONS; HIV-1; HUMANS; LYMPHOCYTES; MALE; MIDDLE AGED; MONOCYTES; RNA, VIRAL; VIRAL LOAD;

EID: 39549113870     PISSN: 01634453     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jinf.2008.01.001     Document Type: Article

References (45)

1. Haggerty C., Pitt E., and Siliciano R. The latent reservoir for HIV in resting cells and other viral reservoirs during chronic infection: insight from treatment and treatment-interruption trials. Curr Opin HIV AIDS 1 (2006) 62-68
2. Finzi D., Hermankova M., Pierson T., Carruth L.M., Buck C., Chaisson R.E., et al. Identification of a reservoir for HIV-1 in patients on highly active antiretroviral therapy. Science 278 (1997) 1295-1300
3. Perelson A.S., Essunger P., Cao Y., Vesanen M., Hurley A., Saksela K., et al. Decay characteristics of HIV-1-infected compartments during combination therapy. Nature 387 (1997) 188-191
4. Kulkosky J., and Bray S. HAART-persistent HIV-1 latent reservoirs: their origin, mechanisms of stability and potential strategies for eradication. Curr HIV Res 4 (2006) 199-208
5. Noe A., Plum J., and Verhofstede C. The latent HIV-1 reservoir in patients undergoing HAART: an archive of pre-HAART drug resistance. J Antimicrob Chemother 55 (2005) 410-412
6. Han Y., Lassen K., Monie D., Sedaghat A.R., Shimoji S., Liu X., et al. Resting CD4+ T cells from human immunodeficiency virus type 1 (HIV-1)-infected individuals carry integrated HIV-1 genomes within actively transcribed host genes. J Virol 78 (2004) 6122-6133
7. Lewinski M.K., Bisgrove D., Shinn P., Chen H., Hoffmann C., Hannenhalli S., et al. Genome-wide analysis of chromosomal features repressing human immunodeficiency virus transcription. J Virol 79 (2005) 6610-6619
8. Larsen K., Han Y., Zhou Y., Siliciano J., and Siliciano R.F. The multifactorial nature of HIV-1 latency. Trends Mol Med 10 (2004) 525-531
9. Nickle D.C., Jensen M.A., Shriner D., Brodie S.J., Frenkel L.M., Mittler J.E., et al. Evolutionary indicators of human immunodeficiency virus type 1 reservoirs and compartments. J Virol 77 (2003) 5540-5546
10. Bailey J.R., Blankson J.N., Wind-Rotolo M., and Siliciano R.F. Mechanisms of HIV-1 escape from immune responses and antiretroviral drugs. Curr Opin Immunol 16 (2004) 470-476
11. Crowe S., Zhu T., and Muller W.A. The contribution of monocyte infection and trafficking to viral persistence, and maintenance of the viral reservoir in HIV infection. J Leuk Biol 74 (2003) 635-641
12. Gartner S., Markovits P., Markovitz D.M., Kaplan M.H., Gallo R.C., and Popovic M. The role of mononuclear phagocytes in HTLV-III/LAV infection. Science 233 (1986) 215-219
13. Zhu T. HIV-1 in peripheral blood monocytes: an underrated viral source. J Antimicr Chemiot 50 (2002) 309-311
14. Garbuglia A.R., Calcaterra S., D'Offizi G., Topino S., Narciso P., Lillo F., et al. HIV-1 DNA burden dynamics in CD4 T cells and monocytes in patients undergoing a transient therapy interruption. J Med Virol 74 (2004) 373-381
15. Fulcher J.A., Hwangbo Y., Zioni R., Nickle D., Lin X., Heath L., et al. Compartmentalization of human immunodeficiency virus type 1 between blood monocytes and CD4+ T cells during infection. J Virol 78 (2004) 7883-7893
16. Brostrom C., Visco-Comandini U., Yun Z., Sonnerborg A., Brostrom C., and Visco-Comandini U. Longitudinal quantification of human immunodeficiency virus type 1 DNA and RNA in long-term nonprogressors. J Infect Dis 179 (1999) 1542-1548
17. Shiramizu B., Gartner S., Williams A., Shikuma C., Ratto-Kim S., Watters M., et al. Circulating proviral HIV DNA and HIV-associated dementia. AIDS 19 (2005) 45-52
