Characterization of the 4D5Flu single-chain antibody with a stimulus-responsive elastin-like peptide linker: A potential reporter of peptide linker conformation

Protein Science

Volumn 17, Issue 3, 2008, Pages 527-536

  Blenner, Mark A ^a   Banta, Scott ^a  

^a Och Spine at New York Presbyterian Hospitals   (United States)

Author keywords

Diabody; Elastin like peptides; Modular assembly; Peptide conformational change; sELP; Single chain antibody; Stimulus responsive peptides

Indexed keywords

4D5FLU SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; AMINO ACID; ELASTIN LIKE PEPTIDE LINKER; FLUORESCEIN; PEPTIDE DERIVATIVE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; BINDING AFFINITY; BIOTECHNOLOGY; COMPARATIVE STUDY; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DOMAIN; ROOM TEMPERATURE; THERMODYNAMICS;

BIOSENSING TECHNIQUES; ELASTIN; FLUORESCEIN; FLUORESCENT DYES; IMMUNOGLOBULIN VARIABLE REGION; MUTATION; OSMOLAR CONCENTRATION; PEPTIDES; PROTEIN STRUCTURE, SECONDARY; TEMPERATURE;

EID: 39549094014     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1110/ps.073257308     Document Type: Article

References (38)

1. Alfthan, K., Takkinen, K., Sizmann, D., Soderlund, H., and Teeri, T.T. 1995. Properties of a single-chain antibody containing different linker peptides. Protein Eng. 8: 725-731.
2. Arndt, K.M., Muller, K.M., and Pluckthun, A. 1998. Factors influencing the dimer to monomer transition of an antibody single-chain Fv fragment. Biochemistry 37: 12918-12926.
3. Banta, S., Megeed, Z., Casali, M., Rege, K., and Yarmush, M.L. 2007. Engineering protein and peptide building blocks for nanotechnology. J. Nanosci. Nanotechnol. 7: 387-401.
4. Beerli, R.R. and Barbas, C.F. 2002. Engineering polydactyl zinc-finger transcription factors. Nat. Biotechnol. 20: 135-141.
5. Bhattacharyya, R.P., Remenyi, A., Yeh, B.J., and Lim, W.A. 2006. Domains, motifs, and scaffolds: The role of modular interactions in the evolution and wiring of cell signaling circuits. Annu. Rev. Biochem. 75: 655-680.
6. Bird, R.E., Hardman, K.D., Jacobson, J.W., Johnson, S., Kaufman, B.M., Lee, S.M., Lee, T., Pope, S.H., Riordan, G.S., and Whitlow, M. 1988. Single-chain antigen-binding proteins. Science 242: 423-426.
7. Buskirk, A.R. and Liu, D.R. 2005. Creating small-molecule-dependent switches to modulate biological functions. Chem. Biol. 12: 151-161.
8. Chin, J.W. 2006. Modular approaches to expanding the functions of living matter. Nat. Chem. Biol. 2: 304-311.
9. Cho, W.H. and Stahelin, R.V. 2005. Membrane-protein interactions in cell signaling and membrane trafficking. Annu. Rev. Biophys. Biomol. Struct. 34: 119-151.
10. Chockalingam, K., Blenner, M., and Banta, S. 2007. Design and application of stimulus-responsive peptide systems. Protein Eng. Des. Sel. 20: 155-161.
11. Chothia, C. 1974. Hydrophobic bonding and accessible surface-area in proteins. Nature 248: 338-339.
12. Dattelbaum, J.D., Looger, L.L., Benson, D.E., Sali, K.M., Thompson, R.B., and Hellinga, H.W. 2005. Analysis of allosteric signal transduction mechanisms in an engineered fluorescent maltose biosensor. Protein Sci. 14: 284-291.
13. Dueber, J.E., Yeh, B.J., Chak, K., and Lim, W.A. 2003. Reprogramming control of an allosteric signaling switch through modular recombination. Science 301: 1904-1908.
14. Essig, N.Z., Wood, J.F., Howard, A.J., Raag, R., and Whitlow, M. 1993. Crystallization of single-chain Fv proteins. J. Mol. Biol. 234: 897-901.
15. Gill, S.C. and von Hippel, P.H. 1989. Calculation of protein extinction coefficients from amino-acid sequence data. Anal. Biochem. 182: 319-326.
16. Griffiths, A.D., Malmqvist, M., Marks, J.D., Bye, J.M., Embleton, M.J., Mccafferty, J., Baier, M., Holliger, K.P., Gorick, B.D., Hughesjones, N.C., et al. 1993. Human anti-self antibodies with high specificity from phage display libraries. EMBO J. 12: 725-734.
