Correlation between API 20 STREP and multiplex PCR for identification of Enterococcus spp. isolated from Brazilian foods

Brazilian Journal of Microbiology

Volumn 38, Issue 4, 2007, Pages 617-619

  Gomes, Bruna C ^a   Esteves, Carolina T ^a   Palazzo, Izabel C V ^a   Darini, Ana Lúcia C ^a   Franco, Bernadette D G M ^a   De Martinis, Elaine C P ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

API 20 STREP; Enterococcus spp.; Foods; Identification; PCR multiplex

Indexed keywords

ENTEROCOCCUS; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM;

EID: 39449136519     PISSN: 15178382     EISSN: 16784405     Source Type: Journal    
DOI: 10.1590/S1517-83822007000400007     Document Type: Article

References (14)

1. Chingwaru, W.; Mpuchane, S.F.; Gashe, B.A. (2003). Enterococcus faecalis and Enterococcus faecium isolates from milk, beef, and chicken and their antibiotic resistance. J. Food Prot., 66, 931-936.
2. Devriese, L.A.; Pot, B.; Collins, M.D. (1993). Phenotypic identification of the genus Enterococcus and differentiation of phylogenetically distinct enterococcal species and species groups. J. Appl. Bacteriol., 75, 399-408.
3. Devriese, L.A.; Pot, B.; Damme, L.V.; Kersters, K.; Haesebrouck, F. (1995). Identification of Enterococcus species isolated from foods of animal origin. Int. J. Food Microbiol., 26, 187-197.
4. Domig, K.J.; Mayer, H.K.; Kneifel, W. (2003). Methods used for the isolation, enumeration, characterization and identification of Enterococcus spp. 2. Pheno-and genotypic criteria. Int. J. Food Microbiol., 88, 165-188.
5. Dutka-Malen, S.; Evers, S.; Courvalin, P. (1995). Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J. Clin. Microbiol., 33, 24-27.
6. Eaton, T.J.; Gasson, M.J. (2001). Molecular screening of Enterococcus virulence determinants and potential for genetic exchange between food and medical isolates. Appl. Environ. Microbiol., 67, 1628-1635.
7. Facklam, R.; Teixeira, L.M. Enterococcus. In: Murray, P.R.; Baron, E.J.; Jorgensen, J.H.; Pfaller, M.A.; Yolken, R.H. (2003). Manual Clin. Microbiol. 8 ed. Washington D.C.: ASM Press, p.422-433.
8. Foulquié-Moreno, M.R.; Sarantinopoulos, P.; Tsakalidou, E.; De Vuyst, L. (2006). The role and application of enterococci in food and health. Int. J. Food Microbiol., 106, 1-24.
9. Giraffa, G. (2002). Enterococci from foods. FEMS Microbiol. Rev., 26, 163-171.
10. Morrison, D.; Woodford, N.; Cookson, B. (1997). Enterococci as emerging pathogens of humans. J. Appl. Microbiol., 83, 89S-99S.
11. Robredo, B.; Singh, K.V.; Baquero, F.; Murray, B.E.; Torres, C. (2000). Vancomycin-resistant enterococci isolated from animals and food. Int. J. Microbiol., 54, 197-204.
12. Velasco, D.; Perez, S.; Peña, F.; Dominguez, M.A.; Cartelle, M.; Molina, F.; Moure, R.; Villanueva, R.; Bou, G. (2004). Lack of correlation between phenotypic techniques and PCR-based genotypic methods for identification of Enterococcus spp. Bacteriol., 49, 151-156.
13. Willey, B.M.; Jones, R.N.; McGeer, A.; Witte, W.; French, G.; Roberts, R.B.; Jenkins, S.G.; Nadler, H.; Low, D.E. (1999). Practical approach to the identification of clinically relevant Enterococcus species. Diagn. Microbiol. Infect. Dis., 34, 165-171.
14. Winston, L.G.; Pang, S.; Haller, B.L.; Wong, M.; Chambers III, H.F.; Perdreau-Remington, F. (2004). API 20 STREP identification system may incorrectly speciate enterococci with low level resistance to vancomycin. Diagn. Microbiol. Infect. Dis., 48, 287-288.