18. Bailey J.R., Sedaghat A.R., Kieffer T., Brennan T., Lee P.K., Wind-Rotolo M., et al. Residual human immunodeficiency virus type 1 viremia in some patients on antiretroviral therapy is dominated by a small number of invariant clones rarely found in circulating CD4+ T cells. J Virol 80 (2006) 6441-6457
19. Perfetto S.P., and McCrary G. Immunophenotyping and assessment of cell function by three-color flow cytometry of peripheral blood lymphocytes. In: Michael N., and Kim J.H. (Eds). HIV protocols (1999), Humana Press, Totowa NJ 397-407
20. Gibellini D., Vitone F., Schiavone P., Ponti C., La Placa M., and Re M.C. Quantitative detection of human immunodeficiency virus type 1 (HIV-1) proviral DNA in peripheral blood mononuclear cells by SYBR green real-time PCR technique. J Clin Virol 29 (2004) 282-289
21. Gibellini D., Gardini F., Vitone F., Schiavone P., Furlini G., and Re M.C. Simultaneous detection of HCV and HIV-1 by SYBR Green real time multiplex RT-PCR technique in plasma samples. Mol Cell Probes 20 (2006) 223-229
22. Gibellini D., Vitone F., Gori E., La Placa M., and Re M.C. Quantitative detection of human immunodeficiency virus type 1 (HIV-1) viral load by SYBR green real time RT-PCR technique in HIV-1 seropositive patients. J Virol Methods 115 (2004) 183-189
23. Bohling S.D., and Elelitoba-Johnson K.S.J. Development of PCR-based assays for the detection of chromosomal translocations using SYBR Green I. In: Meuer S., Wittwer C., and Nakagawara K. (Eds). Rapid cycle real time PCR: methods and applications (2001), Springer, Berlin 231-239
24. Re M.C., Vitone F., Bon I., Schiavone P., and Gibellini D. Meaning of DNA detection during the follow-up of HIV-1 infected patients: a brief review. New Microbiol 29 (2006) 81-88
25. Ibáñez A., Puig T., Elias J., Clotet B., Ruiz L., and Martínez M.A. Quantification of integrated and total HIV-1 DNA after long-term highly active antiretroviral therapy in HIV-1-infected patients. AIDS 13 (1999) 1045-1049
26. Ngo-Giang-Huong N., Deveau C., Da Silva I., Pellegrin I., Venet A., Harzic M., et al. Proviral HIV-1 DNA in subjects followed since primary HIV-1 infection who suppress plasma viral load after one year of highly active antiretroviral therapy. AIDS 15 (2001) 665-673
27. De Rossi A., Walker A.S., De Forni D., and Gibb D.M. Paediatric European Network for Treatment of AIDS (PENTA). Biphasic decay of cell-associated HIV-1 DNA in HIV-1-infected children on antiretroviral therapy. AIDS 16 (2002) 1961-1963
28. Hatzakis A.E., Touloumi G., Pantazis N., Anastassopoulou C.G., Katsarou O., Karafoulidou A., et al. Cellular HIV-1 DNA load predicts HIV-RNA rebound and the outcome of highly active antiretroviral therapy. AIDS 18 (2004) 2261-2267
29. Sarmati L., Parisi S.G., Nicastri E., D'Ettorre G., Palmisano L., Andreotti M., et al. Association between cellular human immunodeficiency virus DNA level and immunological parameters in patients with undetectable plasma viremia level during highly active antiretroviral therapy. J Clin Microbiol 43 (2005) 6183-6185
30. Finzi D., Blankson J., Siliciano J.D., Margolick J.B., Chadwick K., Pierson T., et al. Latent infection of CD4+ T cells provides a mechanism for lifelong persistence of HIV-1, even in patients on effective combination therapy. Nat Med 5 (1999) 512-517
31. Strain M.C., Gunthard H.F., Havlir D.V., Ignacio C.C., Smith D.M., Leigh-Brown A.J., et al. Heterogeneous clearance rates of long-lived lymphocytes infected with HIV: intrinsic stability predicts lifelong persistence. Proc Natl Acad Sci USA 100 (2003) 4819-4824