17. Guntas, G., Mansell, T.J., Kim, J.R., and Ostermeier, M. 2005. Directed evolution of protein switches and their application to the creation of ligand-binding proteins. Proc. Natl. Acad. Sci. 102: 11224-11229.
18. Jung, S. and Pluckthun, A. 1997. Improving in vivo folding and stability of a single-chain Fv antibody fragment by loop grafting. Protein Eng. 10: 959-966.
19. Kumita, J.R., Flint, D.G., Woolley, G.A., and Smart, O.S. 2003. Achieving photo-control of protein conformation and activity: Producing a photocontrolled leucine zipper. Faraday Discuss. 122: 89-103.
20. Lim, S. and Franklin, S.J. 2006. Engineered lanthanide-binding metallohomeodomains: Designing folded chimeras by modular turn substitution. Protein Sci. 15: 2159-2165.
21. Loewenthal, R., Sancho, J., Reinikainen, T., and Fersht, A.R. 1993. Long-range surface charge-charge interactions in proteins: Comparison of experimental results with calculations from a theoretical method. J. Mol. Biol. 232: 574-583.
22. MacKenzie, C.R., Hirama, T., Deng, S.J., Bundle, D.R., Narang, S.A., and Young, N.M. 1996. Analysis by surface plasmon resonance of the influence of valence on the ligand binding affinity and kinetics of an anticarbohydrate antibody. J. Biol. Chem. 271: 1527-1533.
23. Megeed, Z., Winters, R.M., and Yarmush, M.L. 2006. Modulation of single-chain antibody affinity with temperature-responsive elastin-like polypeptide linkers. Biomacromolecules 7: 999-1004.
24. Muller, J.D., Nienhaus, G.U., Tetin, S.Y., and Voss, E.W. 1994. Ligand-binding to anti-fluorescyl antibodies - Stability of the antigen-binding site. Biochemistry 33: 6221-6227.
25. Ostermeier, M. 2005. Engineering allosteric protein switches by domain insertion. Protein Eng. Des. Sel. 18: 359-364.
26. Pawson, T. and Linding, R. 2005. Synthetic modular systems - Reverse engineering of signal transduction. FEBS Lett. 579: 1808-1814.
27. Radley, T.L., Markowska, A.I., Bettinger, B.T., Ha, J.H., and Loh, S.N. 2003. Allosteric switching by mutually exclusive folding of protein domains. J. Mol. Biol. 332: 529-536.
28. Reiersen, H. and Rees, A.R. 1999. An engineered minidomain containing an elastin turn exhibits a reversible temperature-induced IgG binding. Biochemistry 38: 14897-14905.
29. Reiersen, H. and Rees, A.R. 2000. Sodium sulphate reactivates a protein A minidomain with a short elastin β-turn. Biochem. Biophys. Res. Commun. 276: 899-904.
30. Reiersen, H., Clarke, A.R., and Rees, A.R. 1998. Short elastin-like peptides exhibit the same temperature-induced structural transitions as elastin polymers: Implications for protein engineering. J. Mol. Biol. 283: 255-264.
31. Ringler, P. and Schulz, G.E. 2003. Self-assembly of proteins into designed networks. Science 302: 106-109.
32. Sagawa, T., Azuma, T., and Sasaki, Y.C. 2007. Dynamical regulations of protein-ligand bindings at single molecular level. Biochem. Biophys. Res. Commun. 355: 770-775.
33. Skretas, G. and Wood, D.W. 2005. Regulation of protein activity with small-molecule-controlled inteins. Protein Sci. 14: 523-532.
34. Turner, D.J., Ritter, M.A., and George, A.J. 1997. Importance of the linker in expression of single-chain Fv antibody fragments: Optimisation of peptide sequence using phage display technology. J. Immunol. Methods 205: 43-54.
35. Urry, D.W. 1993. Molecular machines - How motion and other functions of living organisms can result from reversible chemical-changes. Angew. Chem. Int. Ed. Engl. 32: 819-841.
36. Urry, D.W. 1999. Elastic molecular machines in metabolism and soft-tissue restoration. Trends Biotechnol. 17: 249-257.
37. Urry, D.W., Trapane, T.L., and Prasad, K.U. 1985. Phase-structure transitions of the elastin polypentapeptide-water system within the framework of composition-temperature studies. Biopolymers 24: 2345-2356.
38. Wheeldon, I.R., Barton, S.C., and Banta, S. 2007. Bioactive proteinaceous hydrogels from designed bifunctional building blocks. Biomacromolecules 8: 2990-2994.