32. Chun T.W., Justement J.S., Pandya P., Hallahan C.W., McLaughlin M., Liu S., et al. Relationship between the size of HIV-1 reservoir in peripheral blood CD4+ T cells and CD4+:CD8+ T-cell ratios in aviremic HIV-1 infected individuals receiving long-term highly active antiretroviral therapy. J Infect Dis 185 (2002) 1672-1676
33. McDermott J.L., Giri A.A., Martini I., Bono M., Giacomini M., Campelli A., et al. Level of human immunodeficiency virus DNA in peripheral blood mononuclear cells correlates with efficacy of antiretroviral therapy. J Clin Microbiol 37 (1999) 2361-2365
34. Lafeuillade A., Poggi C., Chadapaud S., Hittinger G., Khiri H., and Halfon P. Impact of immune interventions on proviral HIV-1 DNA decay in patients receiving highly active antiretroviral therapy. HIV Med 2 (2001) 189-194
35. Riva E., Antonelli G., Scagnolari C., Pistello M., Capobianchi M.R., Monforte A., et al. HIV DNA load and level of immunosuppression in treatment-naïve HIV-1 infected patients. J Infect Dis 187 (2003) 1826-1828
36. Lillo F.B., Grasso M.A., Lodini S., Bellotti M.G., and Colucci G. Few modifications of the Cobas Amplicor HIV Monitor 1.5 test allow reliable quantitation of HIV-1 proviral load in peripheral blood mononuclear cells. J Virol Methods 120 (2004) 201-205
37. Tierney C., Lathey J.L., Christopherson C., Bettendorf D.M., D'Aquila R.T., Hammer S.M., et al. Prognostic value of baseline human immunodeficiency virus type 1 DNA measurement for disease progression in patients receiving nucleoside therapy. J Infect Dis 187 (2003) 144-148
38. Kabamba-Mukadi B., Henrivaux P., Ruelle J., Delferrière N., Bodéus M., and Goubau P. Human immunodeficiency virus type 1 (HIV-1) proviral DNA load in purified CD4+ cells by LightCycler real-time PCR. BMC Infect Dis 5 (2005) 15-23
39. Lambotte O., Taoufik Y., De Goer M.G., Wallon C., Goujard C., and Delfraissy J.F. Detection of infectious HIV in circulating monocytes from patients on prolonged highly active antiretroviral therapy. J Acquir Immune Defic Syndr 23 (2000) 114-119
40. Sonza S., Mutimer H.P., Oelrichs R., Jardine D., Harvey K., Dunne A., et al. Monocytes harbour replication-competent, non-latent HIV-1 in patients on highly active antiretroviral therapy. AIDS 15 (2001) 17-22
41. Zhu T., Muthui D., Holte S., Nickle D., Feng F., Brodie S., et al. Evidence for human immunodeficiency virus type replication in vivo in CD14(+) monocytes and its potential role as a source of virus in patients on highly active antiretroviral therapy. J Virol 76 (2002) 707-716
42. Shen Y., Rudnik J., Cassol S., Drouin J., Cameron W., Izaguirre C.A., et al. Blood monocytes from HIV-1-infected patients do not carry proviral DNA. Clin Diagn Lab Immunol 1 (1994) 531-537
43. Aquaro S., Bagnarelli P., Guenci T., De Luca A., Clementi M., Balestra E., et al. Long-term survival and virus production in human primary macrophages infected by human immunodeficiency virus. J Med Virol 68 (2002) 479-488
44. Rivero A., Lozano J.M., Gonzalez R., García-Jurado G., Camacho A., Torres-Cisneros J., et al. Nucleoside reverse transcriptase inhibitors are able and protease inhibitors unable to induce the tolerogenic molecule HLA-G1 on monocytes from HIV-1 infected patients. Hum Immunol 68 (2007) 303-306
45. Delobel P., Sandres-Sauné K., Cazabat M., L'Faqihi F.E., Aquilina C., Obadia M., et al. Persistence of distinct HIV-1 populations in blood monocytes and naïve and memory CD4 T cells during prolonged suppressive HAART. AIDS 19 (2005) 1739-